Limbal Bio‐engineered Tissue Employing 3D Nanofiber‐Aerogel Scaffold to Facilitate LSCs Growth and Migration

After experimetnal damage to the retina with a variety of procedures Müller cell hypertrophy and migration occurs. According to Kuwabara and others the reactive process in these injuries is evidenced by a marked increase in amount of glycogen in the Müller cells. These cells were considered originally supporting elements with fiber processes extending throughout the retina from inner limiting membrane to external limiting membrane, but are known now to have high lactic acid dehydrogenase activity and the ability to synthesize glycogen. Since the periodic acid-chromic acid-silver methenamine technique was shown to demonstrate glycogen at the electron microscope level, it was selected to react with glycogen in the fine processes of the Müller cell that ramify among the neural elements in various layers of the retina and demarcate these cells cytologically. The Rhesus monkey was chosen as an example of a well vascularized retina and the rabbit as an example of a avascular retina to explore the possibilities of the technique.

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Structure and migration of planar defects in crystals observed in atomic scale

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100108568 ◽

1978 ◽

Vol 36 (1) ◽

pp. 284-285 ◽

Cited By ~ 1

Author(s):

H. Hashimoto ◽

Y. Sugimoto ◽

Y. Takai ◽

H. Endoh

Keyword(s):

Electron Microscope ◽

Rotation Angle ◽

Crystal Surface ◽

Electron Gun ◽

Atomic Scale ◽

Present Observation ◽

Twist Boundary ◽

Optical Magnification ◽

Zinc Crystal ◽

And Migration

As was demonstrated by the present authors that atomic structure of simple crystal can be photographed by the conventional 100 kV electron microscope adjusted at “aberration free focus (AFF)” condition. In order to operate the microscope at AFF condition effectively, highly stabilized electron beams with small energy spread and small beam divergence are necessary. In the present observation, a 120 kV electron microscope with LaB6 electron gun was used. The most of the images were taken with the direct electron optical magnification of 1.3 million times and then magnified photographically.1. Twist boundary of ZnSFig. 1 is the image of wurtzite single crystal with twist boundary grown on the surface of zinc crystal by the reaction of sulphur vapour of 1540 Torr at 500°C. Crystal surface is parallel to (00.1) plane and electron beam is incident along the axis normal to the crystal surface. In the twist boundary there is a dislocation net work between two perfect crystals with a certain rotation angle.

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