Malignant transformationin Vitro of chinese hamster embryonic fibroblasts with the Schmidt-Ruppin strain of Rous sarcoma virus and karyological analysis of this process

1966 ◽  
Vol 68 (3) ◽  
pp. 221-235 ◽  
Author(s):  
Ivo Hlo?�nek ◽  
Ludv�k Donner ◽  
Jan Svoboda
Keyword(s):  
Rous Sarcoma Virus ◽  
Chinese Hamster ◽  
Embryonic Fibroblasts ◽  
Karyological Analysis ◽  
Rous Sarcoma ◽  
Sarcoma Virus

Karyological analysis of Rous sarcoma virus-producing rat-kangaroo cell lines

1973 ◽  
Vol 12 (2) ◽  
pp. 117-127 ◽  
Author(s):  
L. Donner ◽  
H. Sainerová ◽  
J. Svoboda
Keyword(s):  
Cell Lines ◽  
Rous Sarcoma Virus ◽  
Karyological Analysis ◽  
Rous Sarcoma ◽  
Sarcoma Virus

scholarly journals Rous sarcoma virus mutant dlPA105 induces different transformed phenotypes in quail embryonic fibroblasts and neuroretina cells.

1987 ◽  
Vol 61 (8) ◽  
pp. 2530-2539 ◽  
Author(s):  
F Poirier ◽  
D Laugier ◽  
M Marx ◽  
G Dambrine ◽  
E A Garber ◽  
...  
Keyword(s):  
Rous Sarcoma Virus ◽  
Embryonic Fibroblasts ◽  
Rous Sarcoma ◽  
Sarcoma Virus ◽  
Virus Mutant

scholarly journals Cholera toxin treatment stimulates tumorigenicity of Rous sarcoma virus-transformed cells.

1984 ◽  
Vol 4 (12) ◽  
pp. 2639-2642 ◽  
Author(s):  
M M Gottesman ◽  
C Roth ◽  
G Vlahakis ◽  
I Pastan
Keyword(s):  
Cyclic Amp ◽  
Cholera Toxin ◽  
Chinese Hamster Ovary ◽  
Rous Sarcoma Virus ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Tumor Formation ◽  
Ovary Cells ◽  
Rous Sarcoma ◽  
Sarcoma Virus

Chinese hamster ovary cells transformed by Rous sarcoma virus form tumors poorly in nude mice. Tumorigenicity was markedly stimulated by pretreatment of the cells with cholera toxin, which raises cyclic AMP levels and activates cyclic AMP-dependent protein kinase. Increased tumorigenicity was manifested by a severalfold increase in the rate of tumor formation, as well as earlier appearance and more rapid growth of tumors. In contrast, spontaneously transformed Chinese hamster ovary cells showed decreased tumorigenicity after cholera toxin treatment. The activation of tumorigenic potential in Rous sarcoma virus-transformed Chinese hamster ovary cells by cholera toxin correlated with increased phosphorylation of the viral oncogene product pp60src and stimulation of its tyrosine kinase activity.

Cholera toxin treatment stimulates tumorigenicity of Rous sarcoma virus-transformed cells

1984 ◽  
Vol 4 (12) ◽  
pp. 2639-2642
Author(s):  
M M Gottesman ◽  
C Roth ◽  
G Vlahakis ◽  
I Pastan
Keyword(s):  
Cyclic Amp ◽  
Cholera Toxin ◽  
Chinese Hamster Ovary ◽  
Rous Sarcoma Virus ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Tumor Formation ◽  
Ovary Cells ◽  
Rous Sarcoma ◽  
Sarcoma Virus

Chinese hamster ovary cells transformed by Rous sarcoma virus form tumors poorly in nude mice. Tumorigenicity was markedly stimulated by pretreatment of the cells with cholera toxin, which raises cyclic AMP levels and activates cyclic AMP-dependent protein kinase. Increased tumorigenicity was manifested by a severalfold increase in the rate of tumor formation, as well as earlier appearance and more rapid growth of tumors. In contrast, spontaneously transformed Chinese hamster ovary cells showed decreased tumorigenicity after cholera toxin treatment. The activation of tumorigenic potential in Rous sarcoma virus-transformed Chinese hamster ovary cells by cholera toxin correlated with increased phosphorylation of the viral oncogene product pp60src and stimulation of its tyrosine kinase activity.

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