Kinetic folding and unfolding of staphylococcal nuclease and its six mutants studied by stopped-flow circular dichroism

1995 ◽  
Vol 23 (2) ◽  
pp. 163-176 ◽  
Author(s):  
Nikolay N. Kalnin ◽  
Kunihiro Kuwajima
Keyword(s):  
Circular Dichroism ◽  
Staphylococcal Nuclease ◽  
Stopped Flow ◽  
Circular Dichroïsm ◽  
Kinetic Folding

scholarly journals Least activation path for protein folding: investigation of staphylococcal nuclease folding by stopped-flow circular dichroism.

1996 ◽  
Vol 93 (6) ◽  
pp. 2539-2544 ◽  
Author(s):  
Z. D. Su ◽  
M. T. Arooz ◽  
H. M. Chen ◽  
C. J. Gross ◽  
T. Y. Tsong
Keyword(s):  
Protein Folding ◽  
Circular Dichroism ◽  
Staphylococcal Nuclease ◽  
Stopped Flow ◽  
Circular Dichroïsm

Characterization of Kinetic Folding Intermediates of Recombinant Canine Milk Lysozyme by Stopped-Flow Circular Dichroism†

Biochemistry ◽  
2005 ◽  
Vol 44 (17) ◽  
pp. 6685-6692 ◽  
Author(s):  
Masaharu Nakao ◽  
Kosuke Maki ◽  
Munehito Arai ◽  
Takumi Koshiba ◽  
Katsutoshi Nitta ◽  
...  
Keyword(s):  
Circular Dichroism ◽  
Stopped Flow ◽  
Folding Intermediates ◽  
Circular Dichroïsm ◽  
Kinetic Folding

Stopped-flow circular dichroism: A new fast-kinetic system

Biopolymers ◽  
1974 ◽  
Vol 13 (2) ◽  
pp. 401-405 ◽  
Author(s):  
P. M. Bayley ◽  
M. Anson
Keyword(s):  
Circular Dichroism ◽  
Stopped Flow ◽  
Kinetic System ◽  
Fast Kinetic ◽  
Circular Dichroïsm

Stopped-flow circular dichroism

2000 ◽  
Author(s):  
Alison Rodger ◽  
Michael J. Carey
Keyword(s):  
Circular Dichroism ◽  
Detection Methods ◽  
Side Reactions ◽  
Stopped Flow ◽  
Baseline Drift ◽  
Mixing Ratios ◽  
Flow Mixing ◽  
Ideal Tool ◽  
Kinetics Of ◽  
Circular Dichroïsm

As is apparent from previous chapters (Chapters 6, 8 and 9), understanding the kinetics of chemical and biological processes is extremely important. Questions we often consider, explicitly or implicitly, include: Has something happened ‘instantaneously’ or will it take 20 years? Does changing the conditions or available reagents affect either the end product or the rate of a process? What intermediates are produced during a reaction? Can we characterize any intermediates? Do we need to remove them to prevent side reactions? If some or all of the reactants or products are chiral, then circular dichroism (CD) detection may be the ideal tool for following the kinetics of a reaction, and if the half-life of the reaction is of the order of milliseconds to seconds or even minutes then stopped-flow mixing of the reagents will almost certainly be the appropriate choice of sample handling method. For reactions with half-lives of a few minutes to tens of minutes the reagents can be mixed by hand in a normal cuvette and the signal monitored at an appropriate wavelength. CD is not well suited to kinetics on timescales of hours due to the baseline drift that does occur (see Chapter 4, Section 2.5). Some CD spectropolarimeters have the useful facility of being able to perform a wavelength scan at pre-set intervals as well as monitoring continuously (except during the wavelength scan) at a chosen wavelength, thus facilitating the characterization of any intermediates. In this chapter we shall highlight some of the considerations of the stopped-flow technique that are particularly relevant to CD experiments. Particular problems may be encountered when performing CD (as opposed to other detection methods) stopped-flow experiments. The measured signals are very small (typical CD intensities are 0.1% or less of the absorbance signal), and the noise level observed is particularly sensitive to any inhomogeneities or turbulence in the samples. Also, as one of the main applications of stopped-flow CD is in the study of protein folding and unfolding, samples are often very viscous and/or corrosive, have significant absorbances due to buffers etc., and the experiments often require wide and variable mixing ratios.

Structure of a Compact Peptide from Staphylococcal Nuclease Determined by Circular Dichroism and NMR Spectroscopy

Biochemistry ◽  
1995 ◽  
Vol 34 (17) ◽  
pp. 5795-5800 ◽  
Author(s):  
Mark W. Maciejewski ◽  
Micheal H. Zehfus
Keyword(s):  
Circular Dichroism ◽  
Nmr Spectroscopy ◽  
Staphylococcal Nuclease ◽  
Circular Dichroïsm

scholarly journals Reconstitution of myoglobin from apoprotein and heme, monitored by stopped-flow absorption, fluorescence and circular dichroism

1988 ◽  
Vol 170 (3) ◽  
pp. 589-595 ◽  
Author(s):  
Yasuko KAWAMURA-KONISHI ◽  
Hiroshi KIHARA ◽  
Haruo SUZUKI
Keyword(s):  
Circular Dichroism ◽  
Stopped Flow ◽  
Circular Dichroïsm
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