The Immunocytochemical Localization of Luteinizing Hormone-Releasing Hormone in the Brain of the Gray Short-Tailed Opossum (Monodelphis domestica)

1987 ◽  
Vol 519 (1 The Terminal) ◽  
pp. 213-228 ◽  
Author(s):  
MARLENE SCHWANZEL-FUKUDA ◽  
BARBARA H. FADEM ◽  
MARIA SOLEDAD GARCIA ◽  
DONALD W. PFAFF
Keyword(s):  
Luteinizing Hormone ◽  
Monodelphis Domestica ◽  
Immunocytochemical Localization ◽  
Luteinizing Hormone Releasing Hormone ◽  
Releasing Hormone ◽  
The Brain

scholarly journals Immunocytochemical localization of luteinizing hormone-releasing hormone (LHRH) and LHRH-like material in brain. Influence of fixation solution at various pH values.

1983 ◽  
Vol 31 (11) ◽  
pp. 1329-1332 ◽  
Author(s):  
D T Piekut
Keyword(s):  
Luteinizing Hormone ◽  
Pineal Gland ◽  
Ph Value ◽  
Acidic Ph ◽  
Immunocytochemical Localization ◽  
Luteinizing Hormone Releasing Hormone ◽  
Releasing Hormone ◽  
Ph Values ◽  
Rat Pineal Gland ◽  
The Brain

The amount and distribution of luteinizing hormone-releasing hormone (LHRH) immunoreactive (ir) material in the hypothalamus, and of a LHRH-like immunostained substance in the rat pineal gland were examined employing Bouin's and Zamboni's fixation solutions at various pH values, and antisera generated against synthetic LHRH. The hypothalamic LHRH immunoreaction product is optimally visualized in this study when the pH value of the fixation solution is basic; conversely, the pineal LHRH-like ir material is seen only with an acidic pH value of the fixation solution. The present study demonstrates that the pH value of the fixation solution contributes substantially to the amount and distribution of some peptides visualized immunocytochemically in the brain.

scholarly journals Immunocytochemical localization of luteinizing hormone-releasing hormone (LHRH) in Vibratome-sectioned brain.

1981 ◽  
Vol 29 (2) ◽  
pp. 247-254 ◽  
Author(s):  
S A Joseph ◽  
D T Piekut ◽  
K M Knigge
Keyword(s):  
Luteinizing Hormone ◽  
Immunocytochemical Localization ◽  
Fiber Distribution ◽  
Fixed Tissue ◽  
Luteinizing Hormone Releasing Hormone ◽  
Final Reaction Product ◽  
Releasing Hormone ◽  
Tissue Sections ◽  
Dehydration Processes ◽  
Sectioned Tissue

Immunocytochemical localization of neuropeptides such as luteinizing hormone-releasing hormone (LHRH) is generally performed on Bouin's fixed tissue sections, following tissue dehydration in alcohols and embedment in paraffin. When the final reaction product accurately reflects content and distribution of the neuropeptide has not been examined carefully. Our data indicate a decrease in radioimmunoassayable LHRH content of brain fixed in Bouin's solution and a further significant reduction following dehydration with alcohol. In order to circumvent this loss of hormone, sections of fixed brain were cut on a Vibratome at 30 micron and collected in phosphate-saline buffer. These Vibratome sections revealed a significantly greater amount of overall immunoreactivity and fiber distribution as compared to paraffin-embedded sectioned tissue. These results suggest that certain neuropeptides, soluble in alcohol, can be extracted during dehydration processes necessary for paraffin embedment.

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