The characterization of SV40-transformed cell lines derived from mouse teratocarcinoma: Growth properties and differentiated characteristics

Four transformed cell lines were established from cultures of human embryo kidney (HEK) cells microinjected or transfected with cloned adenovirus 12 (Ad12) EcoRI-C DNA (0 through 16.5 map units of the left-hand end of the viral genome). Each cell line showed a different growth pattern. Southern blotting demonstrated that all of the cell lines contained Ad12-specific DNA sequences, but in the microinjected isolates these were at a much lower copy number than in the transfected isolate. Two cell lines (Ad12 HEK 1 and 3) appeared to contain tandemly repeated Ad12 EcoRI-C DNA fragments. Immunoprecipitation and Western blotting confirmed that Ad12 early region 1 (E1) proteins were being expressed by all four of the transformed cell lines, but indicated that E1A polypeptide expression was considerably less than E1B polypeptide expression. All of the Ad12-transformed HEK cell lines were tumorigenic when inoculated intracranially into athymic nude mice.

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Isolation and partial characterization of virus-transformed cell lines representing the A, G and variant complementation groups of xeroderma pigmentosum

Mutation Research/DNA Repair Reports ◽

10.1016/0167-8817(86)90052-0 ◽

1986 ◽

Vol 165 (3) ◽

pp. 175-184 ◽

Cited By ~ 7

Author(s):

Dina P. Barbis ◽

Roger A. Schultz ◽

Errol C. Friedberg

Keyword(s):

Cell Lines ◽

Xeroderma Pigmentosum ◽

Partial Characterization ◽

Transformed Cell ◽

Complementation Groups ◽

Transformed Cell Lines

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Isolation and characterization of four adenovirus type 12-transformed human embryo kidney cell lines.

Molecular and Cellular Biology ◽

10.1128/mcb.4.1.110 ◽

1984 ◽

Vol 4 (1) ◽

pp. 110-116 ◽

Cited By ~ 35

Author(s):

J L Whittaker ◽

P J Byrd ◽

R J Grand ◽

P H Gallimore

Keyword(s):

Cell Lines ◽

Dna Sequences ◽

Human Embryo ◽

Adenovirus Type ◽

Left Hand ◽

Transformed Cell ◽

Isolation And Characterization ◽

Human Embryo Kidney ◽

Transformed Cell Lines

Four transformed cell lines were established from cultures of human embryo kidney (HEK) cells microinjected or transfected with cloned adenovirus 12 (Ad12) EcoRI-C DNA (0 through 16.5 map units of the left-hand end of the viral genome). Each cell line showed a different growth pattern. Southern blotting demonstrated that all of the cell lines contained Ad12-specific DNA sequences, but in the microinjected isolates these were at a much lower copy number than in the transfected isolate. Two cell lines (Ad12 HEK 1 and 3) appeared to contain tandemly repeated Ad12 EcoRI-C DNA fragments. Immunoprecipitation and Western blotting confirmed that Ad12 early region 1 (E1) proteins were being expressed by all four of the transformed cell lines, but indicated that E1A polypeptide expression was considerably less than E1B polypeptide expression. All of the Ad12-transformed HEK cell lines were tumorigenic when inoculated intracranially into athymic nude mice.

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