Isolation and characterization of revertants of the mammalian temperature sensitive leucyl-tRNA synthetase mutanttsHI

1979 ◽  
Vol 98 (2) ◽  
pp. 327-339 ◽  
Author(s):  
S. J. Molnar ◽  
L. H. Thompson ◽  
D. J. Lofgren ◽  
A. M. Rauth
Keyword(s):  
Trna Synthetase ◽  
Temperature Sensitive ◽  
Isolation And Characterization

Isolation and characterization of a variant myoblast cell line that is temperature sensitive for differentiation

1988 ◽  
Vol 8 (6) ◽  
pp. 2335-2341
Author(s):  
R J Akhurst ◽  
N B Flavin ◽  
J Worden
Keyword(s):  
Cell Line ◽  
Progenitor Cell ◽  
Temperature Shift ◽  
Temperature Sensitive ◽  
Isolation And Characterization ◽  
New Variant ◽  
Myoblast Cell Line ◽  
Myoblast Cell ◽  
Progenitor Cell Line

A new variant rat myogenic cell line, ts485, was isolated by subcloning the cell line ts3b2 (H. T. Nguyen, R. M. Medford, and B. Nadal-Ginard, Cell 34:281-293, 1983). Unlike the progenitor cell line, ts485 was thermosensitive for differentiation. Experiments with conditioned medium suggested that diffusible extracellular factors were not involved in dictating the differential phenotypes of ts485 cells cultured at the permissive and nonpermissive temperatures. Temperature shift experiments performed on cultures of ts485 cells indicated that the temperature-sensitive lesion was in a factor active during the growth phase and required to trigger a cascade of events leading to terminal differentiation.

Isolation and characterization of further defective clones of a temperature sensitive mutant (ts-1) of respiratory syncytial virus

1977 ◽  
Vol 54 (1-2) ◽  
pp. 53-60 ◽  
Author(s):  
Linda S. Richardson ◽  
T. J. Schnitzer ◽  
R. B. Belshe ◽  
Ena Camargo ◽  
D. A. Prevar ◽  
...  
Keyword(s):  
Respiratory Syncytial Virus ◽  
Temperature Sensitive ◽  
Sensitive Mutant ◽  
Temperature Sensitive Mutant ◽  
Isolation And Characterization ◽  
Syncytial Virus

Genetic and biochemical studies of asparagine-linked oligosaccharide assembly

1982 ◽  
Vol 300 (1099) ◽  
pp. 207-223 ◽  
Keyword(s):  
Protein Synthesis ◽  
Temperature Sensitive ◽  
Temperature Sensitive Mutants ◽  
Isolation And Characterization ◽  
Oligosaccharide Processing ◽  
Biochemical Studies ◽  
The Common ◽  
Specific Lipid ◽  
Temperature Sensitive Phenotype

The formation of N -glycosidic linkages of eukaryotic glycoproteins involves the assembly of a specific lipid-linked precursor oligosaccharide in the endoplasmic reticulum. This oligosaccharide is transferred from the lipid carrier to appropriate asparagine residues during protein synthesis. The protein-linked oligosaccharide then undergoes processing reactions that include both removal and addition of carbohydrate residues. In this paper we report recent studies from our laboratory on the synthesis of asparagine-linked oligosaccharides. In the first part we describe the isolation and characterization of temperature-sensitive mutants of yeast blocked at specific stages in the assembly of the lipid-linked oligosaccharide. In addition, we are using these mutants to clone the genes for the enzymes in this pathway by complementation of the temperature-sensitive phenotype. The second part deals with the topography of asparagine-linked oligosaccharide assembly. Our studies on the transmembrane movement of sugar residues during the assembly of secreted glycoproteins from cytoplasmic precursors are presented. Finally, experiments on the control of protein-linked oligosaccharide processing are described. Recent data are presented on the problem of how specific oligosaccharides are assembled from the common precursors at individual sites on glycoproteins.

scholarly journals Isolation and characterization of a variant myoblast cell line that is temperature sensitive for differentiation.

1988 ◽  
Vol 8 (6) ◽  
pp. 2335-2341 ◽  
Author(s):  
R J Akhurst ◽  
N B Flavin ◽  
J Worden
Keyword(s):  
Cell Line ◽  
Progenitor Cell ◽  
Temperature Shift ◽  
Temperature Sensitive ◽  
Isolation And Characterization ◽  
New Variant ◽  
Myoblast Cell Line ◽  
Myoblast Cell ◽  
Progenitor Cell Line

A new variant rat myogenic cell line, ts485, was isolated by subcloning the cell line ts3b2 (H. T. Nguyen, R. M. Medford, and B. Nadal-Ginard, Cell 34:281-293, 1983). Unlike the progenitor cell line, ts485 was thermosensitive for differentiation. Experiments with conditioned medium suggested that diffusible extracellular factors were not involved in dictating the differential phenotypes of ts485 cells cultured at the permissive and nonpermissive temperatures. Temperature shift experiments performed on cultures of ts485 cells indicated that the temperature-sensitive lesion was in a factor active during the growth phase and required to trigger a cascade of events leading to terminal differentiation.

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