Isolation of Microsomal Membrane Proteins from Arabidopsis thaliana

2016 ◽  
Vol 1 (1) ◽  
pp. 217-234 ◽  
Author(s):  
Erica D. LaMontagne ◽  
Carina A. Collins ◽  
Scott C. Peck ◽  
Antje Heese
Keyword(s):  
Arabidopsis Thaliana ◽  
Membrane Proteins ◽  
Microsomal Membrane

scholarly journals Isolation and characterization of major intrinsic microsomal membrane proteins.

1976 ◽  
Vol 251 (23) ◽  
pp. 7699-7708
Author(s):  
G R Blackburn ◽  
C B Kasper
Keyword(s):  
Membrane Proteins ◽  
Microsomal Membrane ◽  
Isolation And Characterization

Thyroid microsomal membrane proteins

1975 ◽  
Vol 379 (1) ◽  
pp. 262-270 ◽  
Author(s):  
Joseph T. Neary ◽  
Ann Armstrong ◽  
Betty Davidson ◽  
Farahe Maloof ◽  
Morris Soodak
Keyword(s):  
Membrane Proteins ◽  
Microsomal Membrane

scholarly journals ON THE SPATIAL ARRANGEMENT OF PROTEINS IN MICROSOMAL MEMBRANES FROM RAT LIVER

1974 ◽  
Vol 60 (3) ◽  
pp. 616-627 ◽  
Author(s):  
Gert Kreibich ◽  
Ann L. Hubbard ◽  
David D. Sabatini
Keyword(s):  
Membrane Proteins ◽  
Rat Liver ◽  
Gel Electrophoresis ◽  
Pyridoxal Phosphate ◽  
Sodium Dodecyl ◽  
Spatial Arrangement ◽  
Microsomal Membrane ◽  
Small Molecular Weight ◽  
Low Concentrations ◽  
Microsomal Membranes

Rat liver rough microsomes were labeled enzymatically with 125I using lactoperoxidase and glucose oxidase. In intact microsomes only proteins exposed on the outside face of the microsomal membrane were iodinated. Low concentrations of detergent (0.049% deoxycholate) were used to allow entrance of the iodination system into the vesicles without disassembling the membranes. This led to iodination of the soluble content proteins and to an increased labeling of the membrane proteins. The distribution of radioactivity in microsomal proteins was analyzed after separation by sodium dodecyl sulfate acrylamide gel electrophoresis. Most membrane proteins were labeled when intact microsomes were iodinated. No major membrane proteins were exclusively labeled in the presence of low detergent concentrations or after complete membrane disassembly. Therefore it is unlikely that there are major membrane proteins, other than glycoproteins, present only on the inner membrane face or completely embedded within the microsomal membrane. Microsomal proteins were also labeled by incubating rough microsomes with [3H]-NaBH4 after reaction with pyridoxal phosphate. Microsomal membranes were permeable to these small molecular weight reagents as shown by the fact that proteins in the vesicular cavity as well as membrane proteins were labeled with this system.

Isolation and structural analysis of microsomal membrane proteins

10.2741/a295 ◽  
1998 ◽  
Vol 3 (4) ◽  
pp. d483-493 ◽  
Author(s):  
Juris Ozols
Keyword(s):  
Structural Analysis ◽  
Membrane Proteins ◽  
Microsomal Membrane

scholarly journals Reconstitution of an electrogenic auxin transport activity mediated by Arabidopsis thaliana plasma membrane proteins

FEBS Letters ◽  
1999 ◽  
Vol 446 (1) ◽  
pp. 153-156 ◽  
Author(s):  
Wojciech Szponarski ◽  
Olivier Guibal ◽  
Meriam Espuna ◽  
Patrick Doumas ◽  
Michel Rossignol ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Plasma Membrane ◽  
Membrane Proteins ◽  
Auxin Transport ◽  
Transport Activity ◽  
Plasma Membrane Proteins

Triton X-114 phase separation in the isolation and purification of mouse liver microsomal membrane proteins

Methods ◽  
2011 ◽  
Vol 54 (4) ◽  
pp. 396-406 ◽  
Author(s):  
Rommel A. Mathias ◽  
Yuan-Shou Chen ◽  
Eugene A. Kapp ◽  
David W. Greening ◽  
Suresh Mathivanan ◽  
...  
Keyword(s):  
Phase Separation ◽  
Membrane Proteins ◽  
Mouse Liver ◽  
Microsomal Membrane ◽  
Isolation And Purification ◽  
Triton X

Resolution of Microsomal Membrane Proteins by Two-Dimensional Gel Electrophoresis

1978 ◽  
Vol 6 (6) ◽  
pp. 1366-1369 ◽  
Author(s):  
MUSTAKALI KADERBHAI ◽  
ROBERT B. FREEDMAN
Keyword(s):  
Membrane Proteins ◽  
Gel Electrophoresis ◽  
Microsomal Membrane ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis

Effects of polyamines on in vitro phosphorylation of soluble and plasma membrane proteins in tobacco, cucumber and Arabidopsis thaliana

Plant Science ◽  
1994 ◽  
Vol 97 (2) ◽  
pp. 109-118 ◽  
Author(s):  
Xiang S. Ye ◽  
Sergei A. Avdiushko ◽  
Joseph Kuc
Keyword(s):  
Arabidopsis Thaliana ◽  
Plasma Membrane ◽  
Membrane Proteins ◽  
Plasma Membrane Proteins
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