Functional expression of distinct NMDA channel subunits tagged with green fluorescent protein in hippocampal neurons in culture

2002 ◽  
Vol 42 (3) ◽  
pp. 306-318 ◽  
Author(s):  
J.-H. Luo ◽  
Z.Y. Fu ◽  
G. Losi ◽  
B.G. Kim ◽  
K. Prybylowski ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
Hippocampal Neurons ◽  
Fluorescent Protein ◽  
Functional Expression ◽  
Nmda Channel ◽  
Green Fluorescent

scholarly journals A neuron-specific cytoplasmic dynein isoform preferentially transports TrkB signaling endosomes

2008 ◽  
Vol 181 (6) ◽  
pp. 1027-1039 ◽  
Author(s):  
Junghoon Ha ◽  
Kevin W.-H. Lo ◽  
Kenneth R. Myers ◽  
Tiffany M. Carr ◽  
Michael K. Humsi ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
Receptor Tyrosine Kinase ◽  
Pc12 Cell ◽  
Hippocampal Neurons ◽  
Fluorescent Protein ◽  
Cytoplasmic Dynein ◽  
Neurotrophin Receptor ◽  
Immunoaffinity Purification ◽  
Green Fluorescent ◽  
Cultured Hippocampal Neurons

Cytoplasmic dynein is the multisubunit motor protein for retrograde movement of diverse cargoes to microtubule minus ends. Here, we investigate the function of dynein variants, defined by different intermediate chain (IC) isoforms, by expressing fluorescent ICs in neuronal cells. Green fluorescent protein (GFP)–IC incorporates into functional dynein complexes that copurify with membranous organelles. In living PC12 cell neurites, GFP–dynein puncta travel in both the anterograde and retrograde directions. In cultured hippocampal neurons, neurotrophin receptor tyrosine kinase B (TrkB) signaling endosomes are transported by cytoplasmic dynein containing the neuron-specific IC-1B isoform and not by dynein containing the ubiquitous IC-2C isoform. Similarly, organelles containing TrkB isolated from brain by immunoaffinity purification also contain dynein with IC-1 but not IC-2 isoforms. These data demonstrate that the IC isoforms define dynein populations that are selectively recruited to transport distinct cargoes.

scholarly journals Introduction of Green Fluorescent Protein (GFP) into Hippocampal Neurons through Viral Infection

2010 ◽  
Vol 2010 (4) ◽  
pp. pdb.prot5406-pdb.prot5406 ◽  
Author(s):  
R. Malinow ◽  
Y. Hayashi ◽  
M. Maletic-Savatic ◽  
S. H. Zaman ◽  
J.-C. Poncer ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
Viral Infection ◽  
Hippocampal Neurons ◽  
Fluorescent Protein ◽  
Green Fluorescent
Sign in / Sign up

Export Citation Format

Share Document