scholarly journals Growth factors in human serum during operative tibial lengthening with the ilizarov method

2013 ◽  
Vol 31 (12) ◽  
pp. 1966-1970 ◽  
Author(s):  
Maksim V. Stogov ◽  
Svetlana N. Luneva ◽  
Konstantin I. Novikov
Keyword(s):  
Growth Factors ◽  
Human Serum ◽  
Ilizarov Method ◽  
Tibial Lengthening

Growth factors of the TGF-b family in blood of achondroplasia patients at stages of orthopedic treatment

2018 ◽  
pp. 70-76
Author(s):  
Е.П. Выхованец ◽  
Н.В. Сакулин ◽  
С.Н. Лунева ◽  
Н.В. Накоскина ◽  
О.В. Климов
Keyword(s):  
Growth Factors ◽  
Limb Lengthening ◽  
Serum Levels ◽  
Long Bone ◽  
Ilizarov Method ◽  
Bone Lengthening ◽  
Serum Concentrations ◽  
Orthopedic Treatment ◽  
Tibial Lengthening ◽  
Height Increase

Наиболее важной задачей при дистракционном остеосинтезе является создание простых и эффективных способов контроля темпов дистракции позволяющих производить удлинение конечности в оптимальном режиме у различных групп пациентов. Цель исследования - анализ содержания сывороточных концентраций TGFb-1 и TGFb-2, BMP-4 и BMP-6 у лиц при увеличении роста с косметической целью и у пациентов с ахондроплазией на этапах дистракционного остеогенеза при удлинении голени методом Илизарова. Методика. Определение ростовых факторов проводили на комплексе оборудования фирмы Thermofisher (США): детектор Multiscan FC, встряхиватель iEMS, автоматический промыватель планшет WellWash. Для измерения концентрации факторов в сыворотке крови использовали наборы для иммуноферментного анализа (ИФА) eBioscience и RayBiotech Inc. (США). Результаты . Показано, что в сыворотке крови людей с ахондроплазией, еще до какого-либо ортопедического лечения концентрации TGF-b2 и ВМР-4, ниже, а TGF-b1 и ВМР-6, выше, чем концентрации этих факторов у людей при увеличении роста с косметической целью. У последних удлинение конечностей сопровождается возрастанием содержания в сыворотке крови факторов TGF-b1 и TGF-b2 на этапах начала и середины процесса дистракции. У пациентов с ахондроплазией, наблюдается обратная динамика: концентрация данных факторов (ВМР-4 и ВМР-3) в крови ахондропатов к концу дистракции увеличивается в 3,5 и 2 раза соответственно, снижаясь в периоде фиксации. Заключение. Таким образом, у пациентов с ахондроплазией происходит нарушение этапности процессов костного ремоделирования. Elaboration of simple and effective ways for managing the distraction rate to provide an optimum regimen of limb lengthening for different groups of patients is an important task in implementation of transosseous osteosynthesis. The aim of this study was to analyze blood serum concentrations of TGFb-1, TGFb-2, BMP-4, and BMP-6 in individuals undergoing cosmetic height increase and patients with achondroplasia at different stages of distraction osteogenesis in tibial lengthening using the Ilizarov method. Methods. Concentrations of growth factors were measured using a set of Thermofisher (USA) equipment, including a Multiscan FC detector, iEMS Shaker, and automatic WellWash Washer and ELISA kits (eBioscience and RayBiotech Inc., USA). Results. Serum concentrations of TGFb-2 and BMP-4 were lower and TGFb-1 and BMP-6 were higher in achondroplasia patients than in individuals with cosmetic height increase even before any orthopedic treatment. Long bone lengthening for cosmetic height increase was associated with increases in serum levels of TGFb-1 and TGFb-2 at the start and in the middle of distraction. In achondroplasia patients, opposite changes were observed; serum concentrations of BMP-4 and BMP-3 increased 3.5 and 2 times, respectively, by the end of distraction and decreased during fixation. Conclusion. Therefore, we observed a disorder of the stage-by-stage bone remodeling process in achondroplasia patients.

Electromyographic and Nerve Conduction Changes After Tibial Lengthening by the Ilizarov Method

1993 ◽  
Vol 13 (4) ◽  
pp. 473-477 ◽  
Author(s):  
N. L. Young ◽  
R. J. Davis ◽  
D. F. Bell ◽  
D. M. Redmond
Keyword(s):  
Nerve Conduction ◽  
Ilizarov Method ◽  
Tibial Lengthening

scholarly journals Proteolytic conversion of insulin-like growth factors to an acidic form(s)

1984 ◽  
Vol 223 (1) ◽  
pp. 97-103 ◽  
Author(s):  
A D Kuffer ◽  
A C Herington
Keyword(s):  
Growth Factors ◽  
Human Serum ◽  
Gel Filtration ◽  
Amino Acid Sequences ◽  
Full Spectrum ◽  
Acidic Form ◽  
Igf I ◽  
Acid Gel ◽  
Insulin Like Growth Factors

The relative amounts of the various forms of bioassayable insulin-like growth factors (IGF) isolated from human serum or serum fraction Cohn IV-1 depend on the purification procedure. With acid gel filtration or acid/ethanol extraction as the initial step, IGF-II (pI approximately 6.5) was the most abundant (40-70%) followed by somatomedin A (pI approximately 7.4; 15-23%), an acidic form of insulin-like activity (ILA pI 4.8) (13-21%) and IGF-I (pI approximately 8.5; 5-27%). If, however, pH 5.5 ion-exchange chromatography on SP-Sephadex was used prior to acid gel filtration, the acidic pI 4.8 form was the major (greater than 90%) species recovered and was accompanied by a quantitative loss of the other IGF species. This suggested a possible conversion of IGF-I, somatomedin A and/or IGF-II to the acidic ILA pI 4.8 form(s) during the SP-Sephadex procedure. Further experiments indicated that differences in the yields of ILA pI 4.8 were not due simply to differences in the initial pH conditions of the various methods (i.e. acid versus neutral), although exposure to pH 9.7 (a pH experienced during elution of IGF activity from the SP-Sephadex) did appear to play a role. The involvement of the carrier protein in the conversion process was tested by subjecting carrier-free IGF-I and IGF-II to the SP-Sephadex procedure. No conversion of the free forms to ILA pI 4.8 occurred. To examine the possible role of proteinase in the conversion of IGFs to ILA pI 4.8, SP-Sephadex chromatography was performed in the presence of a broad spectrum proteinase inhibitor. The IGF distribution pattern obtained closely resembled the ‘normal’ pattern seen with acid gel filtration, indicating that proteinase inactivation had prevented conversion to ILA pI 4.8. These data suggest that proteolytic conversion of IGF-I, somatomedin A and IGF-II to more acidic ILA pI 4.8 form(s) (i) occurs during SP-Sephadex chromatography, (ii) is not prevented simply by prior acid exposure, and (iii) takes place only when IGF-I and -II are in their high-Mr carrier-bound forms. Since IGF-I and IGF-II, although homologous, have unique amino acid sequences, the conversion of both IGFs implies that at least two acidic ILA forms exist. Nevertheless, because ILA pI 4.8 retains the full spectrum of IGF bioactivities in vitro, and significant quantities are present in normal human serum (21%), it would suggest that proteolytic conversion of IGF-I, somatomedin A and IGF-II to ILA pI 4.8 in vivo may be a physiologically significant event.

Simultaneous Ipsilateral Femoral and Tibial Lengthening with the Ilizarov Method

1999 ◽  
Vol 19 (3) ◽  
pp. 386-390 ◽  
Author(s):  
Andrew R. Curran ◽  
Ken N. Kuo ◽  
John P. Lubicky
Keyword(s):  
Ilizarov Method ◽  
Tibial Lengthening

scholarly journals Free insulin-like growth factors (IGF-I and IGF-II) in human serum

FEBS Letters ◽  
1994 ◽  
Vol 348 (2) ◽  
pp. 185-191 ◽  
Author(s):  
Jan Frystyk ◽  
Christian Skjaerbaek ◽  
Bo Dinesen ◽  
Hans Ørskov
Keyword(s):  
Growth Factors ◽  
Human Serum ◽  
Free Insulin ◽  
Igf I ◽  
Insulin Like Growth Factors

Growth of 9·5-day rat embryos in human serum

Development ◽  
1983 ◽  
Vol 76 (1) ◽  
pp. 1-8
Author(s):  
M. Gupta ◽  
F. Beck
Keyword(s):  
Growth Factors ◽  
Human Serum ◽  
Dna Content ◽  
Rat Serum ◽  
Rat Embryos ◽  
Embryonic Growth ◽  
Essential Growth

Rat embryos were explanted at 9·5 days and cultured for 48 h in human serum supplemented with glucose. The embryos were anaemic and frequently malformed. The haemoglobin and DNA content of these embryos was less than those grown in pure rat serum. Addition of 10% rat serum improved the embryonic growth as well as the haemoglobin and DNA content. This suggests that rat serum provides essential growth factors required by the embryos which are not present in human serum.

CLINICAL AND RADIOGRAPHIC FEATURES OF REGENERATE FORMATION IN CASES OF TIBIAL LENGTHENING BY ILIZAROV METHOD

2018 ◽  
Vol 3 ◽  
pp. 5-9
Author(s):  
A.A. Artemiev ◽  
◽  
A.A. Gritsyuk ◽  
N.N. Nelin ◽  
J.S. Solovyov ◽  
...  
Keyword(s):  
Ilizarov Method ◽  
Radiographic Features ◽  
Tibial Lengthening
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