Domain organization and molecular characterization of 13 two-component systems identified by genome sequencing of Streptococcus pneumoniae

Gene ◽  
1999 ◽  
Vol 237 (1) ◽  
pp. 223-234 ◽  
Author(s):  
Roland Lange ◽  
Christian Wagner ◽  
Antoine de Saizieu ◽  
Nicholas Flint ◽  
Juliette Molnos ◽  
...  
Keyword(s):  
Streptococcus Pneumoniae ◽  
Molecular Characterization ◽  
Genome Sequencing ◽  
Domain Organization ◽  
Component Systems ◽  
Two Component ◽  
Two Component Systems

scholarly journals Molecular Characterization of Two-Component Systems of Helicobacter pylori

2000 ◽  
Vol 182 (8) ◽  
pp. 2068-2076 ◽  
Author(s):  
Dagmar Beier ◽  
Rainer Frank
Keyword(s):  
Helicobacter Pylori ◽  
Histidine Kinase ◽  
Response Regulator ◽  
Response Regulators ◽  
Reading Frame ◽  
Component Systems ◽  
Two Component ◽  
Two Component Systems

ABSTRACT Two-component systems are frequently involved in the adaptation of bacteria to changing environmental conditions at the level of transcriptional regulation. Here we report the characterization of members of the two-component systems of the gastric pathogenHelicobacter pylori deduced from the genome sequence of strain 26695. We demonstrate that the response regulators HP166, HP1043, and HP1021 have essential functions, as disruption of the corresponding genes is lethal for the bacteria, irrespective of the fact that HP1043 and HP1021 have nonconserved substitutions in crucial amino acids of their receiver domains. An analysis of the in vitro phosphorylation properties of the two-component proteins demonstrates that HP244-HP703 and HP165-HP166 are cognate histidine kinase-response regulator pairs. Furthermore, we provide evidence that the variability of the histidine kinase HP165 caused by a poly(C) tract of variable length close to the 3′ end of open reading frame 165/164 does not interfere with the kinase activity of the transmitter domain of HP165.

scholarly journals Vancomycin Stress Response in a Sensitive and a Tolerant Strain of Streptococcus pneumoniae

2005 ◽  
Vol 187 (23) ◽  
pp. 8205-8210 ◽  
Author(s):  
Wolfgang Haas ◽  
Deepak Kaushal ◽  
Jack Sublett ◽  
Caroline Obert ◽  
Elaine I. Tuomanen
Keyword(s):  
Streptococcus Pneumoniae ◽  
Stress Response ◽  
Antibiotic Treatment ◽  
Tolerant Strain ◽  
Component Systems ◽  
Two Component ◽  
Genes Encoding ◽  
Two Component Systems

ABSTRACT The vancomycin stress response was studied in Streptococcus pneumoniae strains T4 (TIGR4) and Tupelo. Vancomycin affected the expression of 175 genes, including genes encoding transport functions and enzymes involved in aminosugar metabolism. The two-component systems TCS03, TCS11, and CiaRH also responded to antibiotic treatment. We hypothesize that the three regulons are an important part of the bacterium's response to vancomycin stress.

Sol–gel synthesis and characterization of two-component systems based on MgO

2017 ◽  
Vol 82 (2) ◽  
pp. 611-619 ◽  
Author(s):  
Aleksey A. Vedyagin ◽  
Ilya V. Mishakov ◽  
Timofey M. Karnaukhov ◽  
Elena F. Krivoshapkina ◽  
Ekaterina V. Ilyina ◽  
...  
Keyword(s):  
Sol Gel ◽  
Synthesis And Characterization ◽  
Component Systems ◽  
Two Component ◽  
Two Component Systems ◽  
Sol Gel Synthesis

A modified repair strategy for two-component systems with revealed and unrevealed faults

1987 ◽  
Vol 24 (01) ◽  
pp. 178-185
Author(s):  
Norman L. Johnson ◽  
Samuel Kotz
Keyword(s):  
Repair System ◽  
Two Component System ◽  
Initial State ◽  
Repair Strategy ◽  
Component Systems ◽  
Two Component ◽  
Two Component Systems ◽  
The Times ◽  
The One

Some consequences of a modified repair system for Phillips' (1981a, b) model for a two-component system are discussed. In the original model, both components are repaired whenever a revealed fault occurs; in the modified model only faulty components are repaired. Specifically (i) the distribution of time from the initial state up to discovery of an unrevealed fault, (ii) the expected proportion of time during which there exists an unrepaired fault, and (iii) the distribution of number of revealed faults up to and including the one which leads to a discovery of an unrevealed fault, are obtained. The theory is illustrated by examples, based on specific distributions for the times between repairs and occurrences of the two types of faults. A characterization of the exponential distribution is indicated.

A modified repair strategy for two-component systems with revealed and unrevealed faults

1987 ◽  
Vol 24 (1) ◽  
pp. 178-185 ◽  
Author(s):  
Norman L. Johnson ◽  
Samuel Kotz
Keyword(s):  
Repair System ◽  
Two Component System ◽  
Initial State ◽  
Repair Strategy ◽  
Component Systems ◽  
Two Component ◽  
Two Component Systems ◽  
The Times ◽  
The One

Some consequences of a modified repair system for Phillips' (1981a, b) model for a two-component system are discussed. In the original model, both components are repaired whenever a revealed fault occurs; in the modified model only faulty components are repaired. Specifically (i) the distribution of time from the initial state up to discovery of an unrevealed fault, (ii) the expected proportion of time during which there exists an unrepaired fault, and (iii) the distribution of number of revealed faults up to and including the one which leads to a discovery of an unrevealed fault, are obtained. The theory is illustrated by examples, based on specific distributions for the times between repairs and occurrences of the two types of faults. A characterization of the exponential distribution is indicated.

scholarly journals In silico characterization of three two-component systems of Ehrlichia canis and evaluation of a natural plant-derived inhibitor

2012 ◽  
Vol 11 (4) ◽  
pp. 3576-3584
Author(s):  
E.V. Santos ◽  
G. Silva ◽  
G.P. Cardozo ◽  
T.A. Bitencourt ◽  
S.C. França ◽  
...  
Keyword(s):  
In Silico ◽  
Ehrlichia Canis ◽  
Natural Plant ◽  
Component Systems ◽  
Two Component ◽  
Two Component Systems ◽  
In Silico Characterization

scholarly journals Rapid identification, capsular typing and molecular characterization of Streptococcus pneumoniae by using whole genome nanopore sequencing

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
S. Garcia-Garcia ◽  
A. Perez-Arguello ◽  
D. Henares ◽  
N. Timoneda ◽  
C. Muñoz-Almagro
Keyword(s):  
Streptococcus Pneumoniae ◽  
Whole Genome Sequencing ◽  
Real Time ◽  
Molecular Characterization ◽  
Genome Sequencing ◽  
Rapid Identification ◽  
Whole Genome ◽  
Genomic Epidemiology ◽  
Capsular Typing

Abstract Background Whole genome sequencing has emerged as a useful tool for identification and molecular characterization of pathogens. MinION (Oxford Nanopore) is a real-time third generation sequencer whose portability, affordability and speed in data production make of it an attractive device for whole genome sequencing. The objective of this study is to evaluate MinION sequencer for pathogen identification and molecular characterization of Streptococcus pneumoniae isolated at a children’s Hospital. Whole genome sequencing of 32 Streptococcus pneumoniae invasive isolates, previously characterized by standard methods (Quellung reaction, Multiplex PCR and Sanger-MLST), were performed. DNA was extracted using ZymoBIOMICS DNA Microprep kit. Quantification and purity of DNA was assessed by Qubit and Nanodrop, respectively. Library preparation was performed using the Rapid Barcoding Kit. Real-time workflow EPI2ME platform “What’s it in my pot” was used for species identification. Fast5 sequences were converted into FASTQ by Albacore software. Reads were assembled using CANU software. PathogenWatch, genomic epidemiology and pubmlst online tools were used for capsular typing and/or whole genome-MLST profile. Results Rapid identification of Streptococcus pneumoniae was achieved by “What’s in my pot”. Capsular typing was correctly assigned with PathogenWatch in all 32 isolates at serogroup level and 24 at serotype level. Whole genome-MLST results obtained by genomic epidemiology and pubmlst were consistent with double locus variant clonal complex obtained by Sanger-MLST in 31 isolates. Conclusion MinION sequencer provides a rapid, cost-effective and promising pathway for performing WGS by a pocked-sized device for epidemiological purposes but improving its sequencing accuracy will make it more appealing to be used in clinical microbiology laboratories.

scholarly journals Identification of Multiple Substrates of the StkP Ser/Thr Protein Kinase in Streptococcus pneumoniae

2010 ◽  
Vol 192 (14) ◽  
pp. 3629-3638 ◽  
Author(s):  
Linda Nováková ◽  
Silvia Bezoušková ◽  
Petr Pompach ◽  
Petra Špidlová ◽  
Lenka Sasková ◽  
...  
Keyword(s):  
Cell Division ◽  
Streptococcus Pneumoniae ◽  
Protein Kinase ◽  
Intracellular Signaling ◽  
Regulation Of Gene Expression ◽  
Dependent Manner ◽  
Α Subunit ◽  
Component Systems ◽  
Two Component ◽  
Two Component Systems

ABSTRACT Monitoring the external environment and responding to its changes are essential for the survival of all living organisms. The transmission of extracellular signals in prokaryotes is mediated mainly by two-component systems. In addition, genomic analyses have revealed that many bacteria contain eukaryotic-type Ser/Thr protein kinases. The human pathogen Streptococcus pneumoniae encodes 13 two-component systems and has a single copy of a eukaryotic-like Ser/Thr protein kinase gene designated stkP. Previous studies demonstrated the pleiotropic role of the transmembrane protein kinase StkP in pneumococcal physiology. StkP regulates virulence, competence, and stress resistance and plays a role in the regulation of gene expression. To determine the intracellular signaling pathways controlled by StkP, we used a proteomic approach for identification of its substrates. We detected six proteins phosphorylated on threonine by StkP continuously during growth. We identified three new substrates of StkP: the Mn-dependent inorganic pyrophosphatase PpaC, the hypothetical protein spr0334, and the cell division protein DivIVA. Contrary to the results of a previous study, we did not confirm that the α-subunit of RNA polymerase is a target of StkP. We showed that StkP activation and substrate recognition depend on the presence of a peptidoglycan-binding domain comprising four extracellular penicillin-binding protein- and Ser/Thr kinase-associated domain (PASTA domain) repeats. We found that StkP is regulated in a growth-dependent manner and likely senses intracellular peptidoglycan subunits present in the cell division septa. In addition, stkP inactivation results in cell division defects. Thus, the data presented here suggest that StkP plays an important role in the regulation of cell division in pneumococcus.

Sign in / Sign up

Export Citation Format

Share Document