scholarly journals Production of Structured RNA Fragments by In Vitro Transcription and HPLC Purification

2021 ◽  
Vol 1 (6) ◽  
Author(s):  
Hampus Karlsson ◽  
Hannes Feyrer ◽  
Lorenzo Baronti ◽  
Katja Petzold
Keyword(s):  
In Vitro Transcription ◽  
Hplc Purification ◽  
Rna Fragments

RNA design by in vitro RNA recombination and synthesis

1987 ◽  
Vol 65 (8) ◽  
pp. 677-692 ◽  
Author(s):  
Robert Cedergren ◽  
Henri Grosjean
Keyword(s):  
In Vitro Transcription ◽  
Biologically Active ◽  
Future Research ◽  
Rna Recombination ◽  
Rna Sequences ◽  
Active Length ◽  
Rna Fragments ◽  
Rna Design ◽  
Relationship Of

The techniques of in vitro RNA synthesis and recombination are presented. These include the site-specific cleavage of RNA, the manipulation of terminal phosphates, and the ligation of RNA fragments. Areas of promising future research include the establishment of RNA cloning vectors and the use of in vitro transcription of natural or designed RNA genes. The chemical synthesis approach now offers the possibility of making large amounts of biologically active length RNAs and of incorporating modified or reporter nucleotides into RNA sequences for physical studies. The new RNA techniques taken with DNA technology will permit a new approach towards understanding the complexity of RNA metabolism and the relationship of structure to function in RNAs.

In vitro transcription and translation of simian rotavirus SA11 gene products.

1980 ◽  
Vol 33 (3) ◽  
pp. 1111-1121 ◽  
Author(s):  
B B Mason ◽  
D Y Graham ◽  
M K Estes
Keyword(s):  
In Vitro Transcription ◽  
Gene Products
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