Immunocytochemical localization of luteinizing hormone-releasing hormone in the brain of the goldfish Carassius auratus

1984 ◽  
Vol 53 (1) ◽  
pp. 107-115 ◽  
Author(s):  
Olivier Kah ◽  
Pascal Chambolle ◽  
Pierrette Dubourg ◽  
Maurice P. Dubois
Keyword(s):  
Luteinizing Hormone ◽  
Carassius Auratus ◽  
Immunocytochemical Localization ◽  
Luteinizing Hormone Releasing Hormone ◽  
Releasing Hormone ◽  
Goldfish Carassius Auratus ◽  
The Brain

scholarly journals Immunocytochemical localization of luteinizing hormone-releasing hormone (LHRH) and LHRH-like material in brain. Influence of fixation solution at various pH values.

1983 ◽  
Vol 31 (11) ◽  
pp. 1329-1332 ◽  
Author(s):  
D T Piekut
Keyword(s):  
Luteinizing Hormone ◽  
Pineal Gland ◽  
Ph Value ◽  
Acidic Ph ◽  
Immunocytochemical Localization ◽  
Luteinizing Hormone Releasing Hormone ◽  
Releasing Hormone ◽  
Ph Values ◽  
Rat Pineal Gland ◽  
The Brain

The amount and distribution of luteinizing hormone-releasing hormone (LHRH) immunoreactive (ir) material in the hypothalamus, and of a LHRH-like immunostained substance in the rat pineal gland were examined employing Bouin's and Zamboni's fixation solutions at various pH values, and antisera generated against synthetic LHRH. The hypothalamic LHRH immunoreaction product is optimally visualized in this study when the pH value of the fixation solution is basic; conversely, the pineal LHRH-like ir material is seen only with an acidic pH value of the fixation solution. The present study demonstrates that the pH value of the fixation solution contributes substantially to the amount and distribution of some peptides visualized immunocytochemically in the brain.

Comparison of [D-Arg6, Trp7, Leu8, Pro9NEt]-luteinizing hormone-releasing hormone (sGnRH-A), and [D-Ala6, Pro9NEt]-luteinizing hormone-releasing hormone (LHRH-A), in combination with pimozide, in stimulating gonadotropin release and ovulation in the goldfish, Carassius auratus

1987 ◽  
Vol 65 (4) ◽  
pp. 987-991 ◽  
Author(s):  
R. E. Peter ◽  
M. Sokolowska ◽  
C. S. Nahorniak ◽  
J. E. Rivier ◽  
W. W. Vale
Keyword(s):  
Luteinizing Hormone ◽  
Carassius Auratus ◽  
Luteinizing Hormone Releasing Hormone ◽  
Releasing Hormone ◽  
Gonadotropin Release ◽  
Goldfish Carassius Auratus ◽  
Highly Effective ◽  
Serum Gonadotropin ◽  
Low Dosage

LHRH-A and sGnRH-A were tested, in the presence or absence of pimozide (Pim), for their ability to stimulate increases in serum gonadotropin (GtH) levels and ovulation in prespawning female goldfish. In the absence of Pim, sGnRH-A was more active than LHRH-A in terms of stimulating serum GtH levels; neither peptide given alone was effective in stimulating ovulation. Pim potentiated the activity of both peptides; high dosages of either peptide, plus a high dosage of Pim, were highly effective in stimulating serum GtH and ovulation. A low dosage of sGnRH-A, plus a low dosage of Pim, were also effective in stimulating serum GtH and ovulation; however, a low dosage of LHRH-A plus a low dosage of Pim were ineffective. The time to ovulation following injections, for those treatments that were highly effective in inducing ovulation, was highly predictable, and oocyte fertility and viability were high. These results indicate that sGnRH-A, in the presence of Pim, is effective in stimulating ovulation in goldfish at dosages about 10-fold less than for LHRH-A. The basis for the differences in potency between sGnRH-A and LHRH-A is discussed.

scholarly journals Immunocytochemical localization of luteinizing hormone-releasing hormone (LHRH) in Vibratome-sectioned brain.

1981 ◽  
Vol 29 (2) ◽  
pp. 247-254 ◽  
Author(s):  
S A Joseph ◽  
D T Piekut ◽  
K M Knigge
Keyword(s):  
Luteinizing Hormone ◽  
Immunocytochemical Localization ◽  
Fiber Distribution ◽  
Fixed Tissue ◽  
Luteinizing Hormone Releasing Hormone ◽  
Final Reaction Product ◽  
Releasing Hormone ◽  
Tissue Sections ◽  
Dehydration Processes ◽  
Sectioned Tissue

Immunocytochemical localization of neuropeptides such as luteinizing hormone-releasing hormone (LHRH) is generally performed on Bouin's fixed tissue sections, following tissue dehydration in alcohols and embedment in paraffin. When the final reaction product accurately reflects content and distribution of the neuropeptide has not been examined carefully. Our data indicate a decrease in radioimmunoassayable LHRH content of brain fixed in Bouin's solution and a further significant reduction following dehydration with alcohol. In order to circumvent this loss of hormone, sections of fixed brain were cut on a Vibratome at 30 micron and collected in phosphate-saline buffer. These Vibratome sections revealed a significantly greater amount of overall immunoreactivity and fiber distribution as compared to paraffin-embedded sectioned tissue. These results suggest that certain neuropeptides, soluble in alcohol, can be extracted during dehydration processes necessary for paraffin embedment.

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