MEASUREMENT OF CANINE PITUITARY PROLACTIN

SUMMARY Isoelectric focusing in polyacrylamide gel followed by staining with Coomassie brilliant blue was used for the densitometric estimation of ovine prolactin standard and canine pituitary prolactin. The results were compared with those obtained by conventional polyacrylamide gel electrophoretic procedures and isoelectric focusing was found to be a valid technique for the estimation of prolactin and to offer greater technical convenience. The mobility of ovine and canine prolactin was similar in isoelectric focusing and gel electrophoresis. The isoelectric point of ovine and canine prolactin was found to be 6·17 and 6·61 respectively. Constant levels of prolactin were found in the pituitaries of bitches at oestrus.

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Quantitation of proteins by elution of Coomassie brilliant blue R from stained bands after sodium dodecyl sulfate-polyacrylamide gel electrophoresis

Analytical Biochemistry ◽

10.1016/0003-2697(86)90218-6 ◽

1986 ◽

Vol 155 (1) ◽

pp. 23-27 ◽

Cited By ~ 90

Author(s):

Eric H. Ball

Keyword(s):

Sodium Dodecyl Sulfate ◽

Gel Electrophoresis ◽

Polyacrylamide Gel Electrophoresis ◽

Sodium Dodecyl ◽

Polyacrylamide Gel ◽

Brilliant Blue ◽

Coomassie Brilliant Blue ◽

Dodecyl Sulfate ◽

Coomassie Brilliant

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A modified coomassie brilliant blue G 250 staining method for the detection of chitinase activity and molecular weight after polyacrylamide gel electrophoresis

Journal of Bioscience and Bioengineering ◽

10.1263/jbb.106.111 ◽

2008 ◽

Vol 106 (1) ◽

pp. 111-113 ◽

Cited By ~ 21

Author(s):

Chun Yi Liau ◽

Chung-Sheng Lin

Keyword(s):

Molecular Weight ◽

Gel Electrophoresis ◽

Staining Method ◽

Polyacrylamide Gel Electrophoresis ◽

Chitinase Activity ◽

Polyacrylamide Gel ◽

Brilliant Blue ◽

Brilliant Blue G ◽

Coomassie Brilliant Blue ◽

Coomassie Brilliant

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Production and separation of peptides from proteins stained with Coomassie brilliant blue R-250 after separation by sodium dodecyl sulfate-polyacrylamide gel electrophoresis

Analytical Biochemistry ◽

10.1016/0003-2697(90)90227-z ◽

1990 ◽

Vol 191 (2) ◽

pp. 332-336 ◽

Cited By ~ 78

Author(s):

Hiroshi Kawasaki ◽

Yasufumi Emori ◽

Koichi Suzuki

Keyword(s):

Sodium Dodecyl Sulfate ◽

Gel Electrophoresis ◽

Polyacrylamide Gel Electrophoresis ◽

Sodium Dodecyl ◽

Polyacrylamide Gel ◽

Brilliant Blue ◽

Coomassie Brilliant Blue ◽

Dodecyl Sulfate ◽

Coomassie Brilliant

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Highly Sensitive and Fast Protein Detection with Coomassie Brilliant Blue in Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis

Bulletin of the Korean Chemical Society ◽

10.5012/bkcs.2002.23.11.1511 ◽

2002 ◽

Vol 23 (11) ◽

pp. 1511-1512 ◽

Cited By ~ 166

Keyword(s):

Sodium Dodecyl Sulfate ◽

Gel Electrophoresis ◽

Polyacrylamide Gel Electrophoresis ◽

Sodium Dodecyl ◽

Protein Detection ◽

Polyacrylamide Gel ◽

Brilliant Blue ◽

Coomassie Brilliant Blue ◽

Highly Sensitive ◽

Coomassie Brilliant

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Simultaneous staining of proteins during polyacrylamide gel electrophoresis in acidic gels by countermigration of Coomassie brilliant blue R-250

Analytical Biochemistry ◽

10.1016/0003-2697(83)90037-4 ◽

1983 ◽

Vol 132 (2) ◽

pp. 481-483 ◽

Cited By ~ 46

Author(s):

George Varghese ◽

A.M. Diwan

Keyword(s):

Gel Electrophoresis ◽

Polyacrylamide Gel Electrophoresis ◽

Polyacrylamide Gel ◽

Brilliant Blue ◽

Coomassie Brilliant Blue ◽

Coomassie Brilliant

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Colorimetric Measurement of Protein Stained with Coomassie Brilliant Blue R on Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis by Eluting with Formamide

Agricultural and Biological Chemistry ◽

10.1080/00021369.1986.10867672 ◽

1986 ◽

Vol 50 (7) ◽

pp. 1911-1912

Author(s):

Amane Makino ◽

Tadahiko Mae ◽

Koji Ohira

Keyword(s):

Sodium Dodecyl Sulfate ◽

Gel Electrophoresis ◽

Polyacrylamide Gel Electrophoresis ◽

Sodium Dodecyl ◽

Polyacrylamide Gel ◽

Brilliant Blue ◽

Coomassie Brilliant Blue ◽

Colorimetric Measurement ◽

Dodecyl Sulfate ◽

Coomassie Brilliant

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Colorimetric measurement of protein stained with coomassie brilliant blue R on sodium dodecyl sulfate-polyacrylamide gel electrophoresis by eluting with formamide.

Agricultural and Biological Chemistry ◽

10.1271/bbb1961.50.1911 ◽

1986 ◽

Vol 50 (7) ◽

pp. 1911-1912 ◽

Cited By ~ 53

Author(s):

Amane MAKINO ◽

Tadahiko MAE ◽

Koji OHIRA

Keyword(s):

Sodium Dodecyl Sulfate ◽

Gel Electrophoresis ◽

Polyacrylamide Gel Electrophoresis ◽

Sodium Dodecyl ◽

Polyacrylamide Gel ◽

Brilliant Blue ◽

Coomassie Brilliant Blue ◽

Colorimetric Measurement ◽

Dodecyl Sulfate ◽

Coomassie Brilliant

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Improved detection of lymphocyte membrane proteins in purified form and as a crude mixture using native and denaturing polyacrylamide gel electrophoresis by optimisation of Coomassie Brilliant Blue and silver staining

Electrophoresis ◽

10.1002/elps.1150110112 ◽

1990 ◽

Vol 11 (1) ◽

pp. 53-60 ◽

Cited By ~ 2

Author(s):

Robert S. Warlow ◽

Claude C. A. Bernard

Keyword(s):

Membrane Proteins ◽

Silver Staining ◽

Gel Electrophoresis ◽

Polyacrylamide Gel Electrophoresis ◽

Polyacrylamide Gel ◽

Brilliant Blue ◽

Coomassie Brilliant Blue ◽

Lymphocyte Membrane ◽

Crude Mixture ◽

Coomassie Brilliant

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Elution of proteins from polyacrylamide eels: a simple and economic procedure

Revista do Instituto de Medicina Tropical de São Paulo ◽

10.1590/s0036-46651989000100009 ◽

1989 ◽

Vol 31 (1) ◽

pp. 44-47

Author(s):

Vera Bongertz

Keyword(s):

Gel Electrophoresis ◽

Brilliant Blue ◽

Polyacrylamide Gels ◽

Coomassie Brilliant Blue ◽

Coomassie Brilliant

A simplified methodology for the quantitative electroelution of proteins from polyacrylamide gels is described. After staining with Coomassie Brilliant Blue R 250, the identified bands are excised from the gel and the proteins eluted using a procedure developed for use in conventional tube gel electrophoresis equipment.

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