Molecular characterization of the two-component histidine kinase gene fromMonilinia fructicola

Anabaena sp. PCC 7120 was mutagenized by transposon Tn5-1087b, generating a mutant whose heterocysts lack the envelope polysaccharide layer. The transposon was located between nucleotides 342 and 343 of alr0117, a 918 bp gene encoding a histidine kinase for a two-component regulatory system. Complementation of the mutant with a DNA fragment containing alr0117 and targeted inactivation of the gene confirmed that alr0117 is involved in heterocyst development. RT-PCR showed that alr0117 was constitutively expressed in the presence or absence of a combined-nitrogen source. hepA and patB, the two genes turned on during wild-type heterocyst development, were no longer activated in an alr0117-null mutant. The two-component signal transduction system involving alr0117 may control the formation of the envelope polysaccharide layer and certain late events essential to the function of heterocysts.

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Isolation and molecular characterization of the swinepox virus thymidine kinase gene

Virology ◽

10.1016/0042-6822(91)90986-l ◽

1991 ◽

Vol 183 (2) ◽

pp. 578-585 ◽

Cited By ~ 9

Author(s):

J.A. Feller ◽

R.F. Massung ◽

P.C. Turner ◽

E.P.J. Gibbs ◽

E.O. Bockamp ◽

...

Keyword(s):

Thymidine Kinase ◽

Molecular Characterization ◽

Thymidine Kinase Gene ◽

Kinase Gene ◽

Swinepox Virus

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Cloning and characterization of the histidine kinase gene Dic1 from Cochliobolus heterostrophus that confers dicarboximide resistance and osmotic adaptation

Molecular Genetics and Genomics ◽

10.1007/s00438-003-0974-4 ◽

2004 ◽

Vol 271 (2) ◽

pp. 228-236 ◽

Cited By ~ 60

Author(s):

A. Yoshimi ◽

M. Tsuda ◽

C. Tanaka

Keyword(s):

Histidine Kinase ◽

Cochliobolus Heterostrophus ◽

Kinase Gene ◽

Osmotic Adaptation ◽

Dicarboximide Resistance

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Dicarboximide resistance in field isolates of Alternaria alternata is mediated by a mutation in a two-component histidine kinase gene

Fungal Genetics and Biology ◽

10.1016/j.fgb.2003.09.002 ◽

2004 ◽

Vol 41 (1) ◽

pp. 102-108 ◽

Cited By ~ 40

Author(s):

Ian B Dry ◽

Khor H Yuan ◽

Don G Hutton

Keyword(s):

Alternaria Alternata ◽

Histidine Kinase ◽

Kinase Gene ◽

Field Isolates ◽

Two Component ◽

Dicarboximide Resistance

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Molecular Characterization of Two-Component Systems of Helicobacter pylori

Journal of Bacteriology ◽

10.1128/jb.182.8.2068-2076.2000 ◽

2000 ◽

Vol 182 (8) ◽

pp. 2068-2076 ◽

Cited By ~ 126

Author(s):

Dagmar Beier ◽

Rainer Frank

Keyword(s):

Helicobacter Pylori ◽

Histidine Kinase ◽

Response Regulator ◽

Response Regulators ◽

Reading Frame ◽

Component Systems ◽

Two Component ◽

Two Component Systems

ABSTRACT Two-component systems are frequently involved in the adaptation of bacteria to changing environmental conditions at the level of transcriptional regulation. Here we report the characterization of members of the two-component systems of the gastric pathogenHelicobacter pylori deduced from the genome sequence of strain 26695. We demonstrate that the response regulators HP166, HP1043, and HP1021 have essential functions, as disruption of the corresponding genes is lethal for the bacteria, irrespective of the fact that HP1043 and HP1021 have nonconserved substitutions in crucial amino acids of their receiver domains. An analysis of the in vitro phosphorylation properties of the two-component proteins demonstrates that HP244-HP703 and HP165-HP166 are cognate histidine kinase-response regulator pairs. Furthermore, we provide evidence that the variability of the histidine kinase HP165 caused by a poly(C) tract of variable length close to the 3′ end of open reading frame 165/164 does not interfere with the kinase activity of the transmitter domain of HP165.

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