13C-Detected Through-Bond Correlation Experiments for Protein Resonance Assignment by Ultra-Fast MAS Solid-State NMR

ChemPhysChem ◽  
2013 ◽  
Vol 14 (13) ◽  
pp. 3131-3137 ◽  
Author(s):  
Emeline Barbet-Massin ◽  
Andrew J. Pell ◽  
Michael J. Knight ◽  
Amy L. Webber ◽  
Isabella C. Felli ◽  
...  
Keyword(s):  
Solid State ◽  
Resonance Assignment ◽  
Solid State Nmr ◽  
Protein Resonance Assignment ◽  
Fast Mas

Protein resonance assignment by solid-state NMR based on 1H-detected 13C double-quantum spectroscopy at fast MAS

2021 ◽  
Author(s):  
Alons Lends ◽  
Mélanie Berbon ◽  
Birgit Habenstein ◽  
Yusuke Nishiyama ◽  
Antoine Loquet
Keyword(s):  
Solid State ◽  
Resonance Assignment ◽  
Solid State Nmr ◽  
Double Quantum ◽  
Protein Resonance Assignment ◽  
Fast Mas

Out-and-back 13C–13C scalar transfers in protein resonance assignment by proton-detected solid-state NMR under ultra-fast MAS

2013 ◽  
Vol 56 (4) ◽  
pp. 379-386 ◽  
Author(s):  
Emeline Barbet-Massin ◽  
Andrew J. Pell ◽  
Kristaps Jaudzems ◽  
W. Trent Franks ◽  
Joren S. Retel ◽  
...  
Keyword(s):  
Solid State ◽  
Resonance Assignment ◽  
Solid State Nmr ◽  
Protein Resonance Assignment ◽  
Fast Mas

scholarly journals Protein resonance assignment by BSH-CP-based 3D solid-state NMR experiments: A practical guide

2019 ◽  
Vol 58 (5) ◽  
pp. 445-465
Author(s):  
Jutta Hoffmann ◽  
Julia Ruta ◽  
Chaowei Shi ◽  
Kitty Hendriks ◽  
Veniamin Chevelkov ◽  
...  
Keyword(s):  
Solid State ◽  
Resonance Assignment ◽  
Solid State Nmr ◽  
Practical Guide ◽  
Protein Resonance Assignment ◽  
3D Solid

scholarly journals Protein resonance assignment by solid-state NMR based on 1H-detected 13C-based double-quantum spectroscopy at fast MAS

2021 ◽  
Author(s):  
Alons Lends ◽  
Melanie Berbon ◽  
Birgit Habenstein ◽  
Yusuke Nishiyama ◽  
Antoine Loquet
Keyword(s):  
Solid State ◽  
Resonance Assignment ◽  
Solid State Nmr ◽  
Protein Complexes ◽  
Three Dimensional ◽  
Magic Angle Spinning ◽  
Sequential Assignment ◽  
Double Quantum ◽  
Magic Angle ◽  
Fast Mas

Solid-state NMR spectroscopy is a powerful technique to study insoluble and non-crystalline proteins and protein complexes at atomic resolution. The development of proton (1H) detection at fast magic-angle spinning (MAS) has considerably increased the analytical capabilities of the technique, enabling the acquisition of 1H-detected fingerprint experiments in few hours. Here an approach based on double-quantum (DQ) 13C spectroscopy, detected on 1H, is introduced at fast MAS (70 kHz) to perform the sequential assignment of insoluble proteins of small size, without any specific deuteration requirement. By combining two three-dimensional 1H detected experiments correlating a 13C DQ dimension respectively to its intra-residue and sequential 15N-1H pairs, a sequential walk through DQ (CA+CO) resonance is obtained. Our approach takes advantage of fast MAS to achieve an efficient sensitivity and the addition of a DQ dimension provides spectral features useful for the resonance assignment process.

scholarly journals BSH-CP based 3D solid-state NMR experiments for protein resonance assignment

2014 ◽  
Vol 59 (1) ◽  
pp. 15-22 ◽  
Author(s):  
Chaowei Shi ◽  
Hannes K. Fasshuber ◽  
Veniamin Chevelkov ◽  
Shengqi Xiang ◽  
Birgit Habenstein ◽  
...  
Keyword(s):  
Solid State ◽  
Resonance Assignment ◽  
Solid State Nmr ◽  
Protein Resonance Assignment ◽  
3D Solid

Recent progress in dipolar recoupling techniques under fast MAS in solid-state NMR spectroscopy

2021 ◽  
Vol 112 ◽  
pp. 101711
Author(s):  
Yi Ji ◽  
Lixin Liang ◽  
Xinhe Bao ◽  
Guangjin Hou
Keyword(s):  
Solid State ◽  
Nmr Spectroscopy ◽  
Solid State Nmr ◽  
Recent Progress ◽  
Solid State Nmr Spectroscopy ◽  
Dipolar Recoupling ◽  
Fast Mas

Rapid Characterization of Formulated Pharmaceuticals Using Fast MAS 1H Solid-State NMR Spectroscopy

2019 ◽  
Vol 16 (7) ◽  
pp. 3121-3132 ◽  
Author(s):  
David A. Hirsh ◽  
Anuradha V. Wijesekara ◽  
Scott L. Carnahan ◽  
Ivan Hung ◽  
Joseph W. Lubach ◽  
...  
Keyword(s):  
Solid State ◽  
Nmr Spectroscopy ◽  
Solid State Nmr ◽  
Solid State Nmr Spectroscopy ◽  
Fast Mas ◽  
Rapid Characterization

scholarly journals Resonance assignment of the outer membrane protein AlkL in lipid bilayers by proton-detected solid-state NMR

2020 ◽  
Vol 14 (2) ◽  
pp. 295-300 ◽  
Author(s):  
Tobias Schubeis ◽  
Tom S. Schwarzer ◽  
Tanguy Le Marchand ◽  
Jan Stanek ◽  
Kumar Tekwani Movellan ◽  
...  
Keyword(s):  
Solid State ◽  
Membrane Protein ◽  
Outer Membrane ◽  
Lipid Bilayers ◽  
Resonance Assignment ◽  
Outer Membrane Protein ◽  
Solid State Nmr
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