scholarly journals Flexible polygon-mirror based laser scanning microscope platform for multiphoton in-vivo imaging

2017 ◽  
Vol 10 (11) ◽  
pp. 1526-1537 ◽  
Author(s):  
Y. X. Li ◽  
V. Gautam ◽  
A. Brüstle ◽  
I. A. Cockburn ◽  
V. R. Daria ◽  
...  



2006 ◽  
Vol 400 (1-2) ◽  
pp. 53-57 ◽  
Author(s):  
Yoshiyuki Fukuda ◽  
Yoshihiro Kawano ◽  
Yoshihisa Tanikawa ◽  
Masahiro Oba ◽  
Masafumi Koyama ◽  
...  






Cornea ◽  
2006 ◽  
Vol 25 (2) ◽  
pp. 127-131 ◽  
Author(s):  
Alexander Eckard ◽  
Joachim Stave ◽  
Rudolf F Guthoff


2011 ◽  
Vol 19 (5) ◽  
pp. 4357 ◽  
Author(s):  
Sung Min Choi ◽  
Wi Han Kim ◽  
Daniel Côté ◽  
Cheol-Woo Park ◽  
Ho Lee


2003 ◽  
Author(s):  
Juergen Lademann ◽  
H. Richter ◽  
N. Otberg ◽  
F. Lawrenz ◽  
U. Blume-Peytavi ◽  
...  


Author(s):  
Thomas M. Jovin ◽  
Michel Robert-Nicoud ◽  
Donna J. Arndt-Jovin ◽  
Thorsten Schormann

Light microscopic techniques for visualizing biomolecules and biochemical processes in situ have become indispensable in studies concerning the structural organization of supramolecular assemblies in cells and of processes during the cell cycle, transformation, differentiation, and development. Confocal laser scanning microscopy offers a number of advantages for the in situ localization and quantitation of fluorescence labeled targets and probes: (i) rejection of interfering signals emanating from out-of-focus and adjacent structures, allowing the “optical sectioning” of the specimen and 3-D reconstruction without time consuming deconvolution; (ii) increased spatial resolution; (iii) electronic control of contrast and magnification; (iv) simultanous imaging of the specimen by optical phenomena based on incident, scattered, emitted, and transmitted light; and (v) simultanous use of different fluorescent probes and types of detectors.We currently use a confocal laser scanning microscope CLSM (Zeiss, Oberkochen) equipped with 3-laser excitation (u.v - visible) and confocal optics in the fluorescence mode, as well as a computer-controlled X-Y-Z scanning stage with 0.1 μ resolution.



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