Purification of soluble human epidermal growth factor (hEGF) from recombinant Escherichia coli culture broth by using expanded-bed adsorption chromatography

Biotechnology and Applied Biochemistry ◽

10.1042/ba20020113 ◽

2003 ◽

Vol 38 (1) ◽

pp. 9 ◽

Author(s):

Yoon Seok Lee ◽

Chang Woo Suh ◽

Seung Kook Park ◽

Eun Kyu Lee

Keyword(s):

Escherichia Coli ◽

Growth Factor ◽

Epidermal Growth Factor ◽

Culture Broth ◽

Adsorption Chromatography ◽

Recombinant Escherichia Coli ◽

Expanded Bed Adsorption ◽

Expanded Bed ◽

Human Epidermal Growth Factor ◽

Epidermal Growth

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Partial purification properties of human epidermal growth factor from recombinant Escherichia coli by expanded bed adsorption

World Journal of Microbiology and Biotechnology ◽

10.1007/s11274-004-1554-4 ◽

2005 ◽

Vol 21 (1) ◽

pp. 51-57 ◽

Author(s):

Wang-Yu Tong ◽

Shan-Jing Yao ◽

Zi-Qiang Zhu

Keyword(s):

Escherichia Coli ◽

Growth Factor ◽

Epidermal Growth Factor ◽

Recombinant Escherichia Coli ◽

Expanded Bed Adsorption ◽

Partial Purification ◽

Expanded Bed ◽

Human Epidermal Growth Factor ◽

Epidermal Growth

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Recombinant human epidermal growth factor inclusion body solubilization and refolding at large scale using expanded-bed adsorption chromatography from Escherichia coli

Protein Expression and Purification ◽

10.1016/j.pep.2008.02.020 ◽

2008 ◽

Vol 60 (1) ◽

pp. 7-14 ◽

Author(s):

K. Sharma ◽

P.V. Cherish Babu ◽

P. Sasidhar ◽

V.K. Srinivas ◽

V. Krishna Mohan ◽

...

Keyword(s):

Escherichia Coli ◽

Growth Factor ◽

Epidermal Growth Factor ◽

Inclusion Body ◽

Large Scale ◽

Adsorption Chromatography ◽

Expanded Bed Adsorption ◽

Expanded Bed ◽

Human Epidermal Growth Factor ◽

Epidermal Growth

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Cultivation of recombinant Escherichia coli for secretory production of human epidermal growth factor under control of PL promoter

Enzyme and Microbial Technology ◽

10.1016/j.enzmictec.2006.06.001 ◽

2007 ◽

Vol 40 (4) ◽

pp. 708-715 ◽

Author(s):

Haiyi Zhang ◽

Zhimin Li ◽

Yue Qian ◽

Qian Zhang ◽

Peng Du ◽

...

Keyword(s):

Escherichia Coli ◽

Growth Factor ◽

Epidermal Growth Factor ◽

Recombinant Escherichia Coli ◽

Human Epidermal Growth Factor ◽

Epidermal Growth ◽

Secretory Production

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Human epidermal growth factor excreted by recombinant Escherichia coli K-12 has the correct N-terminus and is fully bioactive

Process Biochemistry ◽

10.1016/s0032-9592(99)00024-2 ◽

1999 ◽

Vol 35 (1-2) ◽

pp. 1-5 ◽

Author(s):

R.C Huang ◽

E Lam ◽

Y.H Chen ◽

J Hackett ◽

T.L Lam ◽

...

Keyword(s):

Escherichia Coli ◽

Growth Factor ◽

Epidermal Growth Factor ◽

Recombinant Escherichia Coli ◽

Human Epidermal Growth Factor ◽

Epidermal Growth ◽

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Recovery of Microquantities of Human Epidermal Growth Factor fromEscherichia coliHomogenate andPichia pastorisCulture Medium using Expanded Bed Adsorption

Separation Science and Technology ◽

10.1080/01496395.2013.867351 ◽

2014 ◽

Vol 49 (5) ◽

pp. 702-708 ◽

Author(s):

Ira Amira Rosti ◽

Ramakrishnan Nagasundara Ramanan ◽

Joo Shun Tan ◽

Tau Chuan Ling ◽

Arbakariya B. Ariff

Keyword(s):

Growth Factor ◽

Epidermal Growth Factor ◽

Expanded Bed Adsorption ◽

Expanded Bed ◽

Human Epidermal Growth Factor ◽

Epidermal Growth

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Production of excreted human epidermal growth factor (hEGF) by an efficient recombinant Escherichia coli system

Process Biochemistry ◽

10.1016/s0032-9592(99)00013-8 ◽

1999 ◽

Vol 34 (9) ◽

pp. 893-900 ◽

Author(s):

S Sivakesava ◽

Z.N Xu ◽

Y.H Chen ◽

J Hackett ◽

R.C Huang ◽

...

Keyword(s):

Escherichia Coli ◽

Growth Factor ◽

Epidermal Growth Factor ◽

Recombinant Escherichia Coli ◽

Human Epidermal Growth Factor ◽

Epidermal Growth

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Pilot scale production of a recombinant human epidermal growth factor, secreted by Bacillus brevis , using expanded bed adsorption

Journal of Industrial Microbiology & Biotechnology ◽

10.1038/sj.jim.2900570 ◽

1998 ◽

Vol 21 (4-5) ◽

pp. 208-214 ◽

Author(s):

A Miyauchi ◽

S Ebisu ◽

K Uchida ◽

M Yoshida ◽

M Ozawa ◽

...

Keyword(s):

Growth Factor ◽

Epidermal Growth Factor ◽

Pilot Scale ◽

Expanded Bed Adsorption ◽

Scale Production ◽

Expanded Bed ◽

Bacillus Brevis ◽

Human Epidermal Growth Factor ◽

Pilot Scale Production ◽

Epidermal Growth

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Production of human epidermal growth factor by an ampicillin resistant recombinant Escherichia coli strain

Biotechnology Letters ◽

10.1007/bf00128619 ◽

1994 ◽

Vol 16 (9) ◽

pp. 885-890 ◽

Author(s):

V. B. Yadwad ◽

S. Wilson ◽

O. P. Ward

Keyword(s):

Escherichia Coli ◽

Growth Factor ◽

Epidermal Growth Factor ◽

Coli Strain ◽

Recombinant Escherichia Coli ◽

Human Epidermal Growth Factor ◽

Epidermal Growth

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Factors influencing excretive production of human epidermal growth factor (hEGF) with recombinant Escherichia coli K12 system

Bioprocess and Biosystems Engineering ◽

10.1007/s004490000218 ◽

2000 ◽

Vol 23 (6) ◽

pp. 669-674 ◽

Author(s):

Xu Zhinan ◽

Liu Gang ◽

Cen Peilin ◽

W. K. R. Wong

Keyword(s):

Escherichia Coli ◽

Growth Factor ◽

Epidermal Growth Factor ◽

Recombinant Escherichia Coli ◽

Human Epidermal Growth Factor ◽

Escherichia Coli K12 ◽

Factors Influencing ◽

Epidermal Growth

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Production of phoA promoter-controlled human epidermal growth factor in fed-batch cultures of Escherichia coli YK537 (pAET-8)

Process Biochemistry ◽

10.1016/j.procbio.2005.03.010 ◽

2005 ◽

Vol 40 (9) ◽

pp. 3068-3074 ◽

Author(s):

Yonggang Wang ◽

Hongzhen Ding ◽

Peng Du ◽

Renbao Gan ◽

Qin Ye

Keyword(s):

Escherichia Coli ◽

Growth Factor ◽

Epidermal Growth Factor ◽

Fed Batch ◽

Batch Cultures ◽

Human Epidermal Growth Factor ◽

Epidermal Growth

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