Scale up production of isoflavonoids in cell suspension cultures ofPueraria tuberosagrown in shake flasks and bioreactor

2009 ◽  
Vol 9 (3) ◽  
pp. 267-271 ◽  
Author(s):  
Varsha Sharma ◽  
Shaily Goyal ◽  
Kishan Gopal Ramawat
Keyword(s):  
Cell Suspension ◽  
Scale Up ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Shake Flasks

Effects of immobilization by entrapment in alginate and scale-up on paclitaxel and baccatin III production in cell suspension cultures ofTaxus baccata

2005 ◽  
Vol 89 (6) ◽  
pp. 647-655 ◽  
Author(s):  
Salima Bentebibel ◽  
Elisabet Moyano ◽  
Javier Palazón ◽  
Rosa M. Cusidó ◽  
Mercedes Bonfill ◽  
...  
Keyword(s):  
Cell Suspension ◽  
Scale Up ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Baccatin Iii

A fermenter for plant cell suspension cultures

1970 ◽  
Vol 16 (4) ◽  
pp. 223-226 ◽  
Author(s):  
I. A. Veliky ◽  
S. M. Martin
Keyword(s):  
Cell Suspension ◽  
Plant Cell ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Plant Cell Suspension Cultures ◽  
Plant Cell Suspension ◽  
Metabolic Products ◽  
Shake Flasks

A conical glass V-fermenter for plant cell suspension cultures is described. This V-fermenter has proved useful for the production of biomass and metabolic products. It also is ideally suited for the preparation and maintenance of actively growing cultures to be used as inocula for shake flasks and fermenters. An outline of the procedure for isolating and "conditioning" plant cell suspension cultures is presented.

scholarly journals Establishment of Callus and Cell Suspension Cultures of Helicteres isora L.

2018 ◽  
pp. 01-07 ◽  
Author(s):  
Samrin Shaikh ◽  
Varsha Shriram ◽  
Tushar Khare and Vinay Kumar
Keyword(s):  
Cell Suspension ◽  
Wide Spectrum ◽  
Scale Up ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Ms Medium ◽  
In Vitro Production ◽  
Helicteres Isora ◽  
Therapeutic Activities

Helicteres isora L. (Malvaceae) is a medicinal plant highly used in traditional therapeutic practices. It has shown wide-spectrum therapeutic activities including anti-plasmodial, hypoglycemic, hypolipidemic, hepatoprotective, antinociceptive, antioxidant and anti-HIV. Present investigation was undertaken with an objective of establishment of cell suspension cultures of this plant which can be further used for in vitro production of desired secondary metabolites and their further scale-up. Seed dormancy was broken using sulfuric acid and seedlings were raised in vitro. MS medium supplemented with 2,4-D (0.5 mg/L) produced maximum callus from the nodal explants. The callus produced was used as an explant for the establishment of suspension cultures. MS medium without any supplement was proved best for the establishment of cell suspension cultures of H. isora. To the best of our knowledge, this is the first report on H. isora cell suspension culture establishment.    

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