Improved detection of lymphocyte membrane proteins in purified form and as a crude mixture using native and denaturing polyacrylamide gel electrophoresis by optimisation of Coomassie Brilliant Blue and silver staining

1990 ◽  
Vol 11 (1) ◽  
pp. 53-60 ◽  
Author(s):  
Robert S. Warlow ◽  
Claude C. A. Bernard
Keyword(s):  
Membrane Proteins ◽  
Silver Staining ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Polyacrylamide Gel ◽  
Brilliant Blue ◽  
Coomassie Brilliant Blue ◽  
Lymphocyte Membrane ◽  
Crude Mixture ◽  
Coomassie Brilliant

MEASUREMENT OF CANINE PITUITARY PROLACTIN

1973 ◽  
Vol 56 (2) ◽  
pp. 245-NP ◽  
Author(s):  
P. G. SALUJA ◽  
M. GRONOW ◽  
J. M. HAMILTON
Keyword(s):  
Isoelectric Focusing ◽  
Isoelectric Point ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel ◽  
Brilliant Blue ◽  
Coomassie Brilliant Blue ◽  
Ovine Prolactin ◽  
Pituitary Prolactin ◽  
Coomassie Brilliant

SUMMARY Isoelectric focusing in polyacrylamide gel followed by staining with Coomassie brilliant blue was used for the densitometric estimation of ovine prolactin standard and canine pituitary prolactin. The results were compared with those obtained by conventional polyacrylamide gel electrophoretic procedures and isoelectric focusing was found to be a valid technique for the estimation of prolactin and to offer greater technical convenience. The mobility of ovine and canine prolactin was similar in isoelectric focusing and gel electrophoresis. The isoelectric point of ovine and canine prolactin was found to be 6·17 and 6·61 respectively. Constant levels of prolactin were found in the pituitaries of bitches at oestrus.

Silver staining for proteins in SDS-polyacrylamide gel electrophoresis.

1986 ◽  
Vol 30 (3) ◽  
pp. 229-238 ◽  
Author(s):  
Tsutomu Inoue ◽  
Hiroaki Asaga ◽  
Mayumi Tamura
Keyword(s):  
Silver Staining ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Polyacrylamide Gel
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