scholarly journals Wound-induced expression of a potato proteinase inhibitor II gene in transgenic tobacco plants

1987 ◽  
Vol 6 (2) ◽  
pp. 303-306 ◽  
Author(s):  
Jose J. Sanchez-Serrano ◽  
Michael Keil ◽  
Aileen O'Connor ◽  
Jeff Schell ◽  
Lothar Willmitzer
2004 ◽  
Vol 36 (8) ◽  
pp. 553-558 ◽  
Author(s):  
Yun-Jun Liu ◽  
Yuan Yuan ◽  
Jun Zheng ◽  
Ya-Zhong Tao ◽  
Zhi-Gang Dong ◽  
...  

Abstract The modified Cry1Ac was expressed in transgenic tobacco plants. To allow secretion of the Cry1Ac protein into the intercellular space, the signal peptide sequence of potato proteinase inhibitor II (pinII) was N-terminally fused to the Cry1Ac encoding region. Expression of Cry1Ac in transgenic tobacco plants was assayed with ELISA. The results showed that pinII signal peptide sequence enhanced the expression of Cry1Ac protein and led to the secretion of the Cry1Ac protein in transgenic tobacco plants. GFP gene was also fused to the signal peptide sequence and transformed to tobacco. The results of fluorescent detection showed that GFP had localized in the apoplast of transgenic plants.


1993 ◽  
Vol 21 (4) ◽  
pp. 655-663 ◽  
Author(s):  
Sameer A. Masoud ◽  
Lowell B. Johnson ◽  
Frank F. White ◽  
Gerald R. Reeck

PLoS ONE ◽  
2013 ◽  
Vol 8 (8) ◽  
pp. e71714 ◽  
Author(s):  
Fengdan Guo ◽  
Chuanliang Liu ◽  
Han Xia ◽  
Yuping Bi ◽  
Chuanzhi Zhao ◽  
...  

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