Effective extracellular production ofBacillus stearothermophilus esterase by pH-stat modal fed-batch culture of recombinantBacillus brevis

1992 ◽  
Vol 40 (7) ◽  
pp. 844-850 ◽  
Author(s):  
Edgardo E. Tulin ◽  
Shunsaku Ueda ◽  
Hideo Yamagata ◽  
Shigezo Udaka ◽  
Tsuneo Yamane
2004 ◽  
Vol 98 (4) ◽  
pp. 263-268 ◽  
Author(s):  
Yukihiro Tashiro ◽  
Katsuhisa Takeda ◽  
Genta Kobayashi ◽  
Kenji Sonomoto ◽  
Ayaaki Ishizaki ◽  
...  

2000 ◽  
Vol 66 (8) ◽  
pp. 3624-3627 ◽  
Author(s):  
Woo Suk Ahn ◽  
Si Jae Park ◽  
Sang Yup Lee

ABSTRACT Fermentation strategies for the production of poly(3-hydroxybutyrate) (PHB) from whey by recombinantEscherichia coli strain CGSC 4401 harboring theAlcaligenes latus polyhydroxyalkanoate (PHA) biosynthesis genes were developed. The pH-stat fed-batch cultures of E. coli CGSC 4401 harboring pJC4, a stable plasmid containing theA. latus PHA biosynthesis genes, were carried out with a concentrated whey solution containing 280 g of lactose equivalent per liter. Final cell and PHB concentrations of 119.5 and 96.2 g/liter, respectively, were obtained in 37.5 h, which resulted in PHB productivity of 2.57 g/liter/h.


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