The Authors’ Reply: Dynamic liver graft preservation in cDCD: What is the best fit?

2021 ◽  
Author(s):  
Patricia Ruiz ◽  
Andres Valdivieso ◽  
Ibone Palomares ◽  
Mikel Prieto ◽  
Alberto Ventoso ◽  
...  
Keyword(s):  
Liver Graft ◽  
Graft Preservation ◽  
Best Fit

Dynamic liver graft preservation in cDCD: What is the best fit?

2021 ◽  
Author(s):  
Xavier Muller ◽  
Guillaume Rossignol ◽  
Kayvan Mohkam ◽  
Mickaël Lesurtel ◽  
Jean‐Yves Mabrut
Keyword(s):  
Liver Graft ◽  
Graft Preservation ◽  
Best Fit

scholarly journals Role of PEG35, Mitochondrial ALDH2, and Glutathione in Cold Fatty Liver Graft Preservation: An IGL-2 Approach

2021 ◽  
Vol 22 (10) ◽  
pp. 5332
Author(s):  
Raquel G. Bardallo ◽  
Rui Teixeira da Silva ◽  
Teresa Carbonell ◽  
Emma Folch-Puy ◽  
Carlos Palmeira ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Fatty Liver ◽  
Liver Graft ◽  
Suitable Alternative ◽  
Graft Preservation ◽  
Preservation Solutions ◽  
Microcirculatory Disturbances ◽  
Organ Recovery ◽  
Surgical Manipulations

The total damage inflicted on the liver before transplantation is associated with several surgical manipulations, such as organ recovery, washout of the graft, cold conservation in organ preservation solutions (UW, Celsior, HTK, IGL-1), and rinsing of the organ before implantation. Polyethylene glycol 35 (PEG35) is the oncotic agent present in the IGL-1 solution, which is an alternative to UW and Celsior solutions in liver clinical transplantation. In a model of cold preservation in rats (4 °C; 24 h), we evaluated the effects induced by PEG35 on detoxifying enzymes and nitric oxide, comparing IGL-1 to IGL-0 (which is the same as IGL-1 without PEG). The benefits were also assessed in a new IGL-2 solution characterized by increased concentrations of PEG35 (from 1 g/L to 5 g/L) and glutathione (from 3 mmol/L to 9 mmol/L) compared to IGL-1. We demonstrated that PEG35 promoted the mitochondrial enzyme ALDH2, and in combination with glutathione, prevented the formation of toxic aldehyde adducts (measured as 4-hydroxynonenal) and oxidized proteins (AOPP). In addition, PEG35 promoted the vasodilator factor nitric oxide, which may improve the microcirculatory disturbances in steatotic grafts during preservation and revascularization. All of these results lead to a reduction in damage inflicted on the fatty liver graft during the cold storage preservation. In this communication, we report on the benefits of IGL-2 in hypothermic static preservation, which has already been proved to confer benefits in hypothermic oxygenated dynamic preservation. Hence, the data reported here reinforce the fact that IGL-2 is a suitable alternative to be used as a unique solution/perfusate when hypothermic static and preservation strategies are used, either separately or combined, easing the logistics and avoiding the mixture of different solutions/perfusates, especially when fatty liver grafts are used. Further research regarding new therapeutic and pharmacological insights is needed to explore the underlying mitochondrial mechanisms exerted by PEG35 in static and dynamic graft preservation strategies for clinical liver transplantation purposes.

Liver graft preservation using perfluorocarbon improves the outcomes of simulated donation after cardiac death liver transplantation in rats

2017 ◽  
Vol 23 (9) ◽  
pp. 1171-1185 ◽  
Author(s):  
Shinya Okumura ◽  
Tadahiro Uemura ◽  
Xiangdong Zhao ◽  
Yuki Masano ◽  
Tatsuaki Tsuruyama ◽  
...  
Keyword(s):  
Liver Transplantation ◽  
Cardiac Death ◽  
Liver Graft ◽  
Donation After Cardiac Death ◽  
Graft Preservation

The of Institute George Lopez (IGL-1) Solution in Liver Graft Preservation - Experience With 100 Liver Transpant in a Single Center.

2014 ◽  
Vol 98 ◽  
pp. 707
Author(s):  
J. Wiederkehr ◽  
M. Igreja ◽  
M. Nogara ◽  
N. Goncalves ◽  
M. Godoy ◽  
...  
Keyword(s):  
Liver Graft ◽  
Single Center ◽  
Graft Preservation

AMPK involvement in endoplasmic reticulum stress and autophagy modulation after fatty liver graft preservation: a role for melatonin and trimetazidine cocktail

2013 ◽  
Vol 55 (1) ◽  
pp. 65-78 ◽  
Author(s):  
Mohamed Amine Zaouali ◽  
Eleonora Boncompagni ◽  
Russel J. Reiter ◽  
Mohamed Bejaoui ◽  
Isabel Freitas ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Endoplasmic Reticulum Stress ◽  
Fatty Liver ◽  
Liver Graft ◽  
Graft Preservation

scholarly journals Polyethylene Glycol 35 as a Perfusate Additive for Mitochondrial and Glycocalyx Protection in HOPE Liver Preservation

2020 ◽  
Vol 21 (16) ◽  
pp. 5703 ◽  
Author(s):  
Arnau Panisello Rosello ◽  
Rui Teixeira da Silva ◽  
Carlos Castro ◽  
Raquel G. Bardallo ◽  
Maria Calvo ◽  
...  
Keyword(s):  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Graft Function ◽  
Water Soluble ◽  
Liver Graft ◽  
Liver Preservation ◽  
Hypothermic Preservation ◽  
Graft Preservation ◽  
Abdominal Organs ◽  
Good Target

Organ transplantation is a multifactorial process in which proper graft preservation is a mandatory step for the success of the transplantation. Hypothermic preservation of abdominal organs is mostly based on the use of several commercial solutions, including UW, Celsior, HTK and IGL-1. The presence of the oncotic agents HES (in UW) and PEG35 (in IGL-1) characterize both solution compositions, while HTK and Celsior do not contain any type of oncotic agent. Polyethylene glycols (PEGs) are non-immunogenic, non-toxic and water-soluble polymers, which present a combination of properties of particular interest in the clinical context of ischemia-reperfusion injury (IRI): they limit edema and nitric oxide induction and modulate immunogenicity. Besides static cold storage (SCS), there are other strategies to preserve the organ, such as the use of machine perfusion (MP) in dynamic preservation strategies, which increase graft function and survival as compared to the conventional static hypothermic preservation. Here we report some considerations about using PEG35 as a component of perfusates for MP strategies (such as hypothermic oxygenated perfusion, HOPE) and its benefits for liver graft preservation. Improved liver preservation is closely related to mitochondria integrity, making this organelle a good target to increase graft viability, especially in marginal organs (e.g., steatotic livers). The final goal is to increase the pool of suitable organs, and thereby shorten patient waiting lists, a crucial problem in liver transplantation.

An Oxygenated Perfluorocarbon Emulsion Improves Liver Graft Preservation Evaluated in DCD livers of Male Sprague Dawley Rats

2021 ◽  
Author(s):  
Guoyi Wu ◽  
Yu Liu ◽  
Rui Chen ◽  
Shanshan Zhan ◽  
Jun Wang ◽  
...  
Keyword(s):  
Liver Graft ◽  
Sprague Dawley ◽  
Perfluorocarbon Emulsion ◽  
Graft Preservation ◽  
Sprague Dawley Rats

Sirtuin 1 in Steatotic and Non Steatotic Liver Graft Preservation: Differential Aspects.

2014 ◽  
Vol 98 ◽  
pp. 345
Author(s):  
M. Bejaoui ◽  
E. Pantazi ◽  
Amine M. Zaouali ◽  
E. Folch-Puy ◽  
F. Bardag-Gorce ◽  
...  
Keyword(s):  
Sirtuin 1 ◽  
Liver Graft ◽  
Steatotic Liver ◽  
Graft Preservation

scholarly journals Relevance of proteolysis and proteasome activation in fatty liver graft preservation: An Institut Georges Lopez-1vsUniversity of Wisconsin appraisal

2017 ◽  
Vol 23 (23) ◽  
pp. 4211 ◽  
Author(s):  
Mohamed Amine Zaouali ◽  
Arnau Panisello-Roselló ◽  
Alexandre Lopez ◽  
Carlos Castro Benítez ◽  
Emma Folch-Puy ◽  
...  
Keyword(s):  
Fatty Liver ◽  
Liver Graft ◽  
Graft Preservation
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