Characteristics of carbohydrate degradation and the rate-limiting step in anaerobic digestion

1985 ◽  
Vol 27 (10) ◽  
pp. 1482-1489 ◽  
Author(s):  
Tatsuya Noike ◽  
Ginro Endo ◽  
Juu-En Chang ◽  
Jun-Ichi Yaguchi ◽  
Jun-Ichiro Matsumoto
2011 ◽  
Vol 64 (2) ◽  
pp. 375-383 ◽  
Author(s):  
S. I. Pérez-Elvira ◽  
M. Fdz-Polanco ◽  
F. Fdz-Polanco

Anaerobic digestion (AD) is the preferred option to stabilize sludge. However, the rate limiting step of solids hydrolysis makes it worth modifing the conventional mesophilic AD in order to increase the performance of the digester. The main strategies are to introduce a hydrolysis pre-treatment, or to modify the digestion temperature. Among the different pre-treatment alternatives, the thermal hydrolysis (TH) at 170 °C for 30 min, and the ultrasounds pre-treatment (US) at 30 kJ/kg TS were selected for the research, while for the non-conventional anaerobic digestion, the thermophilic (TAD) and the two-stage temperature phased AD (TPAD) were considered. Four pilot plants were operated, with the same configuration and size of anaerobic digester (200 L, continuously fed). The biogas results show a general increase compared to the conventional digestion, being the highest production per unit of digester for the process combining the thermal pre-treatment and AD (1.4 Lbiogas/Ldigester·day compared to the value of 0.26 obtained in conventional digesters). The dewaterability of the digestate became enhanced for processes TH + AD and TPAD when compared with the conventional digestate, while it became worse for processes US + AD and TAD. In all the research lines, the viscosity in the digester was smaller compared to the conventional (which is a key factor for process performance and economics), and both thermal pre-treatment and thermophilic digestion (TAD and TPAD) assure a pathogen free digestate.


1989 ◽  
Vol 21 (4-5) ◽  
pp. 175-186 ◽  
Author(s):  
C. Y. Lin ◽  
T. Noike ◽  
H. Furumai ◽  
J. Matsumoto

Results obtained from experiments on two-phase anaerobic digestion using a high concentration of a volatile fatty acid (VFA) mixture were used to elucidate the kinetic mechanism of the methanogenesis process. The mixture consisted of the major intermediate products of anaerobic digestion, i.e., acetic acid (HAc), propionic acid (HPr) and butyric acid (HBu). The relationship between the rate of substrate utilization and substrate concentration in the digesters was in the form of a Michaelis-Menten equation. The rate-limiting step of the methanogenesis process, i.e., the conversion of HAc to methane, was speeded up in the digesters and this was proved kinetically. A method for determining kinetic constants for substrate-specific microorganisms was suggested. A simulation model for predicting the effluent substrate concentration was demonstrated. The effluent substrate concentration of an anaerobic digester fed by a multisubstrate was found to be simulatively predictable from its influent component substrates.


RSC Advances ◽  
2019 ◽  
Vol 9 (51) ◽  
pp. 29570-29578
Author(s):  
Xiaofang Pan ◽  
Lina Wang ◽  
Nan Lv ◽  
Jing Ning ◽  
Mingdian Zhou ◽  
...  

Physical structures of sludge are critical factors determining the performance of the anaerobic digestion process, especially for the rate-limiting step, methanogenesis.


2013 ◽  
Vol 134 ◽  
pp. 391-395 ◽  
Author(s):  
Jingwei Ma ◽  
Craig Frear ◽  
Zhi-wu Wang ◽  
Liang Yu ◽  
Quanbao Zhao ◽  
...  

1978 ◽  
Vol 39 (02) ◽  
pp. 496-503 ◽  
Author(s):  
P A D’Amore ◽  
H B Hechtman ◽  
D Shepro

SummaryOrnithine decarboxylase (ODC) activity, the rate-limiting step in the synthesis of polyamines, can be demonstrated in cultured, bovine, aortic endothelial cells (EC). Serum, serotonin and thrombin produce a rise in ODC activity. The serotonin-induced ODC activity is significantly blocked by imipramine (10-5 M) or Lilly 11 0140 (10-6M). Preincubation of EC with these blockers together almost completely depresses the 5-HT-stimulated ODC activity. These observations suggest a manner by which platelets may maintain EC structural and metabolic soundness.


Diabetes ◽  
1993 ◽  
Vol 42 (2) ◽  
pp. 296-306 ◽  
Author(s):  
D. C. Bradley ◽  
R. A. Poulin ◽  
R. N. Bergman

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