scholarly journals Probing the role of tryptophan residues in a cellulose-binding domain by chemical modification

1996 ◽  
Vol 5 (11) ◽  
pp. 2311-2318 ◽  
Author(s):  
Mark R. Bray ◽  
Neil R. Gilkes ◽  
Douglas G. Kilburn ◽  
R. Antony J. Warren ◽  
Lawrence P. Mcintosh ◽  
...  
Keyword(s):  
Chemical Modification ◽  
Binding Domain ◽  
Cellulose Binding Domain ◽  
Tryptophan Residues ◽  
Cellulose Binding

scholarly journals The role of conserved tryptophan residues in the interaction of a bacterial cellulose binding domain with its ligand

1993 ◽  
Vol 106 (1) ◽  
pp. 77-83 ◽  
Author(s):  
Debbie M. Poole ◽  
Geoffrey P. Hazlewood ◽  
Neville S. Huskisson ◽  
Richard Virden ◽  
Harry J. Gilbert
Keyword(s):  
Bacterial Cellulose ◽  
Binding Domain ◽  
Cellulose Binding Domain ◽  
Tryptophan Residues ◽  
Cellulose Binding

The cellulose-binding domain of endoglucanase A (CenA) from Cellulomonas fimi: evidence for the involvement of tryptophan residues in binding

1994 ◽  
Vol 11 (4) ◽  
pp. 747-755 ◽  
Author(s):  
Neena Din ◽  
Ian J. Forsythe ◽  
Leslie D. Burtnick ◽  
Neil R. Gilkes ◽  
Robert C. Miller ◽  
...  
Keyword(s):  
Binding Domain ◽  
Cellulose Binding Domain ◽  
Cellulomonas Fimi ◽  
Tryptophan Residues ◽  
Cellulose Binding

Role of Cellulose-Binding Domain of Exocellulase I from White Rot Basidiomycete Irpex lacteus.

2001 ◽  
Vol 91 (4) ◽  
pp. 359-362 ◽  
Author(s):  
NAOKO HAMADA ◽  
RITSUKO KODAIRA ◽  
MASAHIRO NOGAWA ◽  
KAZUNORI SHINJI ◽  
RIE ITO ◽  
...  
Keyword(s):  
Binding Domain ◽  
White Rot ◽  
Cellulose Binding Domain ◽  
Irpex Lacteus ◽  
Cellulose Binding

Role of cellulose-binding domain of exocellulase I from White rot basidiomycete Irpex lacteus

2001 ◽  
Vol 91 (4) ◽  
pp. 359-362 ◽  
Author(s):  
Naoko Hamada ◽  
Ritsuko Kodaira ◽  
Masahiro Nogawa ◽  
Kazunori Shinji ◽  
Rie Ito ◽  
...  
Keyword(s):  
Binding Domain ◽  
White Rot ◽  
Cellulose Binding Domain ◽  
Irpex Lacteus ◽  
Cellulose Binding

scholarly journals Cellulose as an inert matrix for presenting cytokines to target cells: production and properties of a stem cell factor–cellulose-binding domain fusion protein

1999 ◽  
Vol 339 (2) ◽  
pp. 429-434
Author(s):  
J. Greg DOHENY ◽  
Eric J. JERVIS ◽  
M. Marta GUARNA ◽  
R. Keith HUMPHRIES ◽  
R. Antony J. WARREN ◽  
...  
Keyword(s):  
Stem Cell ◽  
Fusion Protein ◽  
Stem Cell Factor ◽  
Direct Analysis ◽  
Binding Domain ◽  
Target Cells ◽  
Cellulose Binding Domain ◽  
Binding Characteristics ◽  
Cellulose Binding ◽  
Cellulose Surface

A chimaera of stem cell factor (SCF) and a cellulose-binding domain from the xylanase Cex (CBDCex) effectively immobilizes SCF on a cellulose surface. The fusion protein retains both the cytokine properties of SCF and the cellulose-binding characteristics of CBDCex. When adsorbed on cellulose, SCF–CBDCex is up to 7-fold more potent than soluble SCF–CBDCex and than native SCF at stimulating the proliferation of factor-dependent cell lines. When cells are incubated with cellulose-bound SCF–CBDCex, activated receptors and SCF–CBDCex co-localize on the cellulose matrix. The strong binding of SCF–CBDCex to the cellulose surface permits the effective and localized stimulation of target cells; this is potentially significant for long-term perfusion culturing of factor-dependent cells. It also permits the direct analysis of the effects of surface-bound cytokines on target cells.

scholarly journals Fusion Endoglucanase Cel12B from Thermotoga maritima with Cellulose Binding Domain

BioResources ◽  
2018 ◽  
Vol 13 (2) ◽  
Author(s):  
Hao Shi ◽  
Yanling Chen ◽  
Wenjian Peng ◽  
Pixiang Wang ◽  
Yuping Zhao ◽  
...  
Keyword(s):  
Thermotoga Maritima ◽  
Binding Domain ◽  
Cellulose Binding Domain ◽  
Cellulose Binding
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