scholarly journals Characterization of a sodium-dependent transport system for butyrobetaine into rat liver plasma membrane vesicles

Hepatology ◽  
1998 ◽  
Vol 28 (2) ◽  
pp. 521-525 ◽  
Author(s):  
Simona Berardi ◽  
Bruno Stieger ◽  
Sandra Wächter ◽  
Brigitte O'Neill ◽  
Stephan Krähenbühl
Keyword(s):  
Plasma Membrane ◽  
Rat Liver ◽  
Transport System ◽  
Membrane Vesicles ◽  
Plasma Membrane Vesicles ◽  
Liver Plasma Membrane ◽  
Sodium Dependent ◽  
Dependent Transport

Thiamine transport by basolateral rat liver plasma membrane vesicles

1992 ◽  
Vol 103 (3) ◽  
pp. 1056-1065 ◽  
Author(s):  
Richard H. Moseley ◽  
Pankaj G. Vashi ◽  
Suzanne M. Jarose ◽  
Chris J. Dickinson ◽  
Patricia A. Permoad
Keyword(s):  
Plasma Membrane ◽  
Rat Liver ◽  
Membrane Vesicles ◽  
Plasma Membrane Vesicles ◽  
Liver Plasma Membrane ◽  
Thiamine Transport

scholarly journals Characterization of Ca2+ fluxes in rat liver plasma-membrane vesicles

1988 ◽  
Vol 256 (1) ◽  
pp. 117-124 ◽  
Author(s):  
C Dargemont ◽  
M Hilly ◽  
M Claret ◽  
J P Mauger
Keyword(s):  
Plasma Membrane ◽  
Rat Liver ◽  
Uptake Rate ◽  
Ionic Composition ◽  
Membrane Vesicles ◽  
Plasma Membrane Vesicles ◽  
Microsomal Preparation ◽  
Intact Cells ◽  
Inside Out

Inside-out plasma-membrane vesicles isolated from rat liver [Prpic, Green, Blackmore & Exton (1984) J. Biol. Chem. 259, 1382-1385] accumulated a substantial amount of 45Ca2+ when they were incubated in a medium whose ionic composition and pH mimicked those of cytosol and which contained MgATP. The Vmax of the initial 45Ca2+ uptake rate was 2.9 +/- 0.6 nmol/min per mg and the Km for Ca2+ was 0.50 +/- 0.08 microM. The ATP-dependent 45Ca2+ uptake by inside-out plasma-membrane vesicles was about 20 times more sensitive to saponin than was the ATP-dependent uptake by a microsomal preparation. The 45Ca2+ efflux from the inside-out vesicles, which is equivalent to the Ca2+ influx in intact cells, was increased when the free Ca2+ concentration in the medium was decreased. The Ca2+ antagonists La3+ and Co2+ inhibited the 45Ca2+ efflux from the vesicles. Neomycin stimulated the Ca2+ efflux in the presence of either a high or a low free Ca2+ concentration. These results confirm that polyvalent cations regulate Ca2+ fluxes through the plasma membrane.

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