The glial cell’s role in antinociceptive differential effects of oxytocin upon female and male rats

2022 ◽  
Author(s):  
Ana B. Salinas‐Abarca ◽  
Francisco G. Vázquez‐Cuevas ◽  
Adriana González‐Gallardo ◽  
Guadalupe Martínez‐Lorenzana ◽  
Abimael González‐Hernández ◽  
...  
Keyword(s):  
Male Rats ◽  
Differential Effects

scholarly journals High-fat diet-induced and genetically inherited obesity differentially alters DNA methylation profile in the germline of adult male rats

2020 ◽  
Vol 12 (1) ◽  
Author(s):  
Sharvari S. Deshpande ◽  
Harishankar Nemani ◽  
Gandhimathi Arumugam ◽  
Avinash Ravichandran ◽  
Nafisa H. Balasinor
Keyword(s):  
Dna Methylation ◽  
Real Time ◽  
Real Time Pcr ◽  
High Fat Diet ◽  
Male Rats ◽  
Global Dna Methylation ◽  
High Fat ◽  
Differential Effects ◽  
Male Germline ◽  
Embryo Loss

Abstract Background Paternal obesity has been associated with reduced live birth rates. It could lead to inheritance of metabolic disturbances to the offspring through epigenetic mechanisms. However, obesity is a multifactorial disorder with genetic or environmental causes. Earlier we had demonstrated differential effects of high-fat diet-induced obesity (DIO) and genetically inherited obesity (GIO) on metabolic, hormonal profile, male fertility, and spermatogenesis using two rat models. The present study aimed to understand the effect of DIO and GIO on DNA methylation in male germline, and its subsequent effects on the resorbed (post-implantation embryo loss) and normal embryos. First, we assessed the DNA methylation enzymatic machinery in the testis by Real-Time PCR, followed global DNA methylation levels in spermatozoa and testicular cells by ELISA and flow cytometry, respectively. Further, we performed Methylation Sequencing in spermatozoa for both the groups. Sequencing data in spermatozoa from both the groups were validated using Pyrosequencing. Expression of the differentially methylated genes was assessed in the resorbed and normal embryos sired by the DIO group using Real-Time PCR for functional validation. Results We noted a significant decrease in Dnmt transcript and global DNA methylation levels in the DIO group and an increase in the GIO group. Sequencing analysis showed 16,966 and 9113 differentially methylated regions in the spermatozoa of the DIO and GIO groups, respectively. Upon pathway analysis, we observed genes enriched in pathways involved in embryo growth and development namely Wnt, Hedgehog, TGF-beta, and Notch in spermatozoa for both the groups, the methylation status of which partially correlated with the gene expression pattern in resorbed and normal embryos sired by the DIO group. Conclusion Our study reports the mechanism by which diet-induced and genetically inherited obesity causes differential effects on the DNA methylation in the male germline that could be due to a difference in the white adipose tissue accumulation. These differences could either lead to embryo loss or transmit obesity-related traits to the offspring in adult life.

Differential Effects of Cefmetazole Sodium on the Reproductive System of Infant and Pubertal Male Rats

1989 ◽  
Vol 13 (1) ◽  
pp. 146-155
Author(s):  
J. B. MOE ◽  
K. SOTANI ◽  
J. MANABE ◽  
N. IKEGAMI ◽  
H. TANASE ◽  
...  
Keyword(s):  
Reproductive System ◽  
Male Rats ◽  
Differential Effects

Differential effects of d-amphetamine on vigilance in younger and older male rats

1994 ◽  
Vol 49 (3) ◽  
pp. 497-502 ◽  
Author(s):  
David M. Grilly ◽  
Barbara B. Simon
Keyword(s):  
Male Rats ◽  
Differential Effects

scholarly journals Differential effects of stress on fear learning and activation of the amygdala in pre-adolescent and adult male rats

Neuroscience ◽  
2017 ◽  
Vol 360 ◽  
pp. 210-219 ◽  
Author(s):  
Georgia Barbayannis ◽  
Daly Franco ◽  
Solange Wong ◽  
Josselyn Galdamez ◽  
Russell D. Romeo ◽  
...  
Keyword(s):  
Adult Male ◽  
Fear Learning ◽  
Male Rats ◽  
Differential Effects ◽  
Effects Of Stress
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