Screening, Molecular Cloning, and Biochemical Characterization of an Alcohol Dehydrogenase fromPichia pastorisUseful for the Kinetic Resolution of a Racemic β-Hydroxy-β-trifluoromethyl Ketone

ChemBioChem ◽  
2016 ◽  
Vol 17 (14) ◽  
pp. 1349-1358 ◽  
Author(s):  
Dalia Bulut ◽  
Nongnaphat Duangdee ◽  
Harald Gröger ◽  
Albrecht Berkessel ◽  
Werner Hummel
Keyword(s):  
Alcohol Dehydrogenase ◽  
Molecular Cloning ◽  
Kinetic Resolution ◽  
Biochemical Characterization

scholarly journals Genetic and biochemical characterization of a short-chain alcohol dehydrogenase from the hyperthermophilic archaeon Pyrococcus furiosu s

2001 ◽  
Vol 268 (10) ◽  
pp. 3062-3068 ◽  
Author(s):  
John van der Oost ◽  
Wilfried G. B. Voorhorst ◽  
Servé W. M. Kengen ◽  
Ans C. M. Geerling ◽  
Vincent Wittenhorst ◽  
...  
Keyword(s):  
Alcohol Dehydrogenase ◽  
Biochemical Characterization ◽  
Short Chain ◽  
Chain Alcohol ◽  
Hyperthermophilic Archaeon ◽  
Short Chain Alcohol

scholarly journals An Alcohol Dehydrogenase 3 (ADH3) from Entamoeba histolytica Is Involved in the Detoxification of Toxic Aldehydes

2020 ◽  
Vol 8 (10) ◽  
pp. 1608
Author(s):  
Constantin König ◽  
Martin Meyer ◽  
Corinna Lender ◽  
Sarah Nehls ◽  
Tina Wallaschkowski ◽  
...  
Keyword(s):  
Alcohol Dehydrogenase ◽  
Entamoeba Histolytica ◽  
Biochemical Characterization ◽  
Stop Codon ◽  
In Silico Analysis ◽  
Peptidase Activity ◽  
Stool Samples ◽  
Human Stool

Recently, a putative alcohol dehydrogenase 3, termed EhADH3B of the Entamoeba histolytica isolate HM-1:IMSS was identified, which is expressed at higher levels in non-pathogenic than in pathogenic amoebae and whose overexpression reduces the virulence of pathogenic amoebae. In an in silico analysis performed in this study, we assigned EhADH3B to a four-member ADH3 family, with ehadh3b present as a duplicate (ehadh3ba/ehadh3bb). In long-term laboratory cultures a mutation was identified at position 496 of ehadh3ba, which codes for a stop codon, which was not the case for amoebae isolated from human stool samples. When using transfectants that overexpress or silence ehadh3bb, we found no or little effect on growth, size, erythrophagocytosis, motility, hemolytic or cysteine peptidase activity. Biochemical characterization of the recombinant EhADH3Bb revealed that this protein forms a dimer containing Ni2+ or Zn2+ as a co-factor and that the enzyme converts acetaldehyde and formaldehyde in the presence of NADPH. A catalytic activity based on alcohols as substrates was not detected. Based on the results, we postulate that EhADH3Bb can reduce free acetaldehyde released by hydrolysis from bifunctional acetaldehyde/alcohol dehydrogenase-bound thiohemiacetal and that it is involved in detoxification of toxic aldehydes produced by the host or the gut microbiota.

Molecular cloning and biochemical characterization of a Tau class glutathione S-transferase from Pinus brutia Ten

Trees ◽  
2020 ◽  
Vol 34 (3) ◽  
pp. 835-843
Author(s):  
Meltem Alper ◽  
Elif Öztetik ◽  
M. Yaşar Kaya ◽  
Feray Köçkar
Keyword(s):  
Molecular Cloning ◽  
Biochemical Characterization ◽  
Glutathione S Transferase ◽  
Pinus Brutia

Gene Cloning and Biochemical Characterization of an Alcohol Dehydrogenase fromEuglena gracilis

2008 ◽  
Vol 55 (6) ◽  
pp. 554-561 ◽  
Author(s):  
HELGA N. PALMA-GUTIÉRREZ ◽  
JOSÉ SALUD RODRÍGUEZ-ZAVALA ◽  
RICARDO JASSO-CHÁVEZ ◽  
RAFAEL MORENO-SÁNCHEZ ◽  
EMMA SAAVEDRA
Keyword(s):  
Alcohol Dehydrogenase ◽  
Gene Cloning ◽  
Biochemical Characterization

Molecular cloning and biochemical characterization of a receptor-like serine/threonine kinase from rice

1994 ◽  
Vol 26 (3) ◽  
pp. 791-803 ◽  
Author(s):  
Yan Zhao ◽  
Xin-Hua Feng ◽  
John C. Watson ◽  
Paul J. Bottino ◽  
Shain-Dow Kung
Keyword(s):  
Molecular Cloning ◽  
Biochemical Characterization ◽  
Serine Threonine Kinase ◽  
Threonine Kinase

Molecular Cloning and Biochemical Characterization of Iron Superoxide Dismutase from Leishmania braziliensis

2018 ◽  
Vol 60 (8) ◽  
pp. 595-600
Author(s):  
Camila C. B. Brito ◽  
Fernando V. Maluf ◽  
Gustavo M. A. de Lima ◽  
Rafael V. C. Guido ◽  
Marcelo S. Castilho
Keyword(s):  
Superoxide Dismutase ◽  
Molecular Cloning ◽  
Biochemical Characterization ◽  
Leishmania Braziliensis ◽  
Iron Superoxide Dismutase

scholarly journals Molecular cloning, overexpression and biochemical characterization of hypothetical β-lactamases ofMycobacterium tuberculosisH37Rv

2008 ◽  
Vol 105 (1) ◽  
pp. 59-67 ◽  
Author(s):  
K.M. Nampoothiri ◽  
R. Rubex ◽  
A.K. Patel ◽  
S.S. Narayanan ◽  
S. Krishna ◽  
...  
Keyword(s):  
Molecular Cloning ◽  
Biochemical Characterization
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