A new l‐ arginine oxidase engineered from l‐ glutamate oxidase

2021 ◽  
Author(s):  
Yoshika Yano ◽  
Shinsaku Matsuo ◽  
Nanako Ito ◽  
Takashi Tamura ◽  
Hitoshi Kusakabe ◽  
...  
Keyword(s):  
Glutamate Oxidase

THE ROLE OF POLYETHYLENIMINE IN ENHANCING PERFORMANCE OF GLUTAMATE BIOSENSORS

2018 ◽  
Vol 8 (3) ◽  
pp. 36-41
Author(s):  
Diep Do Thi Hong ◽  
Duong Le Phuoc ◽  
Hoai Nguyen Thi ◽  
Serra Pier Andrea ◽  
Rocchitta Gaia
Keyword(s):  
First Generation ◽  
Good Reproducibility ◽  
Complex Matrices ◽  
Long Term Stability ◽  
In Vitro Characterization ◽  
Glutamate Oxidase

Background: The first biosensor was constructed more than fifty years ago. It was composed of the biorecognition element and transducer. The first-generation enzyme biosensors play important role in monitoring neurotransmitter and determine small quantities of substances in complex matrices of the samples Glutamate is important biochemicals involved in energetic metabolism and neurotransmission. Therefore, biosensors requires the development a new approach exhibiting high sensibility, good reproducibility and longterm stability. The first-generation enzyme biosensors play important role in monitoring neurotransmitter and determine small quantities of substances in complex matrices of the samples. The aims of this work: To find out which concentration of polyethylenimine (PEI) exhibiting the most high sensibility, good reproducibility and long-term stability. Methods: We designed and developed glutamate biosensor using different concentration of PEI ranging from 0% to 5% at Day 1 and Day 8. Results: After Glutamate biosensors in-vitro characterization, several PEI concentrations, ranging from 0.5% to 1% seem to be the best in terms of VMAX, the KM; while PEI content ranging from 0.5% to 1% resulted stable, PEI 1% displayed an excellent stability. Conclusions: In the result, PEI 1% perfomed high sensibility, good stability and blocking interference. Furthermore, we expect to develop and characterize an implantable biosensor capable of detecting glutamate, glucose in vivo. Key words: Glutamate biosensors, PEi (Polyethylenimine) enhances glutamate oxidase, glutamate oxidase biosensors

scholarly journals An Electrochemical Evaluation of Novel Ferrocene Derivatives for Glutamate and Liver Biomarker Biosensing

Biosensors ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 254
Author(s):  
Geok Hong Soon ◽  
Mary Deasy ◽  
Eithne Dempsey
Keyword(s):  
Film Studies ◽  
Redox Potentials ◽  
Signal Loss ◽  
Electronic Effects ◽  
Ferrocene Derivatives ◽  
Electroactive Films ◽  
Glutamate Oxidase ◽  
Electronic Effects Of Substituents ◽  
Glutamyl Transpeptidase

Here, we present an evaluation of two new monosubstituted ferrocene (Fc) derivatives, 3-(1H-pyrrol-1-yl)propanamidoferrocene and 1-hydroxy-2-[2-(thiophen-3-yl)-ethylamino]ethylferrocene, as glutamate oxidase mediators, together with their preparation and characterisation. Taking into consideration the influence of the electronic effects of substituents on the redox potentials of the Fc species, two candidates with pyrrole or thiophene moieties were proposed for investigation. Film studies involved potential sweeping in the presence of pyrrole or 3,4-ethylenedioxythiophene monomers resulting in stable electroactive films with % signal loss upon cycling ranging from 1 to 7.82% and surface coverage (Γ) 0.47–1.15 × 10−9 mol/cm2 for films formed under optimal conditions. Construction of a glutamate oxidase modified electrode resulted in second-generation biosensing with the aid of both cyclic voltammetry and hydrodynamic amperometry, resulting in glutamate sensitivity of 0.86–1.28 μA/mM and Km (app) values over the range 3.67–5.01 mM. A follow-up enzyme assay for liver biomarker γ-glutamyl transpeptidase realised unmediated and mediated measurement establishing reaction and incubation time investigations and a realising response over <100 U/L γ-glutamyl transpeptidase with a sensitivity of 5 nA/UL−1.

Quantitation of Monosodium Glutamate Using Immobilized Glutamate Oxidase/Peroxidase and Flow Injection Analysis

2000 ◽  
Vol 33 (12) ◽  
pp. 2407-2423 ◽  
Author(s):  
U. Mankasingh ◽  
D. Narinesingh ◽  
T.T. Ngo
Keyword(s):  
Flow Injection ◽  
Flow Injection Analysis ◽  
Monosodium Glutamate ◽  
Glutamate Oxidase

scholarly journals Isolation, purification, and characterization of L-glutamate oxidase from Streptomyces sp. 18G

2004 ◽  
Vol 7 (3) ◽  
Author(s):  
Suchat Udomsopagit ◽  
Supawadee Wachiratianchai ◽  
Amaret Bhumiratana
Keyword(s):  
Purification And Characterization ◽  
Streptomyces Sp ◽  
Glutamate Oxidase

Rapid and Simple Assay of Glutaminase and Leucine Amino-peptidase Activities of Shoyu Koji Usingl-Glutamate Oxidase

1984 ◽  
Vol 48 (5) ◽  
pp. 1357-1358
Author(s):  
Hitoshi Kusakabe ◽  
Yuichiro Midorikawa ◽  
Tetsuro Fujishima
Keyword(s):  
Glutamate Oxidase ◽  
Leucine Amino Peptidase

Promoter engineering of cascade biocatalysis for α-ketoglutaric acid production by coexpressing l-glutamate oxidase and catalase

2018 ◽  
Vol 102 (11) ◽  
pp. 4755-4764 ◽  
Author(s):  
Jing Wu ◽  
Xiangchen Fan ◽  
Jia Liu ◽  
Qiuling Luo ◽  
Jisi Xu ◽  
...  
Keyword(s):  
Acid Production ◽  
Promoter Engineering ◽  
Glutamate Oxidase ◽  
Cascade Biocatalysis

Development of glutaric acid production consortium system with α-ketoglutaric acid regeneration by glutamate oxidase in Escherichia coli

2020 ◽  
Vol 133 ◽  
pp. 109446 ◽  
Author(s):  
Soo-Yeon Yang ◽  
Tae-Rim Choi ◽  
Hye-Rim Jung ◽  
Ye-Lim Park ◽  
Yeong-Hoon Han ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Acid Production ◽  
Glutaric Acid ◽  
Glutamate Oxidase

Accurate glutamate monitoring in foodstuffs by a sensitive and interference-free glutamate oxidase based disposable amperometric biosensor

2020 ◽  
Vol 1115 ◽  
pp. 16-22 ◽  
Author(s):  
Annalisa Mentana ◽  
Donatella Nardiello ◽  
Carmen Palermo ◽  
Diego Centonze
Keyword(s):  
Amperometric Biosensor ◽  
Glutamate Oxidase

Rapid measurement of transaminase activities using an amperometric l-glutamate-sensing electrode based on a glutamate oxidase–polyion complex-bilayer membrane

1998 ◽  
Vol 52 (1-2) ◽  
pp. 23-29 ◽  
Author(s):  
Fumio Mizutani ◽  
Yukari Sato ◽  
Takahiro Sawaguchi ◽  
Soichi Yabuki ◽  
Seiichiro Iijima
Keyword(s):  
Bilayer Membrane ◽  
Polyion Complex ◽  
Rapid Measurement ◽  
Glutamate Oxidase
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