The RyR–Cl Ca –VDCC axis contributes to spontaneous tone in urethral smooth muscle

2019 ◽  
Vol 234 (12) ◽  
pp. 23256-23267 ◽  
Author(s):  
Mei Feng ◽  
Zhaoyang Wang ◽  
Zheng Liu ◽  
Donghai Liu ◽  
Kaizhi Zheng ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Spontaneous Tone ◽  
Urethral Smooth Muscle

Non-Adrenergic Binding Sites for the “α-Antagonist” [3H] Idazoxan in Rabbit Urethral Smooth Muscle

1991 ◽  
Vol 11 (1-4) ◽  
pp. 153-162 ◽  
Author(s):  
F. Yablonsky ◽  
J. Y. Lacolle ◽  
J. P. Dausse
Keyword(s):  
Smooth Muscle ◽  
Binding Sites ◽  
Urethral Smooth Muscle

scholarly journals Vasomotor Responses of Rat Intracerebral Arterioles to Vasoactive Intestinal Peptide, Substance P, Neuropeptide Y, and Bradykinin

1988 ◽  
Vol 8 (2) ◽  
pp. 254-261 ◽  
Author(s):  
Ralph G. Dacey ◽  
John E. Bassett ◽  
Masakazu Takayasu
Keyword(s):  
Smooth Muscle ◽  
Substance P ◽  
Neuropeptide Y ◽  
Vasoactive Intestinal Peptide ◽  
Vessel Diameter ◽  
Cerebral Microcirculation ◽  
Vasoactive Peptides ◽  
Spontaneous Tone ◽  
Ph Dependent ◽  
Dose Dependent

The effect of vasoactive peptides on vascular smooth muscle in the cerebral microcirculation was examined using an isolated intracerebral arteriole preparation. Extraluminally applied vasoactive intestinal peptide (VIP) dilated the spontaneous tone of intracerebral arterioles to 118.9 ± 3.1% of control diameter at pH 7.30, with an EC50 of 7.27 × 10−8 M. Similar degrees of dilation to VIP were seen in vessels preconstricted by changing bath solution to pH 7.60. Substance P had no effect on vessel diameter at pH 7.30. However, in vessels precontracted by pH 7.60, significant dose-dependent dilation was observed with an EC50 of 2.55 × 10−10 M. Neuropeptide constricted intracerebral arterioles to 8l.22 ± 2.7% of control diameter, with an EC50 of 6.23 × 10−10 M. Bradykinin dilated intracerebral arterioles at pH 7.30 and pH 7.60 to 130 ± 3.0% of control diameter. VIP and bradykinin are potent vasodilators of intracerebral arterioles. Neuropeptide Y is a vasoconstrictor. The effect of substance P appeared to be either pH-dependent or dependent on some degree of precontraction by another agonist, but no effect on vessel diameter was seen at pH 7.30.

scholarly journals ROLES OF cAMP AND cGMP ON NON-ADRENERGIC, NON-CHOLINERGIC RELAXATION IN RABIT URETHRAL SMOOTH MUSCLE

1994 ◽  
Vol 85 (2) ◽  
pp. 314-320
Author(s):  
Takashi Morita ◽  
Shun Kondo ◽  
Masaki Yoshida
Keyword(s):  
Smooth Muscle ◽  
Urethral Smooth Muscle ◽  
Camp And Cgmp

Modulation of Spontaneous Electrical Activity in Urethral Smooth Muscle

2003 ◽  
Vol 35 (3/4) ◽  
pp. 217-223
Author(s):  
N. G. McHale ◽  
K. D. Thornbury ◽  
M. A. Hollywood ◽  
G. P. Sergeant
Keyword(s):  
Smooth Muscle ◽  
Electrical Activity ◽  
Spontaneous Electrical Activity ◽  
Urethral Smooth Muscle

C1 INVESTIGATING THE PHARMACOLOGY OF HUMAN URETHRAL SMOOTH MUSCLE: IN VITRO STUDY

2010 ◽  
Vol 9 (6) ◽  
pp. 619
Author(s):  
G.T. Kedia ◽  
S. Ückert ◽  
M. Kedia ◽  
L. Managadze ◽  
M.A. Kuczyk
Keyword(s):  
Smooth Muscle ◽  
In Vitro Study ◽  
Vitro Study ◽  
Urethral Smooth Muscle

scholarly journals Mediators and mechanisms of relaxation in rabbit urethral smooth muscle

1998 ◽  
Vol 123 (4) ◽  
pp. 617-624 ◽  
Author(s):  
Kristian Waldeck ◽  
Lars Ny ◽  
Katarina Persson ◽  
Karl-Erik Andersson
Keyword(s):  
Smooth Muscle ◽  
Urethral Smooth Muscle

Pharmacological Effects of Berberine and Palmatine on the Prostatic and Urethral Smooth Muscle of the Rabbit

2002 ◽  
Vol 6 (2) ◽  
pp. 62 ◽  
Author(s):  
Seung-June Oh ◽  
Jeongyoon Kang ◽  
Jeong Yun Jeong ◽  
Kyung Hoon Lee ◽  
Sung Joon Kim ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Pharmacological Effects ◽  
Urethral Smooth Muscle

Non-Cholinergic, Non-Adrenergic Nerve Mediated Relaxation of Trigone, Bladder Neck and Urethral Smooth Muscle in Vitro

1983 ◽  
Vol 129 (4) ◽  
pp. 848-850 ◽  
Author(s):  
Peter Klarskov ◽  
Thomas C. Gerstenberg ◽  
Daniel Ramirez ◽  
Tage Hald
Keyword(s):  
Smooth Muscle ◽  
Bladder Neck ◽  
Adrenergic Nerve ◽  
Urethral Smooth Muscle

scholarly journals Rho-associated kinase plays a role in rabbit urethral smooth muscle contraction, but not via enhanced myosin light chain phosphorylation

2011 ◽  
Vol 300 (1) ◽  
pp. F73-F85 ◽  
Author(s):  
Michael P. Walsh ◽  
Keith Thornbury ◽  
William C. Cole ◽  
Gerard Sergeant ◽  
Mark Hollywood ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Muscle Contraction ◽  
Light Chain ◽  
Actin Polymerization ◽  
Myosin Light Chain ◽  
Smooth Muscle Contraction ◽  
Regulatory Light Chains ◽  
Kinase C ◽  
Protein Kinase C Inhibitor ◽  
Urethral Smooth Muscle

The involvement of Rho-associated kinase (ROK) in activation of rabbit urethral smooth muscle contraction was investigated by examining the effects of two structurally distinct inhibitors of ROK, Y27632 and H1152, on the contractile response to electric field stimulation, membrane depolarization with KCl, and α1-adrenoceptor stimulation with phenylephrine. Both compounds inhibited contractions elicited by all three stimuli. The protein kinase C inhibitor GF109203X, on the other hand, had no effect. Urethral smooth muscle strips were analyzed for phosphorylation of three potential direct or indirect substrates of ROK: 1) myosin regulatory light chains (LC20) at S19, 2) the myosin-targeting subunit of myosin light chain phosphatase (MYPT1) at T697 and T855, and 3) cofilin at S3. The following results were obtained: 1) under resting tension, LC20 was phosphorylated to 0.65 ± 0.02 mol Pi/mol LC20 ( n = 21) at S19; 2) LC20 phosphorylation did not change in response to KCl or phenylephrine; 3) ROK inhibition had no effect on LC20 phosphorylation in the absence or presence of contractile stimuli; 4) under resting conditions, MYPT1 was partially phosphorylated at T697 and T855 and cofilin at S3; 5) phosphorylation of MYPT1 and cofilin was unaffected by KCl or phenylephrine; and 6) KCl- and phenylephrine-induced contraction-relaxation cycles did not correlate with actin polymerization-depolymerization. We conclude that ROK plays an important role in urethral smooth muscle contraction, but not via inhibition of MLCP or polymerization of actin.

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