Characterization of mutations in the two-component histidine kinase gene AbNIK1 from Alternaria brassicicola that confer high dicarboximide and phenylpyrrole resistance

2005 ◽  
Vol 47 (4) ◽  
pp. 234-243 ◽  
Author(s):  
Herv� Avenot ◽  
Philippe Simoneau ◽  
B�atrice Iacomi-Vasilescu ◽  
Nelly Bataill�-Simoneau
Keyword(s):  
Histidine Kinase ◽  
Alternaria Brassicicola ◽  
Kinase Gene ◽  
Two Component

Molecular characterization of the two-component histidine kinase gene fromMonilinia fructicola

2006 ◽  
Vol 62 (10) ◽  
pp. 991-998 ◽  
Author(s):  
Zhonghua Ma ◽  
Yong Luo ◽  
Themis Michailides
Keyword(s):  
Molecular Characterization ◽  
Histidine Kinase ◽  
Kinase Gene ◽  
Two Component

Characterization of mutations in the two-component histidine kinase gene that confer fludioxonil resistance and osmotic sensitivity in theos-1 mutants ofNeurospora crassa

2001 ◽  
Vol 57 (5) ◽  
pp. 437-442 ◽  
Author(s):  
Noriyuki Ochiai ◽  
Makoto Fujimura ◽  
Takayuki Motoyama ◽  
Akihiko Ichiishi ◽  
Ron Usami ◽  
...  
Keyword(s):  
Histidine Kinase ◽  
Kinase Gene ◽  
Osmotic Sensitivity ◽  
Ofneurospora Crassa ◽  
Two Component

alr0117, a two-component histidine kinase gene, is involved in heterocyst development in Anabaena sp. PCC 7120

Microbiology ◽  
2004 ◽  
Vol 150 (2) ◽  
pp. 447-453 ◽  
Author(s):  
Degang Ning ◽  
Xudong Xu
Keyword(s):  
Histidine Kinase ◽  
Regulatory System ◽  
Gene Encoding ◽  
Kinase Gene ◽  
Two Component ◽  
Two Component Signal Transduction ◽  
Anabaena Sp ◽  
Pcc 7120 ◽  
Polysaccharide Layer ◽  
Heterocyst Development

Anabaena sp. PCC 7120 was mutagenized by transposon Tn5-1087b, generating a mutant whose heterocysts lack the envelope polysaccharide layer. The transposon was located between nucleotides 342 and 343 of alr0117, a 918 bp gene encoding a histidine kinase for a two-component regulatory system. Complementation of the mutant with a DNA fragment containing alr0117 and targeted inactivation of the gene confirmed that alr0117 is involved in heterocyst development. RT-PCR showed that alr0117 was constitutively expressed in the presence or absence of a combined-nitrogen source. hepA and patB, the two genes turned on during wild-type heterocyst development, were no longer activated in an alr0117-null mutant. The two-component signal transduction system involving alr0117 may control the formation of the envelope polysaccharide layer and certain late events essential to the function of heterocysts.

scholarly journals Molecular Characterization of Two-Component Systems of Helicobacter pylori

2000 ◽  
Vol 182 (8) ◽  
pp. 2068-2076 ◽  
Author(s):  
Dagmar Beier ◽  
Rainer Frank
Keyword(s):  
Helicobacter Pylori ◽  
Histidine Kinase ◽  
Response Regulator ◽  
Response Regulators ◽  
Reading Frame ◽  
Component Systems ◽  
Two Component ◽  
Two Component Systems

ABSTRACT Two-component systems are frequently involved in the adaptation of bacteria to changing environmental conditions at the level of transcriptional regulation. Here we report the characterization of members of the two-component systems of the gastric pathogenHelicobacter pylori deduced from the genome sequence of strain 26695. We demonstrate that the response regulators HP166, HP1043, and HP1021 have essential functions, as disruption of the corresponding genes is lethal for the bacteria, irrespective of the fact that HP1043 and HP1021 have nonconserved substitutions in crucial amino acids of their receiver domains. An analysis of the in vitro phosphorylation properties of the two-component proteins demonstrates that HP244-HP703 and HP165-HP166 are cognate histidine kinase-response regulator pairs. Furthermore, we provide evidence that the variability of the histidine kinase HP165 caused by a poly(C) tract of variable length close to the 3′ end of open reading frame 165/164 does not interfere with the kinase activity of the transmitter domain of HP165.

Isolation and transcript analysis of two-component histidine kinase gene Le.nik1 in Shiitake mushroom, Lentinula edodes

2008 ◽  
Vol 112 (1) ◽  
pp. 108-116 ◽  
Author(s):  
Carol Y.Y. Szeto ◽  
Queenie W.L. Wong ◽  
Grace S. Leung ◽  
H.S. Kwan
Keyword(s):  
Histidine Kinase ◽  
Lentinula Edodes ◽  
Transcript Analysis ◽  
Shiitake Mushroom ◽  
Kinase Gene ◽  
Two Component

scholarly journals Effect of new alleles of the histidine kinase gene ciaH on the activity of the response regulator CiaR in Streptococcus pneumoniae R6

Microbiology ◽  
2011 ◽  
Vol 157 (11) ◽  
pp. 3104-3112 ◽  
Author(s):  
Miriam Müller ◽  
Patrick Marx ◽  
Regine Hakenbeck ◽  
Reinhold Brückner
Keyword(s):  
Streptococcus Pneumoniae ◽  
Histidine Kinase ◽  
Response Regulator ◽  
Regulatory System ◽  
Competence Development ◽  
Kinase Gene ◽  
Lactam Antibiotic ◽  
Spontaneous Mutants ◽  
New Alleles

The two-component regulatory system CiaRH of Streptococcus pneumoniae affects β-lactam susceptibility, autolysis, bacteriocin production, competence development, host colonization and virulence. The system was discovered in a screen for S. pneumoniae R6 mutants resistant to the β-lactam antibiotic cefotaxime. A mutation in the histidine kinase gene ciaH led to this phenotype by enhancing CiaR-mediated gene expression. Additional mutations in ciaH have been described in other spontaneous β-lactam-resistant mutants of S. pneumoniae R6, but their influence on CiaR-mediated gene regulation has not been determined. Likewise, altered ciaH alleles are present in clinical S. pneumoniae isolates, none of which had been characterized. These novel ciaH variants were introduced into S. pneumoniae R6 to measure their ability to activate CiaR-dependent regulation. The ciaH alleles from spontaneous mutants obtained in the laboratory increased the activity of CiaR-dependent promoters between four- and 26-fold, while variants from clinical strains were less effective, with a threefold activation at most. Accordingly, phenotypes associated with a hyperactive CiaRH system, β-lactam resistance, and prevention of competence development, were far more pronounced in the laboratory mutants. Amino acid changes affecting CiaH function were positioned throughout the protein. Five of the most activating changes are located close to the conserved histidine and one in the extracytoplasmic sensor domain. The characterization of new alleles of ciaH expands the spectrum of CiaH variants, which may help to elucidate signal transduction of this important regulatory system. Our study also demonstrates that ciaH alleles overstimulating CiaR regulon expression are present in clinical isolates of S. pneumoniae.

scholarly journals The response regulator PhoP4 is required for late developmental events in Myxococcus xanthus

Microbiology ◽  
2006 ◽  
Vol 152 (6) ◽  
pp. 1609-1620 ◽  
Author(s):  
Vinh D. Pham ◽  
Conrad W. Shebelut ◽  
Ivy R. Jose ◽  
David A. Hodgson ◽  
David E. Whitworth ◽  
...  
Keyword(s):  
Histidine Kinase ◽  
Response Regulator ◽  
Myxococcus Xanthus ◽  
Kinase Gene ◽  
Spore Viability ◽  
Phenotypic Differences ◽  
Component Systems ◽  
Two Component Systems ◽  
Identification And Characterization

Phosphate regulation is complex in the developmental prokaryote Myxococcus xanthus, and requires at least four two-component systems (TCSs). Here, the identification and characterization of a member of one TCS, designated PhoP4, is reported. phoP4 insertion and in-frame deletion strains caused spore viability to be decreased by nearly two orders of magnitude, and reduced all three development-specific phosphatase activities by 80–90 % under phosphate-limiting conditions. Microarray and quantitative PCR analyses demonstrated that PhoP4 is also required for appropriate expression of the predicted pstSCAB–phoU operon of inorganic phosphate assimilation genes. Unlike the case for the other three M. xanthus Pho TCSs, the chromosomal region around phoP4 does not contain a partner histidine kinase gene. Yeast two-hybrid analyses reveal that PhoP4 interacts reciprocally with PhoR2, the histidine kinase of the Pho2 TCS; however, the existence of certain phenotypic differences between phoP4 and phoR2 mutants suggests that PhoP4 interacts with another, as-yet unidentified, histidine kinase.

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