Tailor-made CRISPR/Cas system for highly efficient targeted gene replacement in the rice blast fungus

2015 ◽  
Vol 112 (12) ◽  
pp. 2543-2549 ◽  
Author(s):  
Takayuki Arazoe ◽  
Kennosuke Miyoshi ◽  
Tohru Yamato ◽  
Tetsuo Ogawa ◽  
Shuichi Ohsato ◽  
...  
Keyword(s):  
Rice Blast ◽  
Rice Blast Fungus ◽  
Gene Replacement ◽  
Highly Efficient ◽  
Blast Fungus ◽  
Targeted Gene Replacement

Tailor-made TALEN system for highly efficient targeted gene replacement in the rice blast fungus

2015 ◽  
Vol 112 (7) ◽  
pp. 1335-1342 ◽  
Author(s):  
Takayuki Arazoe ◽  
Tetsuo Ogawa ◽  
Kennosuke Miyoshi ◽  
Tohru Yamato ◽  
Shuichi Ohsato ◽  
...  
Keyword(s):  
Rice Blast ◽  
Rice Blast Fungus ◽  
Gene Replacement ◽  
Highly Efficient ◽  
Blast Fungus ◽  
Targeted Gene Replacement

scholarly journals A Novel Role for Catalase B in the Maintenance of Fungal Cell-Wall Integrity During Host Invasion in the Rice Blast Fungus Magnaporthe grisea

2007 ◽  
Vol 20 (5) ◽  
pp. 568-580 ◽  
Author(s):  
Pari Skamnioti ◽  
Catherine Henderson ◽  
Ziguo Zhang ◽  
Zena Robinson ◽  
Sarah Jane Gurr
Keyword(s):  
Rice Blast ◽  
Magnaporthe Grisea ◽  
Large Subunit ◽  
Rice Blast Fungus ◽  
Gene Replacement ◽  
Fungal Cell ◽  
Antioxidant Genes ◽  
Blast Fungus

Asexual spores of the rice blast fungus germinate to produce a specialized and melanized infection structure, the appressorium, which is pivotal to successful plant penetration. To investigate whether Magnaporthe grisea counteracts the toxic burst of H2O2 localized beneath the site of attempted invasion, we examined the temporal expression of five candidate antioxidant genes. Of these, the putatively secreted large subunit catalase CATB gene was 600-fold up-regulated in vivo, coincident with penetration, and moderately up-regulated in vitro, in response to exogenous H2O2. Targeted gene replacement of CATB led to compromised pathogen fitness; the catB mutant displayed paler pigmentation and accelerated hyphal growth but lower biomass, poorer sporulation, fragile conidia and appressoria, and impaired melanization. The catB mutant was severely less pathogenic than Guy 11 on barley and rice, and its infectivity was further reduced on exposure to H2O2. The wild-type phenotype was restored by the reintroduction of CATB into the catB mutant. We found no evidence to support a role for CATB in detoxification of the host-derived H2O2 at the site of penetration. Instead, we demonstrated that CATB plays a part in strengthening the fungal wall, a role of particular importance during forceful entry into the host.

scholarly journals MST12 Regulates Infectious Growth But Not Appressorium Formation in the Rice Blast Fungus Magnaporthe grisea

2002 ◽  
Vol 15 (3) ◽  
pp. 183-192 ◽  
Author(s):  
Gyungsoon Park ◽  
Chaoyang Xue ◽  
Li Zheng ◽  
Stephen Lam ◽  
Jin-Rong Xu
Keyword(s):  
Transcription Factor ◽  
Rice Blast ◽  
Magnaporthe Grisea ◽  
Rice Blast Fungus ◽  
Gene Replacement ◽  
Mitogen Activated Protein Kinase ◽  
Kinase Gene ◽  
Blast Fungus ◽  
Mitogen Activated Protein ◽  
Barley Leaves

In the rice blast fungus Magnaporthe grisea, a mitogen-activated protein kinase gene, PMK1, is known to regulate ap-pressorium formation and infectious hyphae growth. Since PMK1 is homologous to the FUS3 and KSS1 genes that regulate the transcription factor STE12 in yeast, we functionally characterized the STE12 homologue in M. grisea (MST12). A polymerase chain reaction-based approach was used to isolate the MST12 gene that is homologous to yeast STE12. Four mst12 deletion mutants were isolated by gene replacement. No obvious defect in vegetative growth, conidiation, or conidia germination was observed in mst12 mutants. However, mst12 mutants were nonpathogenic on rice and barley leaves. In contrast to pmk1 mutants that did not form appressoria, mst12 mutants produced typical dome-shaped and melanized appressoria. However, the ap-pressoria formed by mst12 mutants failed to penetrate onion epidermal cells. When inoculated through wound sites, mst12 mutants failed to cause spreading lesions and appeared to be defective in infectious growth. These data indicate that MST12 may function downstream of PMK1 to regulate genes involved in infectious hyphae growth. A transcription factor or factors other than MST12 must exist in M. grisea and function downstream from PMK1 for ap-pressorium formation.

scholarly journals Investigating the cell and developmental biology of plant infection by the rice blast fungus Magnaporthe oryzae

2021 ◽  
pp. 103562
Author(s):  
Alice Bisola Eseola ◽  
Lauren S. Ryder ◽  
Míriam Osés-Ruiz ◽  
Kim Findlay ◽  
Xia Yan ◽  
...  
Keyword(s):  
Developmental Biology ◽  
Magnaporthe Oryzae ◽  
Rice Blast ◽  
Rice Blast Fungus ◽  
Plant Infection ◽  
Blast Fungus

scholarly journals A novel lipoxygenase from rice. Primary structure and specific expression upon incompatible infection with rice blast fungus.

1994 ◽  
Vol 269 (5) ◽  
pp. 3755-3761 ◽  
Author(s):  
Y.L. Peng ◽  
Y. Shirano ◽  
H. Ohta ◽  
T. Hibino ◽  
K. Tanaka ◽  
...  
Keyword(s):  
Primary Structure ◽  
Rice Blast ◽  
Rice Blast Fungus ◽  
Specific Expression ◽  
Blast Fungus

scholarly journals Tanzawaic acids I–L: Four new polyketides from Penicillium sp. IBWF104-06

2014 ◽  
Vol 10 ◽  
pp. 251-258 ◽  
Author(s):  
Louis P Sandjo ◽  
Eckhard Thines ◽  
Till Opatz ◽  
Anja Schüffler
Keyword(s):  
Soil Sample ◽  
Magnaporthe Oryzae ◽  
Rice Blast ◽  
Rice Blast Fungus ◽  
Fungal Strain ◽  
Conidial Germination ◽  
Penicillium Sp ◽  
Culture Filtrates ◽  
Blast Fungus ◽  
New Compounds

Four new polyketides have been identified in culture filtrates of the fungal strain Penicillium sp. IBWF104-06 isolated from a soil sample. They are structurally based on the same trans-decalinpentanoic acid skeleton as tanzawaic acids A–H. One of the new compounds was found to inhibit the conidial germination in the rice blast fungus Magnaporthe oryzae at concentrations of 25 μg/mL.

scholarly journals Differential loss of effector genes in three recently expanded pandemic clonal lineages of the rice blast fungus

BMC Biology ◽  
2020 ◽  
Vol 18 (1) ◽  
Author(s):  
Sergio M. Latorre ◽  
C. Sarai Reyes-Avila ◽  
Angus Malmgren ◽  
Joe Win ◽  
Sophien Kamoun ◽  
...  
Keyword(s):  
Rice Blast ◽  
Rice Blast Fungus ◽  
Effector Genes ◽  
Blast Fungus

scholarly journals A Homeobox Gene Is Essential for Conidiogenesis of the Rice Blast Fungus Magnaporthe oryzae

2010 ◽  
Vol 23 (4) ◽  
pp. 366-375 ◽  
Author(s):  
Wende Liu ◽  
Shiyong Xie ◽  
Xinhua Zhao ◽  
Xin Chen ◽  
Wenhui Zheng ◽  
...  
Keyword(s):  
Magnaporthe Oryzae ◽  
Rice Blast ◽  
Homeobox Gene ◽  
Gene Replacement ◽  
Fungal Genome ◽  
Protein Signaling ◽  
Homeodomain Proteins ◽  
Conidial Production ◽  
Targeted Gene Replacement ◽  
Severity Of The Disease

Magnaporthe oryzae starts its infection by the attachment of pyriform conidia on rice tissues, and severity of the disease epidemic is proportional to the quantity of conidia produced in the rice blast lesions. However, the mechanism of conidial production is not well understood. Homeodomain proteins play critical roles in regulating various growth and developmental processes in fungi and other eukaryotes. Through targeted gene replacement, we find that deletion of HTF1, one of seven homeobox genes in the fungal genome, does not affect mycelial growth but causes total defect of conidial production. Further observation revealed that the Δhtf1 mutant produces significantly more conidiophores, which curve slightly near the tip but could not develop sterigmata-like structures. Although the Δhtf1 mutant fails to form conidia, it could still develop melanized appressoria from hyphal tips and infect plants. The expression level of HTF1 is significantly reduced in the Δmgb1 G-β and ΔcpkA deletion mutant, and the ACR1 but not CON7 gene that encodes transcription factor required for normal conidiogenesis is significantly downregulated in the Δhtf1 mutant. These data suggest that the HTF1 gene is essential for conidiogenesis, and may be functionally related to the trimeric G-protein signaling and other transcriptional regulators that are known to be important for conidiation in M. oryzae.

scholarly journals Two Novel Transcriptional Regulators Are Essential for Infection-related Morphogenesis and Pathogenicity of the Rice Blast Fungus Magnaporthe oryzae

2011 ◽  
Vol 7 (12) ◽  
pp. e1002385 ◽  
Author(s):  
Xia Yan ◽  
Ya Li ◽  
Xiaofeng Yue ◽  
Congcong Wang ◽  
Yawei Que ◽  
...  
Keyword(s):  
Magnaporthe Oryzae ◽  
Rice Blast ◽  
Transcriptional Regulators ◽  
Rice Blast Fungus ◽  
Blast Fungus
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