scholarly journals Spliced leader RNA of trypanosomes: in vivo mutational analysis reveals extensive and distinct requirements for trans splicing and cap4 formation.

1996 ◽  
Vol 15 (16) ◽  
pp. 4380-4391 ◽  
Author(s):  
S. Lücke ◽  
G. L. Xu ◽  
Z. Palfi ◽  
M. Cross ◽  
V. Bellofatto ◽  
...  
Keyword(s):  
Mutational Analysis ◽  
Spliced Leader ◽  
Trans Splicing ◽  
Spliced Leader Rna

scholarly journals An in vivo genetic screen for genes involved in spliced leader trans-splicing indicates a crucial role for continuous de novo spliced leader RNP assembly

2017 ◽  
Vol 45 (14) ◽  
pp. 8474-8483 ◽  
Author(s):  
Lucas Philippe ◽  
George C. Pandarakalam ◽  
Rotimi Fasimoye ◽  
Neale Harrison ◽  
Bernadette Connolly ◽  
...  
Keyword(s):  
Crucial Role ◽  
De Novo ◽  
Genetic Screen ◽  
Spliced Leader ◽  
Trans Splicing

scholarly journals The nematode spliced leader RNA participates in trans-splicing as an Sm snRNP.

1990 ◽  
Vol 9 (11) ◽  
pp. 3667-3673 ◽  
Author(s):  
P. A. Maroney ◽  
G. J. Hannon ◽  
J. A. Denker ◽  
T. W. Nilsen
Keyword(s):  
Spliced Leader ◽  
Trans Splicing ◽  
In Trans ◽  
Spliced Leader Rna

scholarly journals Temporal order of RNA-processing reactions in trypanosomes: rapid trans splicing precedes polyadenylation of newly synthesized tubulin transcripts.

1993 ◽  
Vol 13 (1) ◽  
pp. 720-725 ◽  
Author(s):  
E Ullu ◽  
K R Matthews ◽  
C Tschudi
Keyword(s):  
Rna Processing ◽  
Temporal Order ◽  
Kinetic Studies ◽  
Splice Acceptor ◽  
Mrna Synthesis ◽  
Alpha Tubulin ◽  
Spliced Leader ◽  
Polycistronic Transcription ◽  
Trans Splicing

Many trypanosome genes are expressed as part of large polycistronic transcription units. This finding suggests that regulation of mRNA biogenesis may emphasize RNA-processing reactions more so than in other organisms. This study was undertaken to understand the temporal order of two RNA-processing reactions, trans splicing and polyadenylation, in the maturation of trypanosome mRNAs in vivo. Kinetic studies revealed rapid trans splicing of alpha-tubulin, beta-tubulin, and actin pre-mRNAs within 1 to 2 min after synthesis of the 3' splice site. Furthermore, following blockage of pre-mRNA synthesis, newly synthesized spliced leader RNA cannot be used for trans splicing, suggesting that trypanosomes do not accumulate substantial amounts of pre-mRNA which can provide splice acceptor sites. Thus, trans splicing is cotranscriptional. In addition, we show that trans splicing precedes polyadenylation in the processing of trypanosome tubulin pre-mRNAs.

scholarly journals Spliced leader RNA trans-splicing in dinoflagellates

2007 ◽  
Vol 104 (11) ◽  
pp. 4618-4623 ◽  
Author(s):  
H. Zhang ◽  
Y. Hou ◽  
L. Miranda ◽  
D. A. Campbell ◽  
N. R. Sturm ◽  
...  
Keyword(s):  
Spliced Leader ◽  
Trans Splicing ◽  
Spliced Leader Rna

trans-spliced Caenorhabditis elegans mRNAs retain trimethylguanosine caps

1990 ◽  
Vol 10 (4) ◽  
pp. 1764-1768
Author(s):  
R F Liou ◽  
T Blumenthal
Keyword(s):  
Caenorhabditis Elegans ◽  
Antibody Binding ◽  
Small Nuclear Rna ◽  
Nematode Caenorhabditis Elegans ◽  
Spliced Leader ◽  
C Elegans ◽  
Distinct Subset ◽  
Trans Splicing ◽  
Nuclear Rna ◽  
Spliced Leader Rna

The nematode Caenorhabditis elegans has an unusual small nuclear RNA, containing a 100-nucleotide RNA molecule, spliced leader RNA, which donates its 5' 22 nucleotides to a variety of recipient RNAs by a trans-splicing reaction. The spliced leader RNA has a 5' trimethylguanosine (TMG) cap, which becomes the 5' end of trans-spliced mRNAs. We found that mature trans-spliced mRNAs were immunoprecipitable with anti-TMG cap antibodies and that TMG-containing dinucleotides specifically competed with the trans-spliced mRNAs for antibody binding. We also found that these mRNAs retained their TMG caps throughout development and that the TMG-capped mRNAs were polysome associated. Since the large majority of C. elegans mRNAs are not trans-spliced, the addition of the spliced leader and its TMG cap to a limited group of recipient RNAs may create a functionally distinct subset of mRNAs.

scholarly journals Spliced-Leader RNA trans Splicing in a Chordate, Oikopleura dioica, with a Compact Genome

2004 ◽  
Vol 24 (17) ◽  
pp. 7795-7805 ◽  
Author(s):  
Philippe Ganot ◽  
Torben Kallesøe ◽  
Richard Reinhardt ◽  
Daniel Chourrout ◽  
Eric M. Thompson
Keyword(s):  
Caenorhabditis Elegans ◽  
Regulatory Elements ◽  
Sm Proteins ◽  
Oikopleura Dioica ◽  
Spliced Leader ◽  
Trans Splicing ◽  
Spliced Leader Rna

ABSTRACT trans splicing of a spliced-leader RNA (SL RNA) to the 5′ ends of mRNAs has been shown to have a limited and sporadic distribution among eukaryotes. Within metazoans, only nematodes are known to process polycistronic pre-mRNAs, produced from operon units of transcription, into mature monocistronic mRNAs via an SL RNA trans-splicing mechanism. Here we demonstrate that a chordate with a highly compact genome, Oikopleura dioica, now joins Caenorhabditis elegans in coupling trans splicing with processing of polycistronic transcipts. We identified a single SL RNA which associates with Sm proteins and has a trimethyl guanosine cap structure reminiscent of spliceosomal snRNPs. The same SL RNA, estimated to be trans-spliced to at least 25% of O. dioica mRNAs, is used for the processing of both isolated or first cistrons and downstream cistrons in a polycistronic precursor. Remarkably, intercistronic regions in O. dioica are far more reduced than those in either nematodes or kinetoplastids, implying minimal cis-regulatory elements for coupling of 3′-end formation and trans splicing.

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