Kontinuierliche Selektivoxidation mittels Methylosinus trichosporium OB3b am Beispiel von Benzol und Propylen

2020 ◽  
Vol 92 (9) ◽  
pp. 1216-1217
Author(s):  
I. M. Mühlemeier ◽  
M. Stier ◽  
G. Tovar ◽  
T. Hirth
Keyword(s):  
Methylosinus Trichosporium Ob3b ◽  
Methylosinus Trichosporium

scholarly journals Methanobactin from Methylocystis sp. Strain SB2 Affects Gene Expression and Methane Monooxygenase Activity in Methylosinus trichosporium OB3b

2015 ◽  
Vol 81 (7) ◽  
pp. 2466-2473 ◽  
Author(s):  
Muhammad Farhan Ul-Haque ◽  
Bhagyalakshmi Kalidass ◽  
Alexey Vorobev ◽  
Bipin S. Baral ◽  
Alan A. DiSpirito ◽  
...  
Keyword(s):  
Gene Expression ◽  
Methane Monooxygenase ◽  
Particulate Methane Monooxygenase ◽  
Methylosinus Trichosporium Ob3b ◽  
Methylosinus Trichosporium ◽  
Monooxygenase Activity ◽  
Content Type ◽  
Soluble Methane Monooxygenase ◽  
A Subunit ◽  
Membrane Bound

ABSTRACTMethanotrophs can express a cytoplasmic (soluble) methane monooxygenase (sMMO) or membrane-bound (particulate) methane monooxygenase (pMMO). Expression of these MMOs is strongly regulated by the availability of copper. Many methanotrophs have been found to synthesize a novel compound, methanobactin (Mb), that is responsible for the uptake of copper, and methanobactin produced byMethylosinus trichosporiumOB3b plays a key role in controlling expression of MMO genes in this strain. As all known forms of methanobactin are structurally similar, it was hypothesized that methanobactin from one methanotroph may alter gene expression in another. WhenMethylosinus trichosporiumOB3b was grown in the presence of 1 μM CuCl2, expression ofmmoX, encoding a subunit of the hydroxylase component of sMMO, was very low.mmoXexpression increased, however, when methanobactin fromMethylocystissp. strain SB2 (SB2-Mb) was added, as did whole-cell sMMO activity, but there was no significant change in the amount of copper associated withM. trichosporiumOB3b. IfM. trichosporiumOB3b was grown in the absence of CuCl2, themmoXexpression level was high but decreased by several orders of magnitude if copper prebound to SB2-Mb (Cu-SB2-Mb) was added, and biomass-associated copper was increased. Exposure ofMethylosinus trichosporiumOB3b to SB2-Mb had no effect on expression ofmbnA, encoding the polypeptide precursor of methanobactin in either the presence or absence of CuCl2.mbnAexpression, however, was reduced when Cu-SB2-Mb was added in both the absence and presence of CuCl2. These data suggest that methanobactin acts as a general signaling molecule in methanotrophs and that methanobactin “piracy” may be commonplace.

Molecular analysis of the methane monooxygenase (MMO) gene cluster of Methylosinus trichosporium OB3b

1991 ◽  
Vol 5 (2) ◽  
pp. 335-342 ◽  
Author(s):  
D. L. N. Cardy ◽  
V. Laidler ◽  
G. P. C. Salmond ◽  
J. C. Murrell
Keyword(s):  
Gene Cluster ◽  
Molecular Analysis ◽  
Methane Monooxygenase ◽  
Methylosinus Trichosporium Ob3b ◽  
Methylosinus Trichosporium

The Ethylmalonyl-CoA Pathway for Methane-based Biorefineries: A Case Study of Using Methylosinus trichosporium OB3b, an Alpha-proteobacterial Methanotroph, for Producing 2-Hydroxyisobutyric Acid and 1,3-Butanediol from Methane

2021 ◽  
Author(s):  
Dung Hoang Anh Mai ◽  
Thu Thi Nguyen ◽  
Eun Yeol Lee
Keyword(s):  
Carbon Metabolism ◽  
Tricarboxylic Acid Cycle ◽  
Tricarboxylic Acid ◽  
Methylosinus Trichosporium Ob3b ◽  
Methylosinus Trichosporium ◽  
Acid Cycle ◽  
Hydroxyisobutyric Acid ◽  
Tricarboxylic Acid Cycle Intermediates ◽  
Alpha Proteobacteria

The ethylmalonyl-CoA pathway is one of three known anaplerotic pathways that replenish tricarboxylic acid cycle intermediates and plays a major role in the carbon metabolism of many alpha-proteobacteria including Methylosinus...

Enhanced mass transfer rate of methane via hollow fiber membrane modules for Methylosinus trichosporium OB3b fermentation

2016 ◽  
Vol 39 ◽  
pp. 149-152 ◽  
Author(s):  
Jaewon Lee ◽  
Nulee Jang ◽  
Muhammad Yasin ◽  
Eun Yeol Lee ◽  
In Seop Chang ◽  
...  
Keyword(s):  
Mass Transfer ◽  
Hollow Fiber ◽  
Transfer Rate ◽  
Hollow Fiber Membrane ◽  
Mass Transfer Rate ◽  
Fiber Membrane ◽  
Methylosinus Trichosporium Ob3b ◽  
Methylosinus Trichosporium ◽  
Membrane Modules

Spectral and thermodynamic properties of Ag(I), Au(III), Cd(II), Co(II), Fe(III), Hg(II), Mn(II), Ni(II), Pb(II), U(IV), and Zn(II) binding by methanobactin from Methylosinus trichosporium OB3b

2006 ◽  
Vol 100 (12) ◽  
pp. 2150-2161 ◽  
Author(s):  
Dong W. Choi ◽  
Young S. Do ◽  
Corbin J. Zea ◽  
Marcus T. McEllistrem ◽  
Sung-W. Lee ◽  
...  
Keyword(s):  
Thermodynamic Properties ◽  
Methylosinus Trichosporium Ob3b ◽  
Methylosinus Trichosporium

Copper-Binding Properties and Structures of Methanobactins from Methylosinus trichosporium OB3b

2011 ◽  
Vol 50 (4) ◽  
pp. 1378-1391 ◽  
Author(s):  
Abdelnasser El Ghazouani ◽  
Arnaud Baslé ◽  
Susan J. Firbank ◽  
Charles W. Knapp ◽  
Joe Gray ◽  
...  
Keyword(s):  
Copper Binding ◽  
Methylosinus Trichosporium Ob3b ◽  
Binding Properties ◽  
Methylosinus Trichosporium
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