scholarly journals Incorporating physiologically relevant mobile phases in micellar liquid chromatography for the prediction of human intestinal absorption

2018 ◽  
Vol 32 (12) ◽  
pp. e4351 ◽  
Author(s):  
Dina S. Shokry ◽  
Laura J. Waters ◽  
Gareth M. B. Parkes ◽  
John C. Mitchell
Keyword(s):  
Liquid Chromatography ◽  
Intestinal Absorption ◽  
Micellar Liquid Chromatography ◽  
Human Intestinal Absorption ◽  
Mobile Phases

scholarly journals Predicting human intestinal absorption in the presence of bile salt with micellar liquid chromatography

2016 ◽  
Vol 30 (10) ◽  
pp. 1618-1624 ◽  
Author(s):  
Laura J. Waters ◽  
Dina S. Shokry ◽  
Gareth M.B. Parkes
Keyword(s):  
Liquid Chromatography ◽  
Bile Salt ◽  
Intestinal Absorption ◽  
Micellar Liquid Chromatography ◽  
Human Intestinal Absorption

scholarly journals Prediction of human intestinal absorption using micellar liquid chromatography with an aminopropyl stationary phase

2019 ◽  
Vol 33 (7) ◽  
pp. e4515
Author(s):  
Dina S. Shokry ◽  
Laura J. Waters ◽  
Gareth M.B. Parkes ◽  
John C. Mitchell
Keyword(s):  
Liquid Chromatography ◽  
Stationary Phase ◽  
Intestinal Absorption ◽  
Micellar Liquid Chromatography ◽  
Human Intestinal Absorption

scholarly journals In vitro prediction of human intestinal absorption and blood–brain barrier partitioning: development of a lipid analog for micellar liquid chromatography

2015 ◽  
Vol 407 (24) ◽  
pp. 7453-7466 ◽  
Author(s):  
Mike De Vrieze ◽  
Pieter Janssens ◽  
Roman Szucs ◽  
Johan Van der Eycken ◽  
Frédéric Lynen
Keyword(s):  
Liquid Chromatography ◽  
Intestinal Absorption ◽  
Blood Brain Barrier ◽  
Micellar Liquid Chromatography ◽  
Brain Barrier ◽  
Human Intestinal Absorption ◽  
Blood Brain

scholarly journals Analysis of Some Biogenic Amines by Micellar Liquid Chromatography

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Irena Malinowska ◽  
Katarzyna E. Stępnik
Keyword(s):  
Liquid Chromatography ◽  
Sodium Dodecyl Sulfate ◽  
Biogenic Amines ◽  
Physicochemical Parameters ◽  
Mobile Phase ◽  
Sodium Dodecyl ◽  
Micellar Liquid Chromatography ◽  
Ionic Surfactant ◽  
Mobile Phases ◽  
Dodecyl Sulfate

Micellar liquid chromatography (MLC) with the use of high performance liquid chromatography (HPLC) was used to determine some physicochemical parameters of six biogenic amines: adrenaline, dopamine, octopamine, histamine, 2-phenylethylamine, and tyramine. In this paper, an influence of surfactant’s concentration and pH of the micellar mobile phase on the retention of the tested substances was examined. To determine the influence of surfactant’s concentration on the retention of the tested amines, buffered solutions (at pH 7.4) of ionic surfactant—sodium dodecyl sulfate SDS (at different concentrations) with acetonitrile as an organic modifier (0.8/0.2 v/v) were used as the micellar mobile phases. To determine the influence of pH of the micellar mobile phase on the retention, mobile phases contained buffered solutions (at different pH values) of sodium dodecyl sulfate SDS (at 0.1 M) with acetonitrile (0.8/0.2 v/v). The inverse of value of retention factor () versus concentration of micelles () relationships were examined. Other physicochemical parameters of solutes such as an association constant analyte—micelle ()—and partition coefficient of analyte between stationary phase and water (hydrophobicity descriptor) () were determined by the use of Foley’s equation.

scholarly journals Retention Behaviour in Micellar Liquid Chromatography

2012 ◽  
Vol 2012 ◽  
pp. 1-5
Author(s):  
Maria Rambla-Alegre
Keyword(s):  
Liquid Chromatography ◽  
Phase Composition ◽  
Mobile Phase ◽  
A Priori ◽  
Great Accuracy ◽  
Micellar Liquid Chromatography ◽  
Computer Assisted ◽  
Mobile Phase Composition ◽  
Mobile Phases ◽  
Sequential Strategy

Retention in micellar liquid chromatography is highly reproducible and can be modelled using empirical or mechanistic models with great accuracy to predict the retention changes when the mobile phase composition varies (surfactant and organic solvent concentrations), thus facilitating the optimisation of separation conditions. In addition, the different equilibria inside the column among the solute, the mobile phase, and the modified stationary phase by monomers of surfactant have been exhaustively studied. In a sequential strategy, the retention of the solutes is not known a priori, and each set of mobile phases is designed by taking into account the retention observed with previous eluents. By contrast, in an interpretative strategy, the experiments are designed before the optimization process and used to fit a model that will allow the prediction of the retention of each solute. This strategy is more efficient and reliable. The sequential strategy will be inadequate when several local and/or secondary maxima exist, as frequently occurs in chromatography, and may not give the best maximum, that is to say, the optimum. More often than not, the complexity of the mixtures of compounds studied and the relevant modification of their chromatographic behaviour when changing the mobile phase composition requires the use of computer-assisted simulations in MLC to follow the modifications in the chromatograms in detail. These simulations can be done with sound reliability thanks to the use of chemometrics tools.

scholarly journals Studying the suitability of hybrid micelle liquid chromatography for estimating the lipophilicity of some partial dopamine agonists used to attain the reward circuit

2021 ◽  
Vol 8 (5) ◽  
pp. 202371
Author(s):  
M. E. K. Wahba ◽  
D. El Wasseef ◽  
D. El Sherbiny
Keyword(s):  
Liquid Chromatography ◽  
Dopamine Agonists ◽  
The United States ◽  
Micellar Liquid Chromatography ◽  
Retention Mechanism ◽  
Chromatographic Technique ◽  
Column Temperature ◽  
Mobile Phases ◽  
Abused Drugs

Three micellar-based mobile phases were developed and optimized for the simultaneous determination of certain partial dopamine agonists that are used to overcome the withdrawal symptoms of abused drugs, namely aripiprazole, pramipexole and piribedil. The studied drugs were separated using micellar liquid chromatography, hybrid micellar liquid chromatography (HMLC) and microemulsion liquid chromatography (MELC). The three developed mobile phases were studied to estimate their suitability for the measurement of log p -values of the studied drugs. Experimental determination of log P m/w values using the three mobile phases demonstrates that HMLC is the mobile phase of choice since the obtained practical log P m/w values were in accordance with the reported log P values, and calculated log P and log D values. An explanation of the obtained results was presented based on the separation retention mechanism for each chromatographic technique. Furthermore, the effect of the pH and the column temperature in HMLC on the practical log P m/w values was studied. To verify its suitability for experimental measurement of log P m/w  , HMLC was subjected to full validation according to the United States Pharmacopeia.

scholarly journals Basic Principles of MLC

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Maria Rambla-Alegre
Keyword(s):  
Liquid Chromatography ◽  
Mobile Phase ◽  
Reversed Phase ◽  
Micellar Liquid Chromatography ◽  
Basic Principles ◽  
Organic Mixtures ◽  
Mobile Phases ◽  
Efficient Alternative ◽  
Phase Liquid ◽  
Low Toxicity

Micellar liquid chromatography (MLC) is an efficient alternative to conventional reversed-phase liquid chromatography with hydro-organic mobile phases. Almost three decades of experience have resulted in an increasing production of analytical applications. Current concern about the environment also reveals MLC as an interesting technique for “green” chemistry because it uses mobile phases containing 90% or more water. These micellar mobile phases have a low toxicity and are not producing hazardous wastes. The stationary phase is modified with an approximately constant amount of surfactant monomers, and the solubilising capability of the mobile phase is altered by the presence of micelles, giving rise to a great variety of interactions (hydrophobic, ionic, and steric) with major implications in retention and selectivity. From its beginnings in 1980, the technique has evolved up to becoming in a real alternative in some instances (and a complement in others) to classical RPLC with aqueous-organic mixtures, owing to its peculiar features and unique advantages. The addition of an organic solvent to the mobile phase was, however, soon suggested in order to enhance the low efficiencies and weak elution strength associated with the mobile phases that contained only micelles.

scholarly journals Development of Micellar HPLC-UV Method for Determination of Pharmaceuticals in Water Samples

2018 ◽  
Vol 2018 ◽  
pp. 1-12 ◽  
Author(s):  
Danielle Cristina da Silva ◽  
Cláudio Celestino Oliveira
Keyword(s):  
Liquid Chromatography ◽  
Mobile Phase ◽  
Solid Phase ◽  
Sodium Dodecyl ◽  
Micellar Liquid Chromatography ◽  
Phase Extraction ◽  
Mobile Phases ◽  
Concentration Factors ◽  
Better Than

Method for extraction and determination of amoxicillin, caffeine, ciprofloxacin, norfloxacin, tetracycline, diclofenac, ibuprofen, nimesulide, levonorgestrel, and 17α-ethynylestradiol exploiting micellar liquid chromatography with PDA detector and solid-phase extraction was proposed. The usage of toxic solvents was low; the chromatographic separation of the medicaments was performed using a C18 column and mobile phases A and B containing 15.0% (v/v) ethanol, 3.0% (m/v) sodium dodecyl sulfate (SDS), and 0.02 mol·L−1 phosphate at pHs 7.0 and 8.0, respectively. The method is simple, selective, and fast, and the analytes were separated in 23.0 min. For extraction, 1000 mL of sample containing 2.0% (v/v) ethanol and 0.002 mol·L−1 citric acid at pH 2.50 was loaded through a 1000 mg of C18 cartridge. The analytes were eluted using 3.0 mL of ethanol, which were evaporated and redissolved in 0.5 mL of mobile phase. Concentration factors better than 1200, except amoxicillin (224), were obtained. The analytical curves were linear (R2 better than 0.992); LOD and LOQ n=10 presented values in the range of 0.019–0.247 and 0.058–0.752 mg·L−1, respectively. Recoveries of 99% were obtained, and the results are in agreement with those obtained by the comparative methods.

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