Selvaraj Kunjiappan
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Theivendren Panneerselvam
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Balasubramanian Somasundaram
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Murugesan Sankaranarayanan
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Pavadai Parasuraman
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...
Purpose: To investigate N-succinyl chitosan nanoparticles (NSC NPs) encapsulation with Dunaliella
bardawil (D. bardawil) biomass for high utilization enhanced effectiveness and least side effects for anticancer
activity.
Methods:
The potential bioactive compounds from D. bardawil biomass were encapsulated NSC NPs by
ionotropic gelation method and to characterize its molecular shape, particle size, stability and polydispersity
index using FTIR, XRD, SEM, TEM and Zetasize Nano analyzer. Signaling pathway analysis, molecular
docking study and in vitro anticancer screening were performed on chosen H-Ras P21, 721P and liver cancer cell
lines (HepG2), respectively.
Results:
The D. bardawil biomass majorly contains 6 bioactive compounds such as β-carotene, lutein,
zeaxanthin, phytoene, canthaxanthin, and phytofluene were identified by LC-MS. The D. bardawil biomass
encapsulated NSC NPs showed an average particle size of 80±5.6 nm in spherical shape, crystalline nature, zeta
potential of -32±2.7 mV and polydispersity index of 0.51±0.02. Interestingly, the identified target using graph
theoretical signaling pathway analysis and molecular docking study showed strong interaction of NSC NPs in
binding pockets of H-Ras P21 protooncogene. At 50μg/mL, NPs displayed 95.60% cytotoxicity in HepG2 cell
line. The apoptotic cell cycle analysis showed cell death for 24 h and 48 h representing 13.13% and 47.04%,
respectively.
Conclusion:
The highly cross-linked, biocompatible, biodegradable, nontoxic NSC NPs promising carrier for
delivery of bioactive molecules present in the D. bardawil biomass was found to be actively involved in
deregulation of cellular growth in targeted cancer cells. Thus active NPs serve as a novel nanodrug to enhance
the controlled; site specific drug delivery in the management of cancer.