The effect of plant growth regulators and nutrient conditions on growth and alkaloid production in multiple shoot cultures ofRauwolfia serpentina

1990 ◽  
Vol 4 (2) ◽  
pp. 49-52 ◽  
Author(s):  
G. Roja ◽  
B. D. Benjamin ◽  
M. R. Heble ◽  
A. V. Patankar ◽  
A. T. Sipahimalani
1987 ◽  
Vol 50 (5) ◽  
pp. 872-875 ◽  
Author(s):  
P. C. Roja ◽  
A. T. Sipahimalani ◽  
M. R. Heble ◽  
M. S. Chadha

2018 ◽  
Vol 116 ◽  
pp. 231-239 ◽  
Author(s):  
Lucila Elizabeth Fragoso Monfort ◽  
Suzan Kelly Vilela Bertolucci ◽  
Andreisa Fabri Lima ◽  
Alexandre Alves de Carvalho ◽  
Aliyu Mohammed ◽  
...  

Planta Medica ◽  
2016 ◽  
Vol 81 (S 01) ◽  
pp. S1-S381
Author(s):  
K Danova ◽  
V Motyka ◽  
M Todorova ◽  
A Trendafilova ◽  
S Krumova ◽  
...  

2008 ◽  
Vol 63 (1-2) ◽  
pp. 96-100 ◽  
Author(s):  
Wanwimon Lualon ◽  
Wanchai De-Eknamkul ◽  
Hiroyuki Tanaka ◽  
Yukihiro Shoyama ◽  
Waraporn Putalun

An efficient in vitro method for multiple shoot bud induction and regeneration has been developed in Artemisia annua L. using leaf and stem explants in various concentrations and combinations of plant growth regulators to evaluate the frequency of regeneration. The sources of explants as well as plant growth regulators in the medium were found to influence the multiple shoot induction. The result shows that the stem segment cultured on Murashige and Skoog (MS) medium supplemented with 0.1 mg/l thidiazuron (TDZ) gave a perfect shoot formation (100%) and good shoot multiplication (57 shoots/explant) after 2 weeks of culture. Healthy regenerated shoots were elongated and rooted in MS medium without hormones. The artemisinin content in plants regenerated from stem explants using 0.1 mg/l TDZ was (3.36 ± 0.36) μg/mg dry weight and two-fold higher than that of in vitro grown plants of the same age [(1.73 ± 0.23) μg/mg DW]. This system exhibited a potential for a rapid propagation of shoots from the stem explant and makes it possible to develop a clonal propagation of A. annua.


Planta Medica ◽  
1991 ◽  
Vol 57 (05) ◽  
pp. 499-500 ◽  
Author(s):  
Kazumasa Hirata ◽  
Masato Horiuchi ◽  
Teru Ando ◽  
Makiko Asada ◽  
Kazuhisa Miyamoto ◽  
...  

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