scholarly journals Incorporating peptide aptamers into resistive pulse sensing

2020 ◽  
Vol 3 (1) ◽  
Author(s):  
Rhushabh Maugi ◽  
Zarina Salkenova ◽  
Mark Platt
Keyword(s):  
Peptide Aptamers ◽  
Resistive Pulse ◽  
Resistive Pulse Sensing

scholarly journals Incorporating Peptide Aptamers into Resistive Pulse Sensing

2019 ◽  
Author(s):  
Mark Platt ◽  
Rhushabh Maugi
Keyword(s):  
Electrophoretic Mobility ◽  
Dna Aptamer ◽  
Peptide Sequence ◽  
Peptide Aptamers ◽  
Reactive Protein ◽  
Simple Strategy ◽  
Resistive Pulse ◽  
Universal Approach ◽  
Resistive Pulse Sensing ◽  
Phosphate Groups

<p>The use of nanocarriers within resistive pulse sensing, RPS, aids the detection and quantification of analytes. In the absence of convection, the signal strength and frequency can dependent upon the electrophoretic mobility of the nanocarrier/ analyte. Here we have developed a simple strategy to incorporate peptide aptamers onto RPS assays with enhanced electrophoretic signals. Using a hybrid DNA-Peptide nanocarrier an existing peptide was incorporated into a rapid assay without having to engineer or modify the peptide sequence. The surface of a nanocarrier is coated with a mixture of peptide aptamers and a nonbinding DNA. The binding of the target to the peptide creates an “analyte corona” which shields the phosphate groups of the underlying DNA. This results in a change in electrophoretic mobility of the nanocarrier. The signal is concentration dependent and is illustrated using a peptide to a key biomarker of infection, C-Reactive Protein, CRP. As a comparison we also show the binding of the CRP to a DNA aptamer. This universal approach can be easily adapted to other peptides without the peptide itself to undergo any chemical modifications opening new opportunities and applications in RPS strategies. </p>

scholarly journals Incorporating Peptide Aptamers into Resistive Pulse Sensing

2019 ◽  
Author(s):  
Mark Platt ◽  
Rhushabh Maugi
Keyword(s):  
Electrophoretic Mobility ◽  
Dna Aptamer ◽  
Peptide Sequence ◽  
Peptide Aptamers ◽  
Reactive Protein ◽  
Simple Strategy ◽  
Resistive Pulse ◽  
Universal Approach ◽  
Resistive Pulse Sensing ◽  
Phosphate Groups

<p>The use of nanocarriers within resistive pulse sensing, RPS, aids the detection and quantification of analytes. In the absence of convection, the signal strength and frequency can dependent upon the electrophoretic mobility of the nanocarrier/ analyte. Here we have developed a simple strategy to incorporate peptide aptamers onto RPS assays with enhanced electrophoretic signals. Using a hybrid DNA-Peptide nanocarrier an existing peptide was incorporated into a rapid assay without having to engineer or modify the peptide sequence. The surface of a nanocarrier is coated with a mixture of peptide aptamers and a nonbinding DNA. The binding of the target to the peptide creates an “analyte corona” which shields the phosphate groups of the underlying DNA. This results in a change in electrophoretic mobility of the nanocarrier. The signal is concentration dependent and is illustrated using a peptide to a key biomarker of infection, C-Reactive Protein, CRP. As a comparison we also show the binding of the CRP to a DNA aptamer. This universal approach can be easily adapted to other peptides without the peptide itself to undergo any chemical modifications opening new opportunities and applications in RPS strategies. </p>

Surface modification to suppress small pore clogging in resistive pulse sensing

2020 ◽  
Vol 13 (11) ◽  
pp. 115002
Author(s):  
Yukichi Horiguchi ◽  
Yuji Miyahara
Keyword(s):  
Surface Modification ◽  
Resistive Pulse ◽  
Small Pore ◽  
Resistive Pulse Sensing ◽  
Pore Clogging

Role of Nanopore Geometry in Particle Resolution by Resistive‐Pulse Sensing

2021 ◽  
Vol 6 (1) ◽  
pp. 59-67
Author(s):  
Durdane Yilmaz ◽  
Dila Kaya ◽  
Kaan Kececi ◽  
Ali Dinler
Keyword(s):  
Resistive Pulse ◽  
Resistive Pulse Sensing

scholarly journals High-Resolution Single Particle Zeta Potential Characterisation of Biological Nanoparticles using Tunable Resistive Pulse Sensing

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Robert Vogel ◽  
Anoop K. Pal ◽  
Siddharth Jambhrunkar ◽  
Pragnesh Patel ◽  
Sachin S. Thakur ◽  
...  
Keyword(s):  
High Resolution ◽  
Zeta Potential ◽  
Single Particle ◽  
Resistive Pulse ◽  
Tunable Resistive Pulse Sensing ◽  
Resistive Pulse Sensing ◽  
Resolution Single

Nanotubes Complexed with DNA and Proteins for Resistive-Pulse Sensing

ACS Nano ◽  
2013 ◽  
Vol 7 (10) ◽  
pp. 8857-8869 ◽  
Author(s):  
Jingjie Sha ◽  
Tawfique Hasan ◽  
Silvia Milana ◽  
Cristina Bertulli ◽  
Nicholas A. W. Bell ◽  
...  
Keyword(s):  
Resistive Pulse ◽  
Resistive Pulse Sensing

scholarly journals Nonclogging Resistive Pulse Sensing with Electrohydrodynamic Cone-Jet Bridges

2011 ◽  
Vol 1 (2) ◽  
Author(s):  
Yuejun Zhao ◽  
David B. Bober ◽  
Chuan-Hua Chen
Keyword(s):  
Resistive Pulse ◽  
Resistive Pulse Sensing

scholarly journals Microscopy and tunable resistive pulse sensing characterization of the swelling of pH-responsive, polymeric expansile nanoparticles

Nanoscale ◽  
2013 ◽  
Vol 5 (8) ◽  
pp. 3496 ◽  
Author(s):  
Aaron H. Colby ◽  
Yolonda L. Colson ◽  
Mark W. Grinstaff
Keyword(s):  
Ph Responsive ◽  
Resistive Pulse ◽  
Tunable Resistive Pulse Sensing ◽  
Resistive Pulse Sensing

Resistive‐pulse Sensing of DNA with Polymeric Nanopore Sensor and Characterization of DNA Translocation

ChemNanoMat ◽  
2021 ◽  
Author(s):  
Kaan Kececi ◽  
Dila Kaya ◽  
Charles R. Martin
Keyword(s):  
Dna Translocation ◽  
Resistive Pulse ◽  
Resistive Pulse Sensing

Tunable resistive pulse sensing and nanoindentation of pH-responsive expansile nanoparticles

2017 ◽  
Vol 14 (1/2/3/4/5/6) ◽  
pp. 446
Author(s):  
Eva Weatherall ◽  
Thomas Loho ◽  
Michelle Dickinson ◽  
Aaron H. Colby ◽  
Mark W. Grinstaff ◽  
...  
Keyword(s):  
Ph Responsive ◽  
Resistive Pulse ◽  
Tunable Resistive Pulse Sensing ◽  
Resistive Pulse Sensing
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