Biological characterization and optical imaging of brain-derived neurotrophic factor-green fluorescent protein suggest an activity-dependent local release of brain-derived neurotrophic factor in neurites of cultured hippocampal neurons

2001 ◽  
Vol 64 (1) ◽  
pp. 1-10 ◽  
Author(s):  
Masami Kojima ◽  
Nobuyuki Takei ◽  
Tadahiro Numakawa ◽  
Yasuyuki Ishikawa ◽  
Shingo Suzuki ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
Neurotrophic Factor ◽  
Hippocampal Neurons ◽  
Fluorescent Protein ◽  
Brain Derived Neurotrophic Factor ◽  
Biological Characterization ◽  
Green Fluorescent ◽  
Local Release ◽  
Activity Dependent ◽  
Cultured Hippocampal Neurons

scholarly journals A neuron-specific cytoplasmic dynein isoform preferentially transports TrkB signaling endosomes

2008 ◽  
Vol 181 (6) ◽  
pp. 1027-1039 ◽  
Author(s):  
Junghoon Ha ◽  
Kevin W.-H. Lo ◽  
Kenneth R. Myers ◽  
Tiffany M. Carr ◽  
Michael K. Humsi ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
Receptor Tyrosine Kinase ◽  
Pc12 Cell ◽  
Hippocampal Neurons ◽  
Fluorescent Protein ◽  
Cytoplasmic Dynein ◽  
Neurotrophin Receptor ◽  
Immunoaffinity Purification ◽  
Green Fluorescent ◽  
Cultured Hippocampal Neurons

Cytoplasmic dynein is the multisubunit motor protein for retrograde movement of diverse cargoes to microtubule minus ends. Here, we investigate the function of dynein variants, defined by different intermediate chain (IC) isoforms, by expressing fluorescent ICs in neuronal cells. Green fluorescent protein (GFP)–IC incorporates into functional dynein complexes that copurify with membranous organelles. In living PC12 cell neurites, GFP–dynein puncta travel in both the anterograde and retrograde directions. In cultured hippocampal neurons, neurotrophin receptor tyrosine kinase B (TrkB) signaling endosomes are transported by cytoplasmic dynein containing the neuron-specific IC-1B isoform and not by dynein containing the ubiquitous IC-2C isoform. Similarly, organelles containing TrkB isolated from brain by immunoaffinity purification also contain dynein with IC-1 but not IC-2 isoforms. These data demonstrate that the IC isoforms define dynein populations that are selectively recruited to transport distinct cargoes.

scholarly journals A New Mouse Line Reporting the Translation of Brain-Derived Neurotrophic Factor Using Green Fluorescent Protein

eNeuro ◽  
2019 ◽  
Vol 7 (1) ◽  
pp. ENEURO.0462-19.2019 ◽  
Author(s):  
Erin Wosnitzka ◽  
Xinsheng Nan ◽  
Jeff Nan ◽  
Pedro Chacón-Fernández ◽  
Lothar Kussmaul ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
Neurotrophic Factor ◽  
Fluorescent Protein ◽  
Brain Derived Neurotrophic Factor ◽  
Mouse Line ◽  
Green Fluorescent

NRSF Causes cAMP-Sensitive Suppression of Sodium Current in Cultured Hippocampal Neurons

2002 ◽  
Vol 88 (1) ◽  
pp. 409-421 ◽  
Author(s):  
H. Nadeau ◽  
H. A. Lester
Keyword(s):  
Hippocampal Neurons ◽  
Fluorescent Protein ◽  
Sodium Current ◽  
Striking Similarity ◽  
Secondary Effects ◽  
Functional Effect ◽  
Green Fluorescent ◽  
Altered Sensitivity ◽  
Cultured Hippocampal Neurons

The neuron restrictive silencer factor (NRSF/REST) has been shown to bind to the promoters of many neuron-specific genes and is able to suppress transcription of Na+channels in PC12 cells, although its functional effect in terminally differentiated neurons is unknown. We constructed lentiviral vectors to express NRSF as a bicistronic message with green fluorescent protein (GFP) and followed infected hippocampal neurons in culture over a period of 1–2 wk. NRSF-expressing neurons showed a time-dependent suppression of Na+channel function as measured by whole cell electrophysiology. Suppression was reversed or prevented by the addition of membrane-permeable cAMP analogues and enhanced by cAMP antagonists but not affected by increasing protein expression with a viral enhancer. Secondary effects, including altered sensitivity to glutamate and GABA and reduced outward K+currents, were duplicated by culturing GFP-infected control neurons in TTX. The striking similarity of the phenotypes makes NRSF potentially useful as a genetic “silencer” and also suggests avenues of further exploration that may elucidate the transcription factor's in vivo role in neuronal plasticity.

scholarly journals Brain-Derived Neurotrophic Factor Promotes the Maturation of GABAergic Mechanisms in Cultured Hippocampal Neurons

2002 ◽  
Vol 22 (17) ◽  
pp. 7580-7585 ◽  
Author(s):  
Maki K. Yamada ◽  
Kohsuke Nakanishi ◽  
Shizu Ohba ◽  
Takeshi Nakamura ◽  
Yuji Ikegaya ◽  
...  
Keyword(s):  
Neurotrophic Factor ◽  
Hippocampal Neurons ◽  
Brain Derived Neurotrophic Factor ◽  
Cultured Hippocampal Neurons

scholarly journals Introduction of Green Fluorescent Protein (GFP) into Hippocampal Neurons through Viral Infection

2010 ◽  
Vol 2010 (4) ◽  
pp. pdb.prot5406-pdb.prot5406 ◽  
Author(s):  
R. Malinow ◽  
Y. Hayashi ◽  
M. Maletic-Savatic ◽  
S. H. Zaman ◽  
J.-C. Poncer ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
Viral Infection ◽  
Hippocampal Neurons ◽  
Fluorescent Protein ◽  
Green Fluorescent
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