Calcium signal-mediated expression of the vasoactive intestinal polypeptide gene and its small contribution to activity-dependent survival of mouse cerebellar granule cells

2004 ◽  
Vol 77 (1) ◽  
pp. 26-34 ◽  
Author(s):  
Mamoru Fukuchi ◽  
Saori Sakuragawa ◽  
Akiko Tabuchi ◽  
Masaaki Tsuda
Keyword(s):  
Vasoactive Intestinal Polypeptide ◽  
Granule Cells ◽  
Cerebellar Granule Cells ◽  
Calcium Signal ◽  
Small Contribution ◽  
Cerebellar Granule ◽  
Activity Dependent ◽  
Intestinal Polypeptide

scholarly journals The Expression of the PDZ Protein MALS-1/Velis Is Regulated by Calcium and Calcineurin in Cerebellar Granule Cells

2002 ◽  
Vol 277 (51) ◽  
pp. 49585-49590 ◽  
Author(s):  
Bastiano Sanna ◽  
Dana Kramer ◽  
Armando A. Genazzani
Keyword(s):  
Gene Expression ◽  
Neuronal Development ◽  
Granule Cells ◽  
De Novo ◽  
Cerebellar Granule Cells ◽  
Transcriptional Level ◽  
Synaptic Density ◽  
Cerebellar Granule ◽  
Activity Dependent ◽  
Post Synaptic Density

Activity-dependent gene expression is thought to be important in shaping neuronal development and in modifying the protein content of neurons. Ca2+entry into neurons appears to be one of the key effectors of activity-dependent gene expression. Among the possible downstream targets of calcium, the protein phosphatase calcineurin represents a prime candidate. We hereby report that in cultured cerebellar granule cells the activation of the Ca2+/calcineurin pathway via either voltage- or ligand- operated Ca2+channels regulates MALS-1 and MALS-2 expression at the transcriptional level. These proteins are integral parts of the post-synaptic density and are also involved in receptor trafficking. MALS regulation is not at the level of mRNA stability and does not requirede novoprotein synthesis, thereby suggesting a direct pathway. These data suggest that Ca2+entry by means of calcineurin is capable of controlling the structure of the post-synaptic density by controlling the expression of key components at the transcriptional level.

Involvement of endogenous PACAP expression in the activity-dependent survival of mouse cerebellar granule cells

2001 ◽  
Vol 39 (1) ◽  
pp. 85-93 ◽  
Author(s):  
Akiko Tabuchi ◽  
Motoko Koizumi ◽  
Jun Nakatsubo ◽  
Takahiro Yaguchi ◽  
Masaaki Tsuda
Keyword(s):  
Granule Cells ◽  
Cerebellar Granule Cells ◽  
Cerebellar Granule ◽  
Activity Dependent
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