Studies on the regulation of the three enzymes of the leloir pathway in cultured mammalian cells. I. Effect of substitution of galactose for glucose as the sole hexose in the medium in human diploid cell strains and in a rat hepatoma line

1975 ◽  
Vol 86 (1) ◽  
pp. 91-103 ◽  
Author(s):  
Elizabeth Sell Stern ◽  
Robert S. Krooth
Keyword(s):  
Mammalian Cells ◽  
Diploid Cell ◽  
Human Diploid Cell ◽  
Leloir Pathway ◽  
Cell Strains ◽  
Rat Hepatoma

Fate of HL-A antigens in aging cultured human diploid cell strains

1973 ◽  
Vol 78 (2) ◽  
pp. 367-375 ◽  
Author(s):  
C. Brautbar ◽  
M.A. Pellegrino ◽  
S. Ferrone ◽  
R.A. Reisfeld ◽  
R. Payne ◽  
...  
Keyword(s):  
Diploid Cell ◽  
Human Diploid Cell ◽  
Cell Strains

Homologous plasmid recombination is elevated in immortally transformed cells

1989 ◽  
Vol 9 (9) ◽  
pp. 4009-4017
Author(s):  
G K Finn ◽  
B W Kurz ◽  
R Z Cheng ◽  
R J Shmookler Reis
Keyword(s):  
Mammalian Cells ◽  
Recombinant Dna ◽  
Diploid Cell ◽  
T Antigen ◽  
Transformed Cells ◽  
Standard Curve ◽  
Plasmid Recombination ◽  
Control Segment ◽  
Linear Molecules ◽  
Cell Strains

The levels of intramolecular plasmid recombination, following transfection of a plasmid substrate for homologous recombination into normal and immortally transformed cells, have been examined by two independent assays. In the first assay, recovered plasmid was tested for DNA rearrangements which regenerate a functional neomycin resistance gene from two overlapping fragments. Following transformation of bacteria, frequencies of recombinationlike events were determined from the ratio of neomycin-resistant (recombinant) colonies to ampicillin-resistant colonies (indicating total plasmid recovery). Such events, yielding predominantly deletions between the directly repeated sequences, were substantially more frequent in five immortal cell lines than in any of three normal diploid cell strains tested. Effects of plasmid replication or interaction with T antigen and of bacterially mediated rejoining of linear molecules generated in mammalian cells were excluded by appropriate controls. The second assay used limited coamplification of a control segment of plasmid DNA, and of the predicted recombinant DNA region, primed by two sets of flanking oligonucleotides. Each amplified band was quantitated by reference to a near-linear standard curve generated concurrently, and recombination frequencies were determined from the ratio of recombinant/control DNA regions. The results confirmed that recombinant DNA structures were generated within human cells at direct repeats in the transfected plasmid and were markedly more abundant in an immortal cell line than in the diploid normal cells from which that line was derived.

Isozyme polymorphisms in human diploid cell strains for research on cellular aging

1991 ◽  
Vol 26 (5) ◽  
pp. 441-451 ◽  
Author(s):  
Yoko Tasaki ◽  
Takane Ohara ◽  
Mari Yamamoto ◽  
Kiyotaka Yamamoto
Keyword(s):  
Diploid Cell ◽  
Cellular Aging ◽  
Human Diploid Cell ◽  
Cell Strains

Bridging the Gap: Human Diploid Cell Strains and the Origin of AIDS

2000 ◽  
Vol 204 (4) ◽  
pp. 497-503 ◽  
Author(s):  
BILLI GOLDBERG ◽  
RAPHAEL B STRICKER
Keyword(s):  
Diploid Cell ◽  
Human Diploid Cell ◽  
Cell Strains

Possibilities of vaccine manufacture in human diploid cell strains with a serum replacement factor

Biologicals ◽  
1991 ◽  
Vol 19 (3) ◽  
pp. 213-218 ◽  
Author(s):  
Francisco J. Candal ◽  
Velma G. George ◽  
Edwin W. Ades
Keyword(s):  
Diploid Cell ◽  
Human Diploid Cell ◽  
Serum Replacement ◽  
Cell Strains ◽  
Replacement Factor
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