Artificial Restriction DNA Cutter Using Nuclease S1 for Site‐Selective Scission of Genomic DNA

2019 ◽  
pp. e72 ◽  
Author(s):  
Arivazhagan Rajendran ◽  
Narumi Shigi ◽  
Jun Sumaoka ◽  
Makoto Komiyama
Keyword(s):  
Genomic Dna ◽  
Nuclease S1 ◽  
Artificial Restriction ◽  
Site Selective

Affinity Isolation of Defined Genomic Fragments Cleaved by Nuclease S1-based Artificial Restriction DNA Cutter

2019 ◽  
Vol 76 (1) ◽  
pp. e76 ◽  
Author(s):  
Arivazhagan Rajendran ◽  
Narumi Shigi ◽  
Jun Sumaoka ◽  
Makoto Komiyama
Keyword(s):  
Nuclease S1 ◽  
Affinity Isolation ◽  
Artificial Restriction

Artificial restriction DNA cutter for site-selective scission of double-stranded DNA with tunable scission site and specificity

2008 ◽  
Vol 3 (4) ◽  
pp. 655-662 ◽  
Author(s):  
Makoto Komiyama ◽  
Yuichiro Aiba ◽  
Yoji Yamamoto ◽  
Jun Sumaoka
Keyword(s):  
Double Stranded Dna ◽  
Artificial Restriction ◽  
Site Selective

scholarly journals Site-selective hydrolysis of huge DNA by artificial restriction DNA cutter

2006 ◽  
Vol 50 (1) ◽  
pp. 265-266
Author(s):  
Yoji Yamamoto ◽  
Kazuyuki Miura ◽  
Makoto Komiyama
Keyword(s):  
Selective Hydrolysis ◽  
Artificial Restriction ◽  
Site Selective ◽  
Hydrolysis Of

scholarly journals An artificial restriction DNA cutter for site-selective gene insertion in human cells

2013 ◽  
Vol 49 (60) ◽  
pp. 6764 ◽  
Author(s):  
Kenichiro Ito ◽  
Narumi Shigi ◽  
Makoto Komiyama
Keyword(s):  
Human Cells ◽  
Gene Insertion ◽  
Artificial Restriction ◽  
Site Selective

Site-selective scission of human genome by artificial restriction DNA cutter

2009 ◽  
pp. 6542 ◽  
Author(s):  
Kenichiro Ito ◽  
Hitoshi Katada ◽  
Narumi Shigi ◽  
Makoto Komiyama
Keyword(s):  
Human Genome ◽  
Artificial Restriction ◽  
Site Selective

Site-specific Scission of Lambda Phage Genomic DNA by Ce(IV)/EDTA-based Artificial Restriction DNA Cutter

2006 ◽  
Vol 35 (6) ◽  
pp. 594-595 ◽  
Author(s):  
Yoji Yamamoto ◽  
Kazuyuki Miura ◽  
Makoto Komiyama
Keyword(s):  
Genomic Dna ◽  
Lambda Phage ◽  
Site Specific ◽  
Artificial Restriction

Double-strand cleavage at a two-base deletion mismatch in a DNA heteroduplex by nuclease S1

1985 ◽  
Vol 5 (8) ◽  
pp. 627-632 ◽  
Author(s):  
Michael G. Burdon ◽  
Jennifer H. Lees
Keyword(s):  
Plasmid Dna ◽  
Genomic Dna ◽  
Linear Plasmid ◽  
Dna Molecules ◽  
Potential Value ◽  
Nuclease S1

A two-base deletion mismatch was generated in a DNA heteroduplex by hybridization of two linear plasmid DNA molecules differing only by the presence of a two-base deletion in one of them. The heteroduplex was shown to be sensitive to double-strand cleavage by nuclease S1, thus demonstrating the potential value of single-stranded probes for the detection of polymorphisms in genomic DNA due to very small deletions.

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