Effects of immobilization by entrapment in alginate and scale-up on paclitaxel and baccatin III production in cell suspension cultures ofTaxus baccata

2005 ◽  
Vol 89 (6) ◽  
pp. 647-655 ◽  
Author(s):  
Salima Bentebibel ◽  
Elisabet Moyano ◽  
Javier Palazón ◽  
Rosa M. Cusidó ◽  
Mercedes Bonfill ◽  
...  
1996 ◽  
Vol 14 (9) ◽  
pp. 1129-1132 ◽  
Author(s):  
Yukihito Yukimune ◽  
Homare Tabata ◽  
Yosuke Higashi ◽  
Yasuhiro Hara

2018 ◽  
pp. 01-07 ◽  
Author(s):  
Samrin Shaikh ◽  
Varsha Shriram ◽  
Tushar Khare and Vinay Kumar

Helicteres isora L. (Malvaceae) is a medicinal plant highly used in traditional therapeutic practices. It has shown wide-spectrum therapeutic activities including anti-plasmodial, hypoglycemic, hypolipidemic, hepatoprotective, antinociceptive, antioxidant and anti-HIV. Present investigation was undertaken with an objective of establishment of cell suspension cultures of this plant which can be further used for in vitro production of desired secondary metabolites and their further scale-up. Seed dormancy was broken using sulfuric acid and seedlings were raised in vitro. MS medium supplemented with 2,4-D (0.5 mg/L) produced maximum callus from the nodal explants. The callus produced was used as an explant for the establishment of suspension cultures. MS medium without any supplement was proved best for the establishment of cell suspension cultures of H. isora. To the best of our knowledge, this is the first report on H. isora cell suspension culture establishment.    


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