Immunocytochemical localization of luteinizing hormone-releasing hormone (LHRH) in the brain and nervus terminalis of the adult and early neonatal gray short-tailed opossum (Monodelphis domestica)

1988 ◽  
Vol 276 (1) ◽  
pp. 44-60 ◽  
Author(s):  
Marlene Schwanzel Fukuda ◽  
Barbara H. Fadem ◽  
Maria Soledad Garcia ◽  
Donald W. Pfaff
Keyword(s):  
Luteinizing Hormone ◽  
Monodelphis Domestica ◽  
Immunocytochemical Localization ◽  
Luteinizing Hormone Releasing Hormone ◽  
Nervus Terminalis ◽  
Releasing Hormone ◽  
The Brain

scholarly journals Immunocytochemical localization of luteinizing hormone-releasing hormone (LHRH) and LHRH-like material in brain. Influence of fixation solution at various pH values.

1983 ◽  
Vol 31 (11) ◽  
pp. 1329-1332 ◽  
Author(s):  
D T Piekut
Keyword(s):  
Luteinizing Hormone ◽  
Pineal Gland ◽  
Ph Value ◽  
Acidic Ph ◽  
Immunocytochemical Localization ◽  
Luteinizing Hormone Releasing Hormone ◽  
Releasing Hormone ◽  
Ph Values ◽  
Rat Pineal Gland ◽  
The Brain

The amount and distribution of luteinizing hormone-releasing hormone (LHRH) immunoreactive (ir) material in the hypothalamus, and of a LHRH-like immunostained substance in the rat pineal gland were examined employing Bouin's and Zamboni's fixation solutions at various pH values, and antisera generated against synthetic LHRH. The hypothalamic LHRH immunoreaction product is optimally visualized in this study when the pH value of the fixation solution is basic; conversely, the pineal LHRH-like ir material is seen only with an acidic pH value of the fixation solution. The present study demonstrates that the pH value of the fixation solution contributes substantially to the amount and distribution of some peptides visualized immunocytochemically in the brain.

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