Culture medium and protein supplementation influence in vitro fertilization and embryo development in the domestic cat

1991 ◽  
Vol 257 (3) ◽  
pp. 350-359 ◽  
Author(s):  
L. A. Johnston ◽  
A. M. Donoghue ◽  
S. J. O'brien ◽  
D. E. Wildt
Keyword(s):  
In Vitro Fertilization ◽  
Embryo Development ◽  
Culture Medium ◽  
Protein Supplementation ◽  
Domestic Cat ◽  
Vitro Fertilization

Exogenous glutathione supplementation in culture medium improves the bovine embryo development after in vitro fertilization

2015 ◽  
Vol 84 (5) ◽  
pp. 716-723 ◽  
Author(s):  
Wei-Jun Sun ◽  
Yun-Wei Pang ◽  
Yan Liu ◽  
Hai-Sheng Hao ◽  
Xue-Ming Zhao ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Embryo Development ◽  
Culture Medium ◽  
Bovine Embryo ◽  
Vitro Fertilization

Attempts at in vitro fertilization and culture of in vitro matured oocytes in sei (Balaenoptera borealis) and Bryde's (B. edeni) whales

Zygote ◽  
2009 ◽  
Vol 17 (1) ◽  
pp. 19-28 ◽  
Author(s):  
M. M. U. Bhuiyan ◽  
Y. Suzuki ◽  
H. Watanabe ◽  
K. Matsuoka ◽  
Y. Fujise ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Bovine Serum Albumin ◽  
Embryo Culture ◽  
Culture Medium ◽  
Cumulus Cells ◽  
Protein Supplementation ◽  
Vitro Fertilization ◽  
Embryo Culture Medium ◽  
Morula Stage

SummaryThe cumulus–oocyte–complexes (COCs) recovery rates with respect to reproductive status per sei (Balaenoptera borealis) and Bryde's (B. edeni) whales were determined in Experiment 1. The number of COCs recovered ranged from 16.0 to 30.6 and from 6.7 to 26.8 per sei and Bryde's whales, respectively. The effects of COCs grades and protein supplementation in embryo culture medium on development of in vitro fertilized (IVF) embryos were evaluated in sei and Bryde's whales in Experiment 2. The COCs were classified into either Grade A (COCs with five or more layers of compact cumulus cells) or Grade B (COCs with less than five layers of compact or expanded cumulus cells) before being cultured for IVM. The cleavage (12.0 to 19.5%), 4-cell (8.0 to 12.0%) and 8-cell (4.0 to 8.0%) formation rates in sei whales did not vary significantly between embryos derived from either grade A or B oocytes and between embryos cultured in either fetal whale serum (FWS)- or bovine serum albumin (BSA)-supplemented medium. The cleavage (4.0 to 14.8%), 4-cell (0.0 to 7.5%) and 8-cell (0.0 to 2.6%) formation rates in Bryde's whales did not vary significantly between embryos derived from either grade A or B oocytes and between embryos cultured in either FWS- or BSA-supplemented medium. The grade B oocytes cultured in FWS-supplemented medium developed to morula stage (1.1%) in sei whales. In conclusion, the present study indicates that IVF in sei whales is possible to achieve cleaved embryos developing to morula stage. This is the first in vitro embryo production attempt in sei and Bryde's whales.

Predictive variables of in vitro fertilization and pre-implantation embryo development in the mouse

2002 ◽  
Vol 63 (1) ◽  
pp. 38-46 ◽  
Author(s):  
Juan J. TarÍn ◽  
Vanessa Gómez-Piquer ◽  
Sonia PÉrez-AlbalÁ ◽  
Carlos Hermenegildo ◽  
Antonio Cano
Keyword(s):  
In Vitro Fertilization ◽  
Embryo Development ◽  
Vitro Fertilization ◽  
Predictive Variables

Comparison of results obtained with human serum and a protein solution as a supplement for in vitro fertilization culture medium

1992 ◽  
Vol 58 (3) ◽  
pp. 637-639 ◽  
Author(s):  
Gerritdina J. Huisman ◽  
Nadia M. Lo-A-Njoe ◽  
Albert Th. Alberda ◽  
Robert A. Leerentveld ◽  
Arie Verhoeff ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Human Serum ◽  
Culture Medium ◽  
Protein Solution ◽  
Vitro Fertilization ◽  
Comparison Of Results

Effect of Astragalus polysaccharide addition to thawed boar sperm on in vitro fertilization and embryo development

2018 ◽  
Vol 121 ◽  
pp. 21-26 ◽  
Author(s):  
Xiao-gang Weng ◽  
Ming-ming Cai ◽  
Yu-ting Zhang ◽  
Yan Liu ◽  
Zheng-ling Gao ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Embryo Development ◽  
Vitro Fertilization ◽  
Astragalus Polysaccharide ◽  
Boar Sperm

221 EFFECT OF ROCKING ON IN VITRO PRODUCTION OF BOVINE EMBRYOS

2009 ◽  
Vol 21 (1) ◽  
pp. 209
Author(s):  
Y. Serita ◽  
C. Kubota ◽  
T. Kojima
Keyword(s):  
In Vitro Fertilization ◽  
Embryo Development ◽  
Developmental Competence ◽  
In Vitro Production ◽  
Humid Atmosphere ◽  
Vitro Fertilization ◽  
Rate Of Development ◽  
Fetal Bovine ◽  
Vitro Production

This study tested whether embryo development yield using in vitro fertilization (IVF) could be improved by rocking cultures. Bovine ovaries were obtained at a slaughterhouse and transported to the laboratory within 6 h. Cumulus–oocyte complexes were collected and 20–25 were transferred in 100-μL drops of TCM-199 containing 10% fetal bovine serum and antibiotics under paraffin oil. Maturation was for 20–24 h at 38.5°C under 5% CO2 and 95% air in a humid atmosphere (IVM). In vitro fertilization was carried out for 6 h using frozen–thawed sperm from a single bull in modified Brackett and Oliphant (BO) medium. Presumptive zygotes were cultured in CR1aa supplemented with 10 mg mL–1 of BSA or 5% FBS for 9 d at 38.5°C under 5% CO2, 5% O2, and 90% N2 in a humid atmosphere (IVC). Rocking was performed to a height of 6 cm every 7 s using a Profile Rocker (New Brunswick Scientific Co., Edison, NJ, USA) in an incubator. Dishes were placed at a 15-cm distance from the fulcrum of the rocker. The conventional method (no rocking) served as a control, and every experiment was replicated 3 times. For Experiment 1, the effect of the period of rocking on developmental competence was examined when COC or zygotes were subjected to rocking for IVM, IVF, or IVC (IVM-move, IVF-move, and IVC-move). There were no significant differences in rates of oocyte maturation, cleavage, and development for IVM-move v. the control, or for rate of development between IVC-move and the control. However, the rate of fertilization for IVF-move was higher than that of the control (88.9 v. 67.5%; P < 0.01), and the rate of development was higher for IVF-move than for the control (39.0 v. 25.7%; P < 0.05). For Experiment 2, the effect of rocking frequency during IVF on development was determined. The IVF cultures were rocked every 7, 3.5, and 1.5 s (IVF-1move, IVF-2move, IVF-3move). The rates of cleavage on IVF-1move, IVF-2move, IVF-3move, and the control were 74.3, 69.8, 68.8, and 60.4%, and the rates of development were 39.0, 48.3, 26.2, and 25.7%, respectively. The rates of development on IVF-1move and IVF-2move were significantly different from the control and IVF-3move (P < 0.01). These results showed that rocking during IVF improved fertilization and embryo yield, and that frequency of rocking affected embryo development.

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