Significant overexpression of the Merkel cell polyomavirus (MCPyV) large T antigen in Merkel cell carcinoma

Head & Neck ◽  
2012 ◽  
Vol 35 (2) ◽  
pp. 184-189 ◽  
Author(s):  
Boban M. Erovic ◽  
Ayman Al Habeeb ◽  
Luke Harris ◽  
David P. Goldstein ◽  
Danny Ghazarian ◽  
...  
Keyword(s):  
Cell Carcinoma ◽  
Merkel Cell Carcinoma ◽  
T Antigen ◽  
Merkel Cell Polyomavirus ◽  
Merkel Cell ◽  
Large T Antigen

scholarly journals Merkel Cell Polyomavirus Infection, Large T Antigen, Retinoblastoma Protein and Outcome in Merkel Cell Carcinoma

2011 ◽  
Vol 17 (14) ◽  
pp. 4806-4813 ◽  
Author(s):  
Harri Sihto ◽  
Heli Kukko ◽  
Virve Koljonen ◽  
Risto Sankila ◽  
Tom Böhling ◽  
...  
Keyword(s):  
Cell Carcinoma ◽  
Merkel Cell Carcinoma ◽  
Retinoblastoma Protein ◽  
T Antigen ◽  
Merkel Cell Polyomavirus ◽  
Merkel Cell ◽  
Large T Antigen

scholarly journals An intact retinoblastoma protein-binding site in Merkel cell polyomavirus large T antigen is required for promoting growth of Merkel cell carcinoma cells

2011 ◽  
Vol 130 (4) ◽  
pp. 847-856 ◽  
Author(s):  
Roland Houben ◽  
Christian Adam ◽  
Anne Baeurle ◽  
Sonja Hesbacher ◽  
Johannes Grimm ◽  
...  
Keyword(s):  
Protein Binding ◽  
Cell Carcinoma ◽  
Binding Site ◽  
Merkel Cell Carcinoma ◽  
Retinoblastoma Protein ◽  
T Antigen ◽  
Merkel Cell Polyomavirus ◽  
Carcinoma Cells ◽  
Merkel Cell ◽  
Large T Antigen

scholarly journals Serine 220 phosphorylation of the Merkel cell polyomavirus large T antigen crucially supports growth of Merkel cell carcinoma cells

2015 ◽  
Vol 138 (5) ◽  
pp. 1153-1162 ◽  
Author(s):  
David Schrama ◽  
Sonja Hesbacher ◽  
Sabrina Angermeyer ◽  
Andreas Schlosser ◽  
Sebastian Haferkamp ◽  
...  
Keyword(s):  
Cell Carcinoma ◽  
Merkel Cell Carcinoma ◽  
T Antigen ◽  
Merkel Cell Polyomavirus ◽  
Carcinoma Cells ◽  
Merkel Cell ◽  
Large T Antigen

scholarly journals RB1 is the crucial target of the Merkel cell polyomavirus Large T antigen in Merkel cell carcinoma cells

Oncotarget ◽  
2016 ◽  
Vol 7 (22) ◽  
pp. 32956-32968 ◽  
Author(s):  
Sonja Hesbacher ◽  
Lisa Pfitzer ◽  
Katharina Wiedorfer ◽  
Sabrina Angermeyer ◽  
Andreas Borst ◽  
...  
Keyword(s):  
Cell Carcinoma ◽  
Merkel Cell Carcinoma ◽  
T Antigen ◽  
Merkel Cell Polyomavirus ◽  
Carcinoma Cells ◽  
Merkel Cell ◽  
Large T Antigen

Merkel cell carcinoma: histopathologic and prognostic features according to the immunohistochemical expression of Merkel cell polyomavirus large T antigen correlated with viral load

2015 ◽  
Vol 46 (3) ◽  
pp. 443-453 ◽  
Author(s):  
Valérie Leroux-Kozal ◽  
Nicolas Lévêque ◽  
Véronique Brodard ◽  
Candice Lesage ◽  
Oriane Dudez ◽  
...  
Keyword(s):  
Viral Load ◽  
Cell Carcinoma ◽  
Merkel Cell Carcinoma ◽  
T Antigen ◽  
Merkel Cell Polyomavirus ◽  
Merkel Cell ◽  
Large T Antigen ◽  
Immunohistochemical Expression ◽  
Prognostic Features

scholarly journals Nuclear localization of Merkel cell polyomavirus large T antigen in Merkel cell carcinoma

Virology ◽  
2010 ◽  
Vol 398 (2) ◽  
pp. 273-279 ◽  
Author(s):  
Tomoyuki Nakamura ◽  
Yuko Sato ◽  
Daisuke Watanabe ◽  
Hideki Ito ◽  
Nozomi Shimonohara ◽  
...  
Keyword(s):  
Cell Carcinoma ◽  
Merkel Cell Carcinoma ◽  
Nuclear Localization ◽  
T Antigen ◽  
Merkel Cell Polyomavirus ◽  
Merkel Cell ◽  
Large T Antigen

scholarly journals Merkel Cell Polyomavirus Large T Antigen is Dispensable in G2 and M-Phase to Promote Proliferation of Merkel Cell Carcinoma Cells

Viruses ◽  
2020 ◽  
Vol 12 (10) ◽  
pp. 1162
Author(s):  
Roland Houben ◽  
Marlies Ebert ◽  
Sonja Hesbacher ◽  
Thibault Kervarrec ◽  
David Schrama
Keyword(s):  
Cell Cycle ◽  
Cell Carcinoma ◽  
Merkel Cell Carcinoma ◽  
T Antigen ◽  
Merkel Cell Polyomavirus ◽  
Carcinoma Cells ◽  
Merkel Cell ◽  
Large T Antigen ◽  
M Phase ◽  
G2 Phase

Merkel cell carcinoma (MCC) is an aggressive skin cancer frequently caused by the Merkel cell polyomavirus (MCPyV), and proliferation of MCPyV-positive MCC tumor cells depends on the expression of a virus-encoded truncated Large T antigen (LT) oncoprotein. Here, we asked in which phases of the cell cycle LT activity is required for MCC cell proliferation. Hence, we generated fusion-proteins of MCPyV-LT and parts of geminin (GMMN) or chromatin licensing and DNA replication factor1 (CDT1). This allowed us to ectopically express an LT, which is degraded either in the G1 or G2 phase of the cell cycle, respectively, in MCC cells with inducible T antigen knockdown. We demonstrate that LT expressed only in G1 is capable of rescuing LT knockdown-induced growth suppression while LT expressed in S and G2/M phases fails to support proliferation of MCC cells. These results suggest that the crucial function of LT, which has been demonstrated to be inactivation of the cellular Retinoblastoma protein 1 (RB1) is only required to initiate S phase entry.

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