Effects of plant growth regulators, different culture media and strength MS on production of volatile fraction composition in shoot cultures of Ocimum basilicum

Marumi kumquat (Fortunella Japonica) is culture for its valuable nutritional value and medicinal importance in many regions of the world. The current study aimed to evaluate the effect of two types of media enriched with different concentrations of fructose and different plant growth regulators and different fructose concentration on in vitro propagation of Fortunella Japonica. The findings showed that the most effective treatment for explant surface sterilization was by using 0.1% HgCl2 for ten minutes which give best results for production contamination-free explants at the initiation cultures. At multiplication stage, WPM medium gave better results at all tested BA levels as compared with MS medium. No significant differences were showed by using BA alone or in combination with GA3 in the measured parameters. It has been observed that WPM medium supplemented with 0.5mgl-1 BA with the presence of 30mgl-1 fructose was able to give the highest shoot length (1.56cm) with maximum shoots number/explant 9.0 and highest leaves number/explant (21.0). The proliferated shoots were exposed to full strength MS medium salts supplemented with 2mgl-1 NAA which showed the highest ratio of rooting. In vitro rooted plantlets were gradually acclimatized and transferred to open air conditions, which recorded a high survive rate reached to 92%

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THE ROLL OF SOME PLANT GROWTH REGULATORS ON SHOOTS MULTIPLICATION OF STEVIA PLANTS IN VITRO

IRAQI JOURNAL OF AGRICULTURAL SCIENCES ◽

10.36103/ijas.v48i5.322 ◽

2017 ◽

Vol 48 (5) ◽

Author(s):

Al-Obaidy & Khierallah

Keyword(s):

Plant Growth ◽

Plant Growth Regulators ◽

Growth Regulators ◽

Culture Media ◽

Stevia Rebaudiana ◽

Shoot Multiplication ◽

Dry Weight ◽

Fresh Weight ◽

Number Of Shoots

This research was conducted to study the effect of some plant growth regulators on in vitro shoots multiplication of stevia (Stevia rebaudiana Bertoni). The experiments included tests of various combinations of KIN with IBA or IAA in the shoot multiplication. Results indicated that KIN at 1.0 mg. L-1 plus 0.3 mg. L-1 of IBA produced the highest number of shoots (3.5 shoots) while KIN at 1.5 mg. L-1 plus IBA at 1.0 mg. L-1 produced the lowest shoot length (1.14 cm). Hormone free medium produced the highest rate of the leaves number reached 28.56 leaves. KIN and IBA interaction increased fresh and dry weight significantly. Treatment contained 2.0 mg -1 KIN plus 0.3 mg. L-1 IBA produced the highest fresh weight (1.739 g) while 0.5 mg. L-1 KIN and 0.3 mg. L-1 IBA produced the highest dry weight (0.822 g). As for the effect of interaction between the IAA and KIN it was significant in the number of shoots formed. Interaction between 1.0 mg. L-1 KIN with 0.1 mg. L-1IAA produced the highest number of shoots (3.8 shoots). Shoots length reached 8.10 cm in the media with 0.3 mg. L-1 IAA only. The highest fresh weight (1.267 g) was achieved with the interaction between 1.0 mg. L-1 KIN and 0.3 mg. L-1 IAA while 0.5 mg. L-1IAA without KIN produced the highest dry weight reached 0.138 g. Shoots multiplication was improved by incorporation of the cytokinin TDZ in culture media. Shoots number, fresh and dry weights were increased significantly by adding 0.05 mg. L-1 of TDZ at present of 0.3 mg. L-1 of IBA giving 6.6 shoots, 0.974 g and 0.144 g respectively while shoots length decreased significantly as media without TDZ produced the highest shoots length reached 9.32 cm. The above results can adopt for the successful in vitro shoot multiplication of Stevia plants.

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The possibility of using Glycyrrhiza glabra L. Callus extracts as an alternative to plant growth regulators in plant tissue culture experiments

Journal of Biotechnology Research Center ◽

10.24126/jobrc.2008.2.1.30 ◽

2008 ◽

Vol 2 (1) ◽

pp. 71-80

Author(s):

Kadhim M. Ibrahim ◽

Ashwaq S. Abed ◽

E. A. Abbod ◽

Kholood W. Alsamarae ◽

Nabeel K. ALani

Keyword(s):

Plant Growth ◽

Plant Growth Regulators ◽

Growth Regulators ◽

Callus Induction ◽

Callus Tissue ◽

Culture Media ◽

Water Extract ◽

Shoot Proliferation ◽

Soya Bean ◽

Tissue Extracts

Liquorice plant is considered one of the important medicinal and economical plants. It is rich with many compounds, minerals, vitamins, and even plant hormones. This research is aimed to study the possibility of using callus tissue extracts as an alternative to plant growth regulators added to the culture media. A factorial experiment was implemented to find out the appropriate combination between 2, 4-D and BA for callus induction on Liquoricenode explants. It was found that a combination of 2 mg/l 2, 4-D with 2.5 mg/l BA is the best one for callus induction and maintenance using MS medium. Water and alcoholic extracts were prepared from callus tissue at concentrations (0, 2, 4, 6, 8 or 10) ml/l then added to culture medium as an alternative to plant growth regulators. The effect of these concentrations on growth and development of tissues and organs for some plants was studied using soya bean, potato and wheat plants for this purpose. Results showed that water extract induced shoot proliferation from potato single nodes. Both types of extracts increased soya bean callus fresh weight significantly. It was found also that water extract was more effective than alcoholic one in increasing vegetative and root parts in germinating wheat seeds.

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Effects of different plant growth regulators on in vitro callus induction in physic nut (Jatropha curcus L.)

Journal of Applied and Natural Science ◽

10.31018/jans.v7i1.559 ◽

2015 ◽

Vol 7 (1) ◽

pp. 30-37 ◽

Cited By ~ 2

Author(s):

Sunil Kumar ◽

Virendra Kumar ◽

Manoj Kumar Sharma ◽

Narendra Kumar ◽

Anil Kumar ◽

...

Keyword(s):

Plant Growth ◽

Plant Growth Regulators ◽

Growth Regulators ◽

Callus Induction ◽

Culture Media ◽

Nodal Segments ◽

Leaf Lamina ◽

Physic Nut ◽

Cotyledonary Nodes

Jatropha (Jatropha curcas L.) is an oil bearing crop growing in tropical and subtropical parts of the world. The present study was undertaken to investigate the effects of different plant growth regulators on in vitro callus induction in physic nut (J. curcus). In the present study, it was observed that all the explants viz., leaf lamina, petioles, nodal segments and cotyledonary nodes showed good callus induction responses on various culture media thus tried. Leaf lamina and petioles showed 100.0% callus induction responses on different MS media supplemented with auxins and cytokinins alone or in combinations whereas, nodal segments and cotyledonary nodes showed maximum 89.6% and 83.9% callus induction respectively. The presence of 2, 4-D in culture media with auxins or cytokinins was essential for good callus growth. Among different explants tried, leaf lamina was the best responding explants and MS-13 media supplemented with 5×10-6 M NAA and 10-5 M 2, 4-D is the best callusing and growth supporting medium. However, the regenerative competence of the callus tissues can differ depending on the type of explants used because certain types of plant tissues have more favorable regeneration responses than others. Callus induction rate from all explant types was highest than other reports. The results obtained in the present study would facilitate the high callus induction and regeneration responses in J. curcus for its improvement using biotechnological tools.

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