A voltage gradient-induced arrest of circular DNA during agarose gel electrophoresis

1987 ◽  
Vol 8 (5) ◽  
pp. 244-246 ◽  
Author(s):  
Philip Serwer ◽  
Shirley J. Hayes
Keyword(s):  
Gel Electrophoresis ◽  
Voltage Gradient ◽  
Agarose Gel ◽  
Agarose Gel Electrophoresis ◽  
Circular Dna

Agarose gel electrophoresis of circular DNA of replicative form of bacteriophage G4

1977 ◽  
Vol 78 (1) ◽  
pp. 260-275 ◽  
Author(s):  
Frances C. Wheeler ◽  
Richard A. Fishel ◽  
Robert C. Warner
Keyword(s):  
Gel Electrophoresis ◽  
Agarose Gel ◽  
Agarose Gel Electrophoresis ◽  
Replicative Form ◽  
Circular Dna

scholarly journals Ligation and supercoiling of plasmids containing single strand interruptions

2021 ◽  
Author(s):  
Felipe A. Calil ◽  
Christopher D. Putnam ◽  
Richard D. Kolodner
Keyword(s):  
Gel Electrophoresis ◽  
Simple Procedure ◽  
Dna Gyrase ◽  
Single Strand ◽  
Dna Ligase ◽  
Hydroxyl Groups ◽  
Agarose Gel ◽  
Agarose Gel Electrophoresis ◽  
Circular Dna ◽  
T4 Dna Ligase

Abstract We have developed a simple procedure for determining if a plasmid DNA contains single strand interruptions that can be sealed by DNA ligase and thus contain single strand interruptions with 5'-phosphate and 3'-hydroxyl groups. In this procedure, plasmid DNAs with ligatable nicks were ligated by T4 DNA ligase, supercoiled by E. coli DNA gyrase and analyzed by agarose gel electrophoresis. Our results show that after sealing nicked circular DNA with DNA ligase, supercoiling by DNA gyrase produces a rapidly migrating DNA species that can easily be distinguished from nicked circular DNA by agarose gel electrophoresis.

Luminography – an Alternative Assay for Detection of von Willebrand Factor Multimers

1988 ◽  
Vol 60 (02) ◽  
pp. 133-136 ◽  
Author(s):  
R Schneppenheim ◽  
H Plendl ◽  
U Budde
Keyword(s):  
Von Willebrand Factor ◽  
Gel Electrophoresis ◽  
Detection Methods ◽  
Agarose Gel ◽  
Agarose Gel Electrophoresis ◽  
Luminescence Assay ◽  
Von Willebrand ◽  
Willebrand Factor

SummaryA luminescence assay was adapted for detection of von Willebrand factor multimers subsequent to SDS-agarose gel electrophoresis and electroblotting onto nitrocellulose. The method is as fast as chromogenic detection methods and appears to be as sensitive as autoradiography without the disadvantages of the latter.

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