Immunohistochemical detection of cd1a antigen in formalin-fixed and paraffin-embedded tissue sections with monoclonal antibody 010

1993 ◽  
Vol 171 (2) ◽  
pp. 99-104 ◽  
Author(s):  
Làszlò Krenàcs ◽  
Làszlò Tiszalvicz ◽  
Tibor Krenàcs ◽  
Laurence Boumsell
Keyword(s):  
Monoclonal Antibody ◽  
Immunohistochemical Detection ◽  
Tissue Sections ◽  
Formalin Fixed

Monoclonal Antibody-based Immunohistochemical Detection of Bovine Viral Diarrhea Virus in Formalin-fixed, Paraffin-embedded Tissues

1992 ◽  
Vol 29 (1) ◽  
pp. 27-32 ◽  
Author(s):  
D. M. Haines ◽  
E. G. Clark ◽  
E. J. Dubovi
Keyword(s):  
Monoclonal Antibody ◽  
Bovine Viral Diarrhea Virus ◽  
Bovine Viral Diarrhea ◽  
Immunohistochemical Detection ◽  
Fixed Tissue ◽  
Diarrhea Virus ◽  
Viral Diarrhea ◽  
Mucosal Disease ◽  
Viral Diarrhea Virus ◽  
Formalin Fixed

Thirty-two monoclonal antibodies directed against epitopes on bovine viral diarrhea virus proteins and glycoproteins were tested for immunohistochemical reactivity with bovine viral diarrhea virus in formalin-fixed and paraffin-embedded tissues from 45 cases of bovine viral diarrhea virus-associated mucosal disease. Only one antibody, designated 15C5, which reacts with the 48-kD glycoprotein of bovine viral diarrhea virus, detected an epitope preserved in these specimens. Monoclonal antibody 15C5 and a polyclonal antibody to bovine viral diarrhea virus successfully detected bovine viral diarrhea viral antigens in 44/45 cases of mucosal disease and did not react with formalin-fixed tissues from 30 uninfected cattle. Monoclonal antibody-based immunohistochemical detection of bovine viral diarrhea virus in routinely fixed tissue specimens has advantages over other currently available techniques in terms of the convenience of specimen submission, the relative ease of method standardization, and the rapidity of the test, and by enabling identification of the virus in association with specific tissues, cell types, and histologic lesions.

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