scholarly journals Removal of poly-histidine fusion tags from recombinant proteins purified by expanded bed adsorption

2005 ◽  
Vol 92 (4) ◽  
pp. 501-513 ◽  
Author(s):  
N. Abdullah ◽  
H.A. Chase
Keyword(s):  
Recombinant Proteins ◽  
Expanded Bed Adsorption ◽  
Expanded Bed

Ligand Choice in Purifications of Recombinant Proteins by Expanded Bed Adsorption

2001 ◽  
Vol 73 (6) ◽  
pp. 721-722 ◽  
Author(s):  
Robert H. Clemmitt ◽  
Norhafizah Abdullah ◽  
Howard A. Chase
Keyword(s):  
Recombinant Proteins ◽  
Expanded Bed Adsorption ◽  
Expanded Bed

Expanded bed adsorption as a fast technique for the large-scale purification of the complete isoform pool of Ber e 1, the major allergen from Brazil nuts

2006 ◽  
Vol 50 (3) ◽  
pp. 275-281 ◽  
Author(s):  
Evelien L. van Boxtel ◽  
Gerrit A. van Koningsveld ◽  
Stef J. Koppelman ◽  
Lambertus A. M. van den Broek ◽  
Alfons G. J. Voragen ◽  
...  
Keyword(s):  
Large Scale ◽  
Major Allergen ◽  
Expanded Bed Adsorption ◽  
Expanded Bed ◽  
Brazil Nuts

Interaction of mammalian cell culture broth with adsorbents in expanded bed adsorption of monoclonal antibodies

1999 ◽  
Vol 34 (2) ◽  
pp. 159-165 ◽  
Author(s):  
J. Feuser ◽  
M. Halfar ◽  
D. Lütkemeyer ◽  
N. Ameskamp ◽  
M.-R. Kula ◽  
...  
Keyword(s):  
Cell Culture ◽  
Monoclonal Antibodies ◽  
Mammalian Cell ◽  
Mammalian Cell Culture ◽  
Culture Broth ◽  
Expanded Bed Adsorption ◽  
Expanded Bed

scholarly journals A study of ion-exchange chromatography in an expanded bed for bovine albumin recovery

2009 ◽  
Vol 52 (2) ◽  
pp. 427-436 ◽  
Author(s):  
João Batista Severo Jr. ◽  
Roberto Rodrigues de Souza ◽  
José Carlos Curvelo Santana ◽  
Elias Basile Tambourgi
Keyword(s):  
Ion Exchange ◽  
Exchange Resin ◽  
Binding Capacity ◽  
Ion Exchange Resin ◽  
Exchange Chromatography ◽  
Expanded Bed Adsorption ◽  
Expanded Bed ◽  
Bed Height ◽  
Salt Addition ◽  
Bed Expansion

In the present work, the effect of bed expansion on BSA adsorption on Amberlite IRA 410 ion-exchange resin was studied. The hydrodynamic behavior of an expanded bed adsorption column on effects of the biomolecules and salt addition and temperature were studied to optimize the conditions for BSA recovery on ion-exchange resin. Residence time distribution showed that HEPT, axial dispersion and the Pecletl number increased with temperature and bed height, bed voidage and linear velocity. The binding capacity of the resin increased with bed height. The Amberlite IRA 410 ion-exchange showed an affinity for BSA with a recovery yield of 78.36 % of total protein.

Direct process integration of extraction and expanded bed adsorption in the recovery of crocetin derivatives from Fructus Gardenia

2007 ◽  
Vol 858 (1-2) ◽  
pp. 220-226 ◽  
Author(s):  
Min Zhang ◽  
Ping Hu ◽  
Qionglin Liang ◽  
Huihua Yang ◽  
Qingfei Liu ◽  
...  
Keyword(s):  
Process Integration ◽  
Expanded Bed Adsorption ◽  
Direct Process ◽  
Expanded Bed
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