ChemInform Abstract: Design, Synthesis and Evaluation of New α-Nucleophiles for the Hydrolysis of Organophosphorus Nerve Agents: Application to the Reactivation of Phosphorylated Acetylcholinesterase.

ChemInform ◽  
2011 ◽  
Vol 42 (52) ◽  
pp. no-no
Author(s):  
Geraldine Saint-Andre ◽  
Maria Kliachyna ◽  
Sanjeevarao Kodepelly ◽  
Ludivine Louise-Leriche ◽  
Emilie Gillon ◽  
...  
Keyword(s):  
Nerve Agents ◽  
Design Synthesis ◽  
Organophosphorus Nerve Agents ◽  
Hydrolysis Of

Design, synthesis and evaluation of new α-nucleophiles for the hydrolysis of organophosphorus nerve agents: application to the reactivation of phosphorylated acetylcholinesterase

Tetrahedron ◽  
2011 ◽  
Vol 67 (34) ◽  
pp. 6352-6361 ◽  
Author(s):  
Géraldine Saint-André ◽  
Maria Kliachyna ◽  
Sanjeevarao Kodepelly ◽  
Ludivine Louise-Leriche ◽  
Emilie Gillon ◽  
...  
Keyword(s):  
Nerve Agents ◽  
Design Synthesis ◽  
Organophosphorus Nerve Agents ◽  
Hydrolysis Of

Zirconium-Based Metal–Organic Frameworks for the Catalytic Hydrolysis of Organophosphorus Nerve Agents

2020 ◽  
Vol 12 (13) ◽  
pp. 14702-14720 ◽  
Author(s):  
Kent O. Kirlikovali ◽  
Zhijie Chen ◽  
Timur Islamoglu ◽  
Joseph T. Hupp ◽  
Omar K. Farha
Keyword(s):  
Metal Organic Frameworks ◽  
Nerve Agents ◽  
Catalytic Hydrolysis ◽  
Organophosphorus Nerve Agents ◽  
Metal Organic ◽  
Hydrolysis Of

Design and synthesis of haptens for antibody catalyzed hydrolysis of organophosphorus nerve agents

1999 ◽  
Vol 40 (2) ◽  
pp. 281-284 ◽  
Author(s):  
Pierre-Yves Renard ◽  
Philippe Vayron ◽  
Frédéric Taran ◽  
Charles Mioskowski
Keyword(s):  
Nerve Agents ◽  
Design And Synthesis ◽  
Organophosphorus Nerve Agents ◽  
Hydrolysis Of

Catalytic Detoxification of Organophosphorus Nerve Agents by Butyrylcholinesterase-Polymer-Oxime Bioscavengers

2020 ◽  
Vol 21 (9) ◽  
pp. 3867-3877
Author(s):  
Libin Zhang ◽  
Hironobu Murata ◽  
Gabriel Amitai ◽  
Paige N. Smith ◽  
Krzysztof Matyjaszewski ◽  
...  
Keyword(s):  
Nerve Agents ◽  
Organophosphorus Nerve Agents

scholarly journals Catalytic activity and stereoselectivity of engineered phosphotriesterases towards structurally different nerve agents in vitro

2021 ◽  
Author(s):  
Anja Köhler ◽  
Benjamin Escher ◽  
Laura Job ◽  
Marianne Koller ◽  
Horst Thiermann ◽  
...  
Keyword(s):  
Plasma Clearance ◽  
Nerve Agents ◽  
Inhibition Assay ◽  
Ache Inhibition ◽  
Catalytic Activities ◽  
Chiral Lc ◽  
Hydrolysis Of ◽  
Medical Countermeasures

AbstractHighly toxic organophosphorus nerve agents, especially the extremely stable and persistent V-type agents such as VX, still pose a threat to the human population and require effective medical countermeasures. Engineered mutants of the Brevundimonas diminuta phosphotriesterase (BdPTE) exhibit enhanced catalytic activities and have demonstrated detoxification in animal models, however, substrate specificity and fast plasma clearance limit their medical applicability. To allow better assessment of their substrate profiles, we have thoroughly investigated the catalytic efficacies of five BdPTE mutants with 17 different nerve agents using an AChE inhibition assay. In addition, we studied one BdPTE version that was fused with structurally disordered PAS polypeptides to enable delayed plasma clearance and one bispecific BdPTE with broadened substrate spectrum composed of two functionally distinct subunits connected by a PAS linker. Measured kcat/KM values were as high as 6.5 and 1.5 × 108 M−1 min−1 with G- and V-agents, respectively. Furthermore, the stereoselective degradation of VX enantiomers by the PASylated BdPTE-4 and the bispecific BdPTE-7 were investigated by chiral LC–MS/MS, resulting in a several fold faster hydrolysis of the more toxic P(−) VX stereoisomer compared to P(+) VX. In conclusion, the newly developed enzymes BdPTE-4 and BdPTE-7 have shown high catalytic efficacy towards structurally different nerve agents and stereoselectivity towards the toxic P(−) VX enantiomer in vitro and offer promise for use as bioscavengers in vivo.

Autocatalytic Hydrolysis of V-Type Nerve Agents

1996 ◽  
Vol 61 (24) ◽  
pp. 8407-8413 ◽  
Author(s):  
Yu-Chu Yang ◽  
Linda L. Szafraniec ◽  
William T. Beaudry ◽  
Dennis K. Rohrbaugh ◽  
Lawrence R. Procell ◽  
...  
Keyword(s):  
Nerve Agents ◽  
Hydrolysis Of
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